Detailed information NTG0960263

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: WKI54_RS05590
• Genome accession: NZ_CP149648
• Coordinates: 1046867..1048012 (+)
• Length: 381 a.a.
• NCBI ID: WP_252052939.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain MJ04
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 1041867..1053012

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WKI54_RS05570 (WKI54_05570)	fnr	1042344..1043060 (-)	717	WP_252052937.1	fumarate/nitrate reduction transcriptional regulator Fnr	-
WKI54_RS05575 (WKI54_05575)	narK	1043156..1044346 (-)	1191	WP_017418712.1	nitrate transporter NarK	-
WKI54_RS05580 (WKI54_05580)	argS	1044480..1046150 (-)	1671	WP_017418711.1	arginine--tRNA ligase	-
WKI54_RS05585 (WKI54_05585)	-	1046147..1046575 (-)	429	WP_003151069.1	DUF1934 family protein	-
WKI54_RS05590 (WKI54_05590)	rapF	1046867..1048012 (+)	1146	WP_252052939.1	aspartate phosphatase	Regulator
WKI54_RS05595 (WKI54_05595)	-	1047996..1048115 (+)	120	WP_017418707.1	PhrC/PhrF family phosphatase-inhibitory pheromone	-
WKI54_RS05600 (WKI54_05600)	speB	1048238..1049110 (-)	873	WP_003151054.1	agmatinase	-
WKI54_RS05605 (WKI54_05605)	speE	1049170..1050000 (-)	831	WP_017418706.1	spermidine synthase	-
WKI54_RS05610 (WKI54_05610)	-	1050200..1052272 (+)	2073	WP_017418705.1	transglycosylase domain-containing protein	-
WKI54_RS05615 (WKI54_05615)	-	1052297..1052728 (-)	432	WP_017418704.1	hypothetical protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 45796.32 Da
• Isoelectric Point: 5.0749

>NTDB_id=960263 WKI54_RS05590 WP_252052939.1 1046867..1048012(+) (rapF) [Bacillus velezensis strain MJ04]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEEDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSELLADIDKKQARLTGRLDYYFNFFRGMYELECREYLSAIQFFKKAESKLTYVKDHIEKAEFYFKMSEVYYYMKQTYVS
MDYARQAYLIYKDEKKYNIRLTQCHSLFGANYLDVKQYDHAISHFQKAYTMAELEQQPQLMGRTLFNIALCHNNQNNKVE
AIPHLKEAIAVFEKAQMSHSLPQPYFLLTQIYFKLENIDNAYDYHKKGISICKEIDDIIYEEKFKFLEYLYMPDPRDTLI
NDCLHFLESKLMYADIEELALDVAKYYYTKEEYKKASVYFLKVEEARQQIEGGVNLYEIEV

Nucleotide

• Length: 1146 bp

>NTDB_id=960263 WKI54_RS05590 WP_252052939.1 1046867..1048012(+) (rapF) [Bacillus velezensis strain MJ04]
GTGGCGAGTGTTTTATCGTCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAGCTTGCAGGCCTGGAAGAAGATCAGGATCTTCATCTGTACTATT
CTCTCATGGAATTCCGCCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGAGCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGGCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGAATGCCGTGAATATCTTTCAGCGATTCAGTTTTTTAAGAAAGCTGAAAGCAAACTGACGTACG
TTAAAGATCATATAGAAAAAGCTGAATTTTACTTTAAAATGTCAGAAGTCTATTATTATATGAAACAAACTTATGTATCG
ATGGATTATGCGCGGCAGGCTTATTTAATTTATAAAGATGAAAAAAAATACAATATTAGATTAACCCAGTGCCATTCTTT
ATTTGGGGCTAATTATCTTGATGTAAAGCAATACGATCACGCGATTTCCCACTTTCAAAAGGCATATACGATGGCTGAAT
TGGAGCAGCAGCCGCAATTAATGGGGAGAACACTTTTTAACATCGCTTTATGCCATAATAATCAAAACAATAAAGTCGAG
GCTATTCCCCATTTAAAAGAAGCCATAGCTGTTTTTGAAAAAGCTCAAATGAGCCATTCACTTCCGCAGCCCTACTTCTT
ACTTACACAGATTTATTTTAAGTTAGAAAATATAGATAATGCGTACGATTATCATAAAAAAGGTATCTCAATATGTAAGG
AAATAGATGATATAATTTACGAGGAAAAATTTAAATTTTTGGAATATTTATATATGCCGGATCCAAGAGATACATTAATA
AACGATTGTCTGCATTTTCTTGAAAGTAAACTGATGTATGCTGATATTGAGGAATTAGCCCTTGATGTAGCTAAATATTA
TTACACAAAAGAAGAATATAAAAAAGCTTCTGTCTACTTTTTAAAAGTTGAAGAAGCCAGACAGCAGATCGAAGGAGGAG
TGAATTTATATGAAATTGAAGTATAA

Domains

Predicted by InterproScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (5-65)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	70.079	100	0.701
rapC	Bacillus subtilis subsp. subtilis str. 168	53.684	99.738	0.535