Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   WKI52_RS14465 Genome accession   NZ_CP149646
Coordinates   2877427..2877861 (+) Length   144 a.a.
NCBI ID   WP_007611576.1    Uniprot ID   A0A172XIP3
Organism   Bacillus velezensis strain MJ06     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2872427..2882861
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WKI52_RS14460 (WKI52_14460) - 2875927..2877138 (-) 1212 WP_025649439.1 cytochrome P450 -
  WKI52_RS14465 (WKI52_14465) nucA/comI 2877427..2877861 (+) 435 WP_007611576.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  WKI52_RS14470 (WKI52_14470) - 2877921..2878676 (+) 756 WP_110085565.1 YoaK family protein -
  WKI52_RS14475 (WKI52_14475) - 2878710..2879072 (-) 363 WP_007410383.1 hypothetical protein -
  WKI52_RS14480 (WKI52_14480) - 2879264..2880592 (-) 1329 WP_094295768.1 S8 family peptidase -
  WKI52_RS14485 (WKI52_14485) - 2880771..2881004 (+) 234 WP_021493648.1 hypothetical protein -
  WKI52_RS14490 (WKI52_14490) - 2881260..2881967 (+) 708 WP_151296526.1 poly-gamma-glutamate hydrolase family protein -
  WKI52_RS14495 (WKI52_14495) - 2882028..2882480 (+) 453 WP_110085567.1 OsmC family protein -
  WKI52_RS14500 (WKI52_14500) - 2882494..2882847 (-) 354 WP_007410379.1 multidrug efflux SMR transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15528.47 Da        Isoelectric Point: 7.2418

>NTDB_id=960224 WKI52_RS14465 WP_007611576.1 2877427..2877861(+) (nucA/comI) [Bacillus velezensis strain MJ06]
MNAFMKWAASLLLVISLQFGLTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADIRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=960224 WKI52_RS14465 WP_007611576.1 2877427..2877861(+) (nucA/comI) [Bacillus velezensis strain MJ06]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCCTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCGGAGAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATATCAGATATGTAACCCCGTCGGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGATACT
CCGACGGCACGAGAGTACTATTTATCGTTCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A172XIP3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

56.452

86.111

0.486