Detailed information NTG0957677

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: ACIO08_RS08970
• Genome accession: NZ_CP173233
• Coordinates: 1838277..1840463 (+)
• Length: 728 a.a.
• NCBI ID: WP_410678033.1
• Uniprot ID: -
• Organism: Avibacterium paragallinarum strain npAP/OH-USA/20230213/S1-2
• Function: DNA puliing through the inner membrane (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1833277..1845463

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACIO08_RS08940 (ACIO08_08940)	tmk	1833457..1834086 (+)	630	WP_410678027.1	dTMP kinase	-
ACIO08_RS08945 (ACIO08_08945)	-	1834092..1835096 (+)	1005	WP_410679353.1	DNA polymerase III subunit delta'	-
ACIO08_RS08950 (ACIO08_08950)	-	1835106..1835888 (+)	783	WP_410678029.1	TatD family hydrolase	-
ACIO08_RS08955 (ACIO08_08955)	-	1835917..1836444 (+)	528	WP_410678031.1	DUF1523 family protein	-
ACIO08_RS08960 (ACIO08_08960)	-	1836510..1837796 (+)	1287	WP_410678032.1	hemolysin family protein	-
ACIO08_RS08965 (ACIO08_08965)	rpmE	1837863..1838075 (-)	213	WP_103854262.1	50S ribosomal protein L31	-
ACIO08_RS08970 (ACIO08_08970)	priA	1838277..1840463 (+)	2187	WP_410678033.1	primosomal protein N'	Machinery gene
ACIO08_RS08975 (ACIO08_08975)	-	1840654..1841313 (+)	660	WP_410678034.1	SPOR domain-containing protein	-
ACIO08_RS08980 (ACIO08_08980)	msrP	1841539..1842498 (+)	960	WP_410678035.1	protein-methionine-sulfoxide reductase catalytic subunit MsrP	-
ACIO08_RS08985 (ACIO08_08985)	-	1842498..1843073 (+)	576	WP_410678036.1	sulfite oxidase heme-binding subunit YedZ	-
ACIO08_RS08990 (ACIO08_08990)	rlmC	1843070..1844242 (-)	1173	WP_410678037.1	23S rRNA (uracil(747)-C(5))-methyltransferase RlmC	-
ACIO08_RS08995 (ACIO08_08995)	nagZ	1844242..1845288 (-)	1047	WP_410678038.1	beta-N-acetylhexosaminidase	-

Sequence

Protein

• Length: 728 a.a.
• Molecular weight: 82744.59 Da
• Isoelectric Point: 8.5334

>NTDB_id=957677 ACIO08_RS08970 WP_410678033.1 1838277..1840463(+) (priA) [Avibacterium paragallinarum strain npAP/OH-USA/20230213/S1-2]
MNLVRVALAVPLFQFFDYLLPEEMQPVRGGRVLVPFGRQQRVGIVVDFPAQSEVASEQLKPICQVLDEENLFNEPLWQLI
YWAANYYQAPLGEALFQALPVKLRQGESAVENDRTFLRITPQGEQALRENLLKRAKKQQEALHSLAQSPQEKGQTPFASN
IWHSLKEKGYVEEYEQSNPIRPWQALEQPIVNEENKHRLNKEQALALGRILFHQGFAVWLIDGVTGSGKTEIYLQTIEET
LKKGQQVLVLVPEIGLTPQTVQRFKARFNVEIDVLHSNLNDTQRLQAWQRAKSGQSAVVIGTRSALFTQFSNLGLIVIDE
EHDGSFKQQEGWRYHARDLAVVYAKLLNIPILLGSATPSLESLNNVKLGKYQHLILQKRAGNSTALQQFVIDLKHQRIQN
GLSDALANKMQEHLQQGNQVLLFLNRRGFAPVLFCHECGWIATCQHCEKPYTYHQHQRVLRCHHCSSQKPIPMQCGHCGS
THLMTTGLGTEQLEETLKQRFPDYVVTRIDRDSTARKGTLEKHLQDIQQGKSQILIGTQMLAKGHHFPNVALVGLLNVDN
ALFSLDFRAEERLAQLYVQVAGRAGRSEKHGQVVLQTHYPDHPLLRSLLKEGYGEFAQQALHLREQMGLPPYSSQALFRA
QSRHSEQAEQFLQQIAATLQHLQIDGLQIYGPMPAPFSKKAGAYRWQLLVQHSSRAVLHQALSQFQANMPQKSHQVRWIL
DVDPLDLT

Nucleotide

• Length: 2187 bp

>NTDB_id=957677 ACIO08_RS08970 WP_410678033.1 1838277..1840463(+) (priA) [Avibacterium paragallinarum strain npAP/OH-USA/20230213/S1-2]
ATGAATCTTGTCCGTGTCGCGTTGGCAGTGCCGTTGTTTCAATTTTTTGATTATTTACTCCCCGAAGAGATGCAACCCGT
GCGTGGTGGGCGGGTGCTTGTGCCTTTTGGACGACAACAACGGGTTGGCATTGTGGTGGATTTCCCCGCACAAAGTGAGG
TCGCCTCCGAACAGCTCAAACCCATTTGCCAAGTGCTAGATGAAGAAAATCTCTTTAATGAACCCCTGTGGCAGTTGATC
TATTGGGCTGCGAATTATTATCAAGCCCCACTGGGTGAGGCGTTATTTCAAGCCTTGCCTGTGAAATTGCGACAGGGAGA
AAGTGCGGTGGAAAATGACCGCACTTTTTTACGCATTACCCCGCAGGGGGAGCAGGCATTGCGGGAGAATTTGCTAAAGC
GGGCGAAAAAACAACAAGAAGCCTTGCATTCACTCGCCCAATCCCCGCAGGAAAAAGGGCAAACGCCGTTTGCTTCTAAC
ATTTGGCATAGCCTCAAGGAAAAGGGCTATGTGGAGGAATACGAACAATCCAACCCTATTCGTCCTTGGCAAGCCCTTGA
ACAGCCGATCGTTAATGAAGAAAACAAACATCGTTTAAACAAAGAGCAAGCCCTCGCACTGGGTCGAATTTTGTTTCATC
AAGGTTTTGCCGTGTGGTTGATTGATGGGGTTACGGGATCGGGCAAAACGGAAATCTATCTGCAAACCATTGAAGAAACC
TTGAAAAAGGGGCAGCAAGTGCTGGTGCTTGTGCCTGAGATTGGCTTAACCCCGCAAACTGTACAGCGCTTTAAGGCGCG
TTTTAATGTGGAAATTGATGTTCTACATTCTAATTTGAACGATACGCAACGCTTGCAGGCTTGGCAACGAGCGAAATCGG
GGCAAAGTGCGGTGGTAATCGGCACAAGATCGGCGTTGTTCACTCAATTTTCCAATTTGGGCTTGATCGTGATCGATGAA
GAACACGACGGCTCATTCAAACAACAAGAGGGTTGGCGTTATCACGCGAGGGATCTGGCAGTGGTGTATGCCAAATTGCT
GAATATTCCGATTTTATTGGGATCGGCAACGCCAAGTTTGGAAAGCCTGAATAATGTGAAGCTGGGCAAATATCAGCATC
TTATTTTGCAAAAACGGGCAGGAAATAGCACCGCACTTCAACAATTTGTCATTGATTTGAAACATCAGCGGATTCAAAAT
GGGCTGTCTGATGCCTTGGCAAATAAAATGCAAGAACATTTGCAACAGGGCAATCAAGTGCTGTTATTTCTTAATCGGCG
TGGCTTTGCCCCCGTTTTGTTTTGCCACGAATGTGGTTGGATCGCGACCTGCCAGCATTGTGAAAAGCCCTATACTTATC
ATCAACATCAGCGGGTGTTGCGTTGCCATCATTGCAGTTCACAAAAGCCGATCCCAATGCAATGCGGGCATTGTGGTTCA
ACCCATTTGATGACCACAGGGCTTGGTACAGAACAGCTGGAAGAAACCCTAAAACAACGCTTCCCTGATTATGTGGTAAC
CCGAATTGATCGCGACAGCACCGCTCGTAAAGGCACATTGGAGAAGCATCTGCAAGATATTCAGCAAGGAAAGAGCCAAA
TTCTCATCGGCACGCAAATGTTGGCGAAAGGACATCATTTCCCCAATGTTGCCCTCGTTGGGCTATTAAATGTAGATAAT
GCCTTGTTTTCATTAGATTTTCGTGCGGAAGAACGACTTGCTCAGCTTTATGTGCAAGTGGCAGGGCGTGCGGGGCGAAG
CGAAAAACACGGGCAAGTGGTGCTACAAACCCATTATCCCGATCACCCTTTATTACGCTCATTATTGAAAGAGGGCTATG
GCGAGTTTGCTCAGCAAGCCTTGCATTTACGCGAACAAATGGGACTGCCGCCGTACAGCTCGCAGGCGTTATTTCGCGCT
CAAAGCCGTCATTCGGAGCAAGCAGAGCAGTTTTTGCAACAGATCGCCGCGACATTACAGCATTTACAGATCGATGGCTT
GCAAATTTACGGCCCAATGCCTGCCCCATTCAGCAAAAAGGCAGGGGCGTATCGCTGGCAATTATTGGTGCAACACTCAT
CAAGAGCGGTGTTACATCAGGCATTAAGCCAATTCCAAGCCAATATGCCGCAAAAATCCCACCAAGTTCGCTGGATTTTA
GATGTCGATCCCCTTGATTTAACCTAA

Domains

Predicted by InterProScan.

• Primosomal protein N C-terminal domain (PriA_C) (631-724)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (5-100)

• DEAD/DEAH box helicase (DEAD) (203-364)

• Helicase conserved C-terminal domain (Helicase_C) (501-587)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (444-470)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Acinetobacter baumannii D1279779	45.257	100	0.459
priA	Bacillus subtilis subsp. subtilis str. 168	32.962	100	0.365