Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   WDR05_RS13315 Genome accession   NZ_CP149444
Coordinates   2555277..2555687 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain HC-9     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2550277..2560687
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WDR05_RS13285 (WDR05_13275) - 2550389..2550529 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  WDR05_RS13290 (WDR05_13280) yqeF 2550835..2551566 (-) 732 WP_029726702.1 SGNH/GDSL hydrolase family protein -
  WDR05_RS13295 (WDR05_13285) cwlH 2551818..2552570 (-) 753 WP_029726701.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  WDR05_RS13300 (WDR05_13290) yqeD 2552757..2553383 (+) 627 WP_038427798.1 TVP38/TMEM64 family protein -
  WDR05_RS13305 (WDR05_13295) gnd 2553402..2554270 (-) 869 Protein_2572 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  WDR05_RS13310 (WDR05_13300) yqeB 2554522..2555244 (+) 723 WP_010886572.1 hypothetical protein -
  WDR05_RS13315 (WDR05_13305) nucA/comI 2555277..2555687 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  WDR05_RS13320 (WDR05_13310) - 2555883..2556233 (+) 351 Protein_2575 sigma-70 family RNA polymerase sigma factor -
  WDR05_RS13325 (WDR05_13315) spoIVCA 2556261..2556977 (-) 717 Protein_2576 site-specific DNA recombinase SpoIVCA -
  WDR05_RS13330 (WDR05_13320) yrkQ 2556976..2557936 (-) 961 Protein_2577 two-component system sensor histidine kinase YrkQ -
  WDR05_RS13335 (WDR05_13325) yrkP 2557923..2558618 (-) 696 WP_003229895.1 two-component response regulator YrkP -
  WDR05_RS13340 (WDR05_13330) yrkO 2558886..2560103 (+) 1218 WP_015251643.1 DUF418 domain-containing protein -
  WDR05_RS13345 (WDR05_13335) - 2560022..2560270 (+) 249 WP_119123070.1 hypothetical protein -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=959136 WDR05_RS13315 WP_009967785.1 2555277..2555687(-) (nucA/comI) [Bacillus subtilis strain HC-9]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=959136 WDR05_RS13315 WP_009967785.1 2555277..2555687(-) (nucA/comI) [Bacillus subtilis strain HC-9]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAGCAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGATAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACG
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCTGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529