Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   WKI52_RS17495 Genome accession   NZ_CP149646
Coordinates   3520858..3521928 (-) Length   356 a.a.
NCBI ID   WP_014418378.1    Uniprot ID   A0AAP4DGV9
Organism   Bacillus velezensis strain MJ06     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3515858..3526928
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WKI52_RS17445 (WKI52_17445) - 3515917..3516501 (-) 585 WP_014418370.1 signal peptidase I -
  WKI52_RS17450 (WKI52_17450) tapA 3516473..3517144 (-) 672 WP_025649852.1 amyloid fiber anchoring/assembly protein TapA -
  WKI52_RS17455 (WKI52_17455) - 3517403..3517732 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  WKI52_RS17460 (WKI52_17460) - 3517773..3517952 (-) 180 WP_003153093.1 YqzE family protein -
  WKI52_RS17465 (WKI52_17465) comGG 3518009..3518386 (-) 378 WP_017418138.1 competence type IV pilus minor pilin ComGG Machinery gene
  WKI52_RS17470 (WKI52_17470) comGF 3518387..3518782 (-) 396 WP_025649850.1 competence type IV pilus minor pilin ComGF -
  WKI52_RS17475 (WKI52_17475) comGE 3518796..3519110 (-) 315 WP_017418140.1 competence type IV pilus minor pilin ComGE Machinery gene
  WKI52_RS17480 (WKI52_17480) comGD 3519094..3519531 (-) 438 WP_007612572.1 competence type IV pilus minor pilin ComGD Machinery gene
  WKI52_RS17485 (WKI52_17485) comGC 3519521..3519829 (-) 309 WP_014418376.1 competence type IV pilus major pilin ComGC Machinery gene
  WKI52_RS17490 (WKI52_17490) comGB 3519834..3520871 (-) 1038 WP_053284924.1 competence type IV pilus assembly protein ComGB Machinery gene
  WKI52_RS17495 (WKI52_17495) comGA 3520858..3521928 (-) 1071 WP_014418378.1 competence type IV pilus ATPase ComGA Machinery gene
  WKI52_RS17500 (WKI52_17500) - 3522121..3523071 (-) 951 WP_014418379.1 magnesium transporter CorA family protein -
  WKI52_RS17505 (WKI52_17505) - 3523217..3524518 (+) 1302 WP_017418142.1 hemolysin family protein -
  WKI52_RS17510 (WKI52_17510) - 3524556..3525386 (-) 831 WP_017418143.1 STAS domain-containing protein -
  WKI52_RS17520 (WKI52_17520) - 3525593..3525973 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  WKI52_RS17525 (WKI52_17525) - 3526210..3526455 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40529.81 Da        Isoelectric Point: 9.3485

>NTDB_id=960235 WKI52_RS17495 WP_014418378.1 3520858..3521928(-) (comGA) [Bacillus velezensis strain MJ06]
MKNIETYSRNIINEAYTARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKQQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=960235 WKI52_RS17495 WP_014418378.1 3520858..3521928(-) (comGA) [Bacillus velezensis strain MJ06]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAGGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGTG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGAAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACAGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCAGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCAGGCGCCGCATTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCGACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGCTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCCGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTTGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
TATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCTCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.303

100

0.823
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374