Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACI3MP_RS09535 Genome accession   NZ_CP173446
Coordinates   2020574..2022007 (-) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain 98232     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2015574..2027007
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI3MP_RS09500 (ACI3MP_09500) elbB 2016266..2016919 (+) 654 WP_001300411.1 isoprenoid biosynthesis glyoxalase ElbB -
  ACI3MP_RS09505 (ACI3MP_09505) mtgA 2016916..2017644 (+) 729 WP_000047073.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  ACI3MP_RS09510 (ACI3MP_09510) yrbL 2017641..2018273 (-) 633 WP_000620398.1 PhoP regulatory network protein YrbL -
  ACI3MP_RS09515 (ACI3MP_09515) npr 2018486..2018758 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  ACI3MP_RS09520 (ACI3MP_09520) rapZ 2018755..2019609 (-) 855 WP_000243741.1 RNase adapter RapZ -
  ACI3MP_RS09525 (ACI3MP_09525) ptsN 2019655..2020146 (-) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACI3MP_RS09530 (ACI3MP_09530) hpf 2020264..2020551 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  ACI3MP_RS09535 (ACI3MP_09535) rpoN/rpoN1 2020574..2022007 (-) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  ACI3MP_RS09540 (ACI3MP_09540) lptB 2022055..2022780 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  ACI3MP_RS09545 (ACI3MP_09545) lptA 2022787..2023344 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  ACI3MP_RS09550 (ACI3MP_09550) lptC 2023313..2023888 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  ACI3MP_RS09555 (ACI3MP_09555) kdsC 2023885..2024451 (-) 567 WP_000030006.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ACI3MP_RS09560 (ACI3MP_09560) kdsD 2024472..2025458 (-) 987 WP_001309787.1 arabinose-5-phosphate isomerase KdsD -
  ACI3MP_RS09565 (ACI3MP_09565) yrbG 2025472..2026449 (-) 978 WP_000922881.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=959219 ACI3MP_RS09535 WP_000809051.1 2020574..2022007(-) (rpoN/rpoN1) [Escherichia coli strain 98232]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=959219 ACI3MP_RS09535 WP_000809051.1 2020574..2022007(-) (rpoN/rpoN1) [Escherichia coli strain 98232]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACACTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGATACTGACCGCGCCATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGCCTGCTGATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGACTGATTATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACGGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGCGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCCTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465