Detailed information NTG0958650

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: ACI3L8_RS05930
• Genome accession: NZ_CP173391
• Coordinates: 1322198..1324399 (+)
• Length: 733 a.a.
• NCBI ID: WP_404971966.1
• Uniprot ID: -
• Organism: Vibrio campbellii strain LB3
• Function: DNA puliing through the inner membrane (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1317198..1329399

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACI3L8_RS05890 (ACI3L8_05890)	rplV	1317853..1318185 (-)	333	WP_005528535.1	50S ribosomal protein L22	-
ACI3L8_RS05895 (ACI3L8_05895)	rpsS	1318196..1318474 (-)	279	WP_005431614.1	30S ribosomal protein S19	-
ACI3L8_RS05900 (ACI3L8_05900)	rplB	1318496..1319320 (-)	825	WP_005528534.1	50S ribosomal protein L2	-
ACI3L8_RS05905 (ACI3L8_05905)	rplW	1319336..1319638 (-)	303	WP_004398471.1	50S ribosomal protein L23	-
ACI3L8_RS05910 (ACI3L8_05910)	rplD	1319635..1320237 (-)	603	WP_005435074.1	50S ribosomal protein L4	-
ACI3L8_RS05915 (ACI3L8_05915)	rplC	1320255..1320884 (-)	630	WP_005435072.1	50S ribosomal protein L3	-
ACI3L8_RS05920 (ACI3L8_05920)	rpsJ	1320899..1321210 (-)	312	WP_004410492.1	30S ribosomal protein S10	-
ACI3L8_RS05925 (ACI3L8_05925)	rpmE	1321684..1321902 (-)	219	WP_038890899.1	50S ribosomal protein L31	-
ACI3L8_RS05930 (ACI3L8_05930)	priA	1322198..1324399 (+)	2202	WP_404971966.1	primosomal protein N'	Machinery gene
ACI3L8_RS05935 (ACI3L8_05935)	cytR	1324603..1325613 (+)	1011	Protein_1142	DNA-binding transcriptional regulator CytR	-
ACI3L8_RS05940 (ACI3L8_05940)	-	1325782..1326333 (+)	552	WP_005429952.1	SPOR domain-containing protein	-
ACI3L8_RS05945 (ACI3L8_05945)	hslV	1326449..1327000 (+)	552	WP_005435051.1	ATP-dependent protease subunit HslV	-
ACI3L8_RS05950 (ACI3L8_05950)	hslU	1327030..1328361 (+)	1332	WP_010646389.1	HslU--HslV peptidase ATPase subunit	-

Sequence

Protein

• Length: 733 a.a.
• Molecular weight: 82000.54 Da
• Isoelectric Point: 8.7438

>NTDB_id=958650 ACI3L8_RS05930 WP_404971966.1 1322198..1324399(+) (priA) [Vibrio campbellii strain LB3]
MRPSIARVALPVPLDKQFDYLIPSHLFPVIGGRVSVPFGRQTLVGIVTAMVNHSDFPKEQLKAIKAVLDAQPVWPEKLYS
LLTWCSQFYQYPLGDTLHNAMPAALRKGKPADFATLQEWQITASGKDKLMQGLGRAVKQQRALQMLVNGSVPHQEFIDQE
IGSTVLKSLEEKGWVERIEKKPTITKWGQQVEFDVEKPKLNHEQALAIASVNSQTGFACYLLEGVTGSGKTEVYLNLIKP
VLEQGKQALVLVPEIGLTPQTINRFKRRFNVPVDVIHSGLNDTERLNAWLSARDKAAGIIIGTRSALLTPFADLGIIIVD
EEHDSSYKQQDSLRYHARDVAVMRAHKEQVPIVLGSATPALETLHNALTGKYHHLTLTQRAGSAVPTTNKVLDVKGLYLD
SGLSAPLIAEMRKHLKAGNQVMLFLNRRGFSPALMCHECGWIAECKRCDAYYTFHQYSNEIRCHHCGSQQPVINQCQGCG
STQLVTVGVGTEQLELQLAELFPEYKAIRIDRDSTRRKGSLEDALESIRKGEYQILIGTQMLAKGHHFPNVTLVALLDVD
GSLYSSDFRASERLAQLFIQVAGRAGRASKPGEVILQTHHPEHSLLQALLQKDYRHFAMTALEERKLAQLPPYSFLTLFK
AEANQSEVVEDFLRQVRFTLESHPLFDDNCMVLGPTPSPLAKRAGKYRWQLLLQTQHRSLMQKLLASAKPAIELLPNAKK
VRWNLDIEPQDLS

Nucleotide

• Length: 2202 bp

>NTDB_id=958650 ACI3L8_RS05930 WP_404971966.1 1322198..1324399(+) (priA) [Vibrio campbellii strain LB3]
ATGCGTCCATCCATCGCCCGAGTAGCACTGCCAGTCCCGCTCGACAAACAGTTTGACTATCTCATACCTAGTCACCTTTT
CCCTGTGATTGGCGGACGCGTTTCTGTGCCTTTTGGTCGGCAGACTTTGGTTGGCATCGTGACAGCAATGGTTAATCATT
CAGACTTTCCTAAAGAGCAACTGAAAGCGATTAAAGCGGTATTAGATGCCCAACCAGTTTGGCCAGAAAAACTCTATTCT
TTACTGACTTGGTGTAGCCAGTTTTACCAATATCCACTTGGTGACACACTTCATAATGCAATGCCAGCTGCCCTGCGTAA
GGGAAAACCTGCCGATTTCGCGACATTACAAGAGTGGCAAATCACTGCGTCGGGTAAAGATAAGCTGATGCAAGGCTTAG
GGCGTGCAGTCAAGCAACAGCGTGCTTTGCAGATGCTGGTCAATGGTTCAGTGCCACACCAAGAGTTCATCGACCAAGAA
ATTGGCTCTACCGTACTTAAGTCTTTGGAAGAGAAAGGCTGGGTCGAACGCATAGAGAAAAAGCCGACGATCACTAAGTG
GGGCCAACAAGTTGAGTTCGATGTTGAAAAACCAAAGCTCAACCACGAACAAGCTCTGGCGATTGCTAGCGTAAACAGCC
AAACTGGCTTTGCCTGCTATTTATTAGAGGGCGTTACAGGTTCCGGCAAAACCGAAGTTTACCTTAACCTCATTAAGCCA
GTACTTGAACAAGGTAAGCAAGCTTTGGTGCTCGTCCCTGAGATAGGCCTGACTCCCCAAACCATTAACCGCTTCAAGCG
TCGCTTCAATGTGCCTGTGGATGTCATTCACTCTGGGTTAAATGATACCGAGCGACTCAACGCCTGGCTTTCTGCGCGAG
ACAAAGCCGCTGGCATCATTATTGGTACGCGCTCTGCCCTACTGACGCCTTTTGCTGATCTTGGCATTATCATTGTCGAT
GAAGAACACGACTCGTCTTATAAACAACAAGATAGCCTACGCTACCATGCTCGTGATGTTGCGGTGATGCGTGCACATAA
AGAGCAAGTGCCTATTGTCCTCGGCTCTGCCACGCCAGCCTTAGAAACACTGCACAATGCACTAACGGGCAAGTACCACC
ATTTAACTCTGACACAACGCGCCGGTTCTGCCGTTCCGACCACTAACAAGGTACTAGATGTTAAAGGCCTCTATCTAGAC
AGTGGCTTATCCGCGCCATTGATCGCTGAAATGCGCAAACATCTAAAAGCGGGCAACCAAGTGATGCTGTTCTTAAACCG
TCGCGGCTTCTCACCGGCGTTAATGTGCCATGAATGTGGCTGGATTGCGGAATGTAAACGCTGTGATGCTTACTACACCT
TCCACCAGTACAGCAATGAGATTCGCTGTCACCATTGTGGCTCACAGCAGCCTGTGATTAATCAGTGCCAAGGTTGTGGT
TCGACTCAGTTGGTCACGGTGGGTGTGGGTACCGAACAGCTTGAGTTGCAACTGGCTGAGCTCTTCCCAGAATACAAAGC
CATTCGCATTGATAGAGACAGCACGCGCCGTAAAGGCAGTTTAGAAGATGCGCTCGAATCGATTCGTAAAGGCGAATACC
AAATTCTGATTGGCACGCAGATGTTAGCAAAAGGCCACCACTTTCCAAATGTTACGCTTGTTGCGCTACTCGATGTTGAT
GGCTCCCTCTACAGTAGTGACTTCCGCGCCTCCGAGAGACTGGCTCAACTGTTTATTCAGGTCGCAGGTCGCGCAGGTCG
CGCAAGCAAACCCGGTGAAGTGATACTGCAAACTCACCACCCAGAACACAGTTTACTGCAGGCTCTACTACAGAAAGACT
ATCGCCACTTTGCTATGACAGCACTGGAGGAGCGTAAACTCGCGCAACTGCCACCTTATAGCTTCTTGACCTTATTTAAA
GCAGAAGCCAACCAAAGCGAAGTGGTCGAAGACTTTTTACGTCAGGTGCGTTTTACCCTTGAATCGCACCCATTGTTCGA
TGATAACTGTATGGTGTTGGGGCCAACACCATCACCACTGGCTAAGCGCGCTGGCAAATATCGATGGCAGCTTTTACTGC
AAACTCAGCATCGCTCATTGATGCAAAAGTTATTAGCCAGTGCAAAACCTGCTATTGAGTTGTTGCCAAATGCCAAGAAA
GTTCGCTGGAATTTAGACATAGAGCCACAGGATCTCAGCTAG

Domains

Predicted by InterProScan.

• 3DNA-binding domain (3BD) (PriA_3primeBD) (7-102)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (445-471)

• Helicase conserved C-terminal domain (Helicase_C) (499-588)

• Primosomal protein N C-terminal domain (PriA_C) (632-729)

• DEAD/DEAH box helicase (DEAD) (219-364)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Acinetobacter baumannii D1279779	44.762	100	0.449