Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   WKI54_RS09175 Genome accession   NZ_CP149648
Coordinates   1741099..1741536 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain MJ04     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1736099..1746536
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WKI54_RS09150 (WKI54_09150) - 1737119..1738255 (+) 1137 WP_007609290.1 zinc-dependent alcohol dehydrogenase -
  WKI54_RS09155 (WKI54_09155) - 1738270..1738704 (+) 435 WP_014416871.1 RDD family protein -
  WKI54_RS09160 (WKI54_09160) - 1738776..1739099 (+) 324 WP_007409357.1 YckD family protein -
  WKI54_RS09165 (WKI54_09165) - 1739203..1740639 (+) 1437 WP_104843069.1 family 1 glycosylhydrolase -
  WKI54_RS09170 (WKI54_09170) nin/comJ 1740680..1741078 (-) 399 WP_017419379.1 competence protein ComJ Regulator
  WKI54_RS09175 (WKI54_09175) nucA/comI 1741099..1741536 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  WKI54_RS09180 (WKI54_09180) hxlB 1741867..1742424 (-) 558 WP_014416874.1 6-phospho-3-hexuloisomerase -
  WKI54_RS09185 (WKI54_09185) hxlA 1742421..1743056 (-) 636 WP_015239130.1 3-hexulose-6-phosphate synthase -
  WKI54_RS09190 (WKI54_09190) - 1743288..1743650 (+) 363 WP_104843070.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=960273 WKI54_RS09175 WP_003156588.1 1741099..1741536(-) (nucA/comI) [Bacillus velezensis strain MJ04]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=960273 WKI54_RS09175 WP_003156588.1 1741099..1741536(-) (nucA/comI) [Bacillus velezensis strain MJ04]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCCGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGTTACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAATAACGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATTAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641