Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   ACIX3D_RS05630 Genome accession   NZ_CP173318
Coordinates   1236053..1238251 (-) Length   732 a.a.
NCBI ID   WP_005597822.1    Uniprot ID   A0ABM6X3R9
Organism   Actinobacillus pleuropneumoniae strain APL2210214E     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1231053..1243251
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIX3D_RS05610 (ACIX3D_05615) - 1232140..1232910 (-) 771 WP_005597814.1 glycosyltransferase family 25 protein -
  ACIX3D_RS05615 (ACIX3D_05620) - 1232907..1234115 (-) 1209 WP_005597816.1 lipopolysaccharide biosynthesis protein -
  ACIX3D_RS05620 (ACIX3D_05625) - 1234093..1235058 (-) 966 WP_005597818.1 polysaccharide pyruvyl transferase family protein -
  ACIX3D_RS05625 (ACIX3D_05630) - 1235214..1235954 (+) 741 WP_005597819.1 YdcF family protein -
  ACIX3D_RS05630 (ACIX3D_05635) priA 1236053..1238251 (-) 2199 WP_005597822.1 primosomal protein N' Machinery gene
  ACIX3D_RS05635 (ACIX3D_05640) - 1238354..1239511 (-) 1158 WP_005597823.1 chorismate mutase -
  ACIX3D_RS05640 (ACIX3D_05645) prlC 1239634..1241667 (-) 2034 WP_005597825.1 oligopeptidase A -
  ACIX3D_RS05645 (ACIX3D_05650) pflA 1241810..1242547 (-) 738 WP_005597829.1 pyruvate formate lyase 1-activating protein -

Sequence

Protein


Download         Length: 732 a.a.        Molecular weight: 82549.18 Da        Isoelectric Point: 8.7569

>NTDB_id=958053 ACIX3D_RS05630 WP_005597822.1 1236053..1238251(-) (priA) [Actinobacillus pleuropneumoniae strain APL2210214E]
MALPVPLHRYFDYLLPSFLSAVKGVRVEVPFGNQTKIGIVVDFPETSDVPEDKLKPIKAVLDLEPIFDKEIWTLLRWAAR
YYHAPIGEVAINALPVKLRNGDSTERSQPDYFAVTEAGRAALLSGENKRAKKQQDLLAKLAEIANFFEKPTACSQSAWKG
LLEKNYIEPVNVPFELQHWQKKLQNRPLVNLHNRLVLNKQQTLVVSRLAVQNGFEAFLLNGVTGSGKTEVYLQVIEEVLK
RGEQVLVLVPEIGLTPQTVQRFKARFNVEIDVLHSNMNETERLNAWLRAKNGESAIVIGTRSALFSQFEKLGLIIIDEEH
DSSFKQQDGWRYHARDLAILRAKNLHIPIILGSATPSLESVQNVQNGKFIELSLTARAGNAQQAKQQLIDLKTQRITAGL
SDRLLAMMKQHLEQGNQVMLFLNRRGFAPVLLCHECGWMCECDACEKPYTYHQKQKILRCHHCASQRVIPRQCGHCGSTN
LITTGVGTEQLEQVLSEQFPTYQISRIDRDSTARKGALENHLNDIREGKSQILIGTQMLAKGHHFPNVTLVAIVNVDSAL
FSTDFRAEERLAQLYVQVAGRAGRAEKQGEVVLQTHYPEHPLLKTLLEQGYLAFAQEALKMRKVMGLPPFAAQVLLRATG
KDNQQVVNLLQNLTAYFQQKCTELGLQDIQLLPPFSAPMAKKAGHYRWLLLIQHRSRGVLQQLIDQFDLDKANLGLQNNI
RLTLDIDPQEIG

Nucleotide


Download         Length: 2199 bp        

>NTDB_id=958053 ACIX3D_RS05630 WP_005597822.1 1236053..1238251(-) (priA) [Actinobacillus pleuropneumoniae strain APL2210214E]
GTGGCATTGCCCGTGCCGTTACATCGTTATTTTGATTATTTACTGCCGTCGTTTTTGTCGGCAGTCAAAGGTGTGCGTGT
TGAAGTGCCTTTCGGTAATCAAACGAAAATCGGGATTGTAGTGGATTTTCCCGAAACGAGCGATGTGCCTGAAGATAAGC
TCAAACCGATTAAAGCGGTATTAGATTTAGAACCGATCTTTGACAAGGAAATTTGGACACTGCTTCGTTGGGCGGCTCGT
TATTATCATGCGCCTATCGGCGAAGTGGCGATCAATGCCTTGCCGGTGAAATTGCGTAACGGCGATAGTACCGAAAGATC
GCAACCGGATTACTTTGCGGTTACTGAGGCAGGGCGAGCAGCATTGCTCTCGGGTGAGAACAAGCGGGCCAAAAAACAGC
AAGATTTACTGGCGAAACTGGCAGAAATTGCAAATTTTTTTGAAAAACCAACCGCTTGTAGTCAATCGGCTTGGAAAGGG
CTATTGGAAAAAAACTATATCGAACCGGTTAATGTGCCGTTTGAGCTGCAACATTGGCAAAAAAAATTGCAAAATCGACC
GCTTGTAAACTTGCATAATCGACTGGTATTAAATAAACAGCAAACCCTAGTGGTGAGCCGCTTAGCAGTACAAAACGGTT
TCGAAGCTTTTTTATTAAATGGCGTAACCGGTTCAGGCAAAACCGAAGTGTATTTGCAAGTGATCGAAGAGGTGTTAAAG
CGAGGCGAGCAAGTATTGGTGTTAGTGCCGGAAATCGGGCTGACTCCGCAAACGGTGCAACGTTTTAAAGCCCGTTTTAA
TGTTGAAATTGATGTTCTTCATTCGAATATGAATGAAACAGAGCGATTAAATGCTTGGTTGCGAGCAAAAAACGGCGAAA
GTGCGATTGTGATCGGCACGCGTTCGGCATTGTTCAGCCAGTTTGAGAAATTGGGCTTAATCATTATTGATGAAGAACAC
GATAGTTCTTTCAAACAACAAGACGGCTGGCGTTATCACGCAAGAGATTTGGCAATTTTACGGGCAAAAAATTTACATAT
CCCGATTATTTTGGGTTCTGCAACGCCAAGTTTGGAAAGCGTACAAAACGTTCAAAACGGTAAGTTTATTGAGCTATCTT
TAACCGCTCGTGCCGGTAATGCTCAACAGGCGAAACAGCAATTGATTGATTTAAAAACACAGCGTATTACCGCCGGACTT
TCCGACCGCTTATTAGCGATGATGAAGCAGCATTTAGAGCAAGGCAATCAGGTAATGTTGTTTTTAAATCGCCGCGGCTT
TGCACCGGTTTTACTTTGTCACGAGTGTGGCTGGATGTGTGAATGTGACGCTTGTGAAAAGCCTTACACTTATCATCAGA
AACAGAAAATCTTGCGTTGTCATCATTGTGCTTCACAGCGAGTGATCCCTCGCCAGTGCGGGCATTGCGGCTCAACCAAT
CTGATTACCACCGGAGTGGGGACGGAGCAGTTGGAGCAAGTGTTAAGTGAGCAATTCCCAACTTACCAAATTAGCCGTAT
CGACCGAGATTCCACCGCTCGTAAAGGGGCATTGGAAAATCACTTAAACGATATCCGAGAAGGCAAAAGCCAAATTTTGA
TTGGCACGCAAATGCTAGCGAAAGGACACCATTTTCCGAATGTAACTTTAGTGGCGATTGTGAATGTAGATTCGGCACTG
TTTTCGACCGACTTCCGTGCGGAAGAACGTTTGGCACAGCTTTATGTGCAAGTGGCGGGACGTGCCGGACGGGCGGAAAA
GCAGGGCGAAGTGGTATTACAAACTCATTATCCGGAACATCCGTTGCTCAAGACTTTGTTAGAACAAGGTTATTTGGCGT
TTGCACAAGAAGCGTTAAAAATGCGTAAAGTGATGGGCTTGCCGCCGTTTGCTGCGCAAGTGTTGTTACGGGCAACCGGC
AAAGATAATCAGCAAGTGGTAAATCTTTTGCAAAATTTGACCGCTTATTTTCAGCAAAAATGTACGGAACTCGGTTTGCA
GGATATCCAGCTTCTACCGCCTTTTTCCGCACCGATGGCTAAAAAAGCGGGGCATTACCGCTGGCTTTTATTAATTCAAC
ATCGCTCAAGAGGCGTATTACAACAGTTGATCGATCAATTTGATTTGGATAAAGCCAATTTAGGCTTACAAAATAATATT
CGTTTAACCTTAGATATTGATCCGCAAGAAATCGGCTAA

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(215-363)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(442-467)

Primosomal protein N C-terminal domain (PriA_C)

(629-728)

Helicase conserved C-terminal domain (Helicase_C)

(491-585)

3DNA-binding domain (3BD) (PriA_3primeBD)

(2-95)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

42.722

100

0.433