Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   ACIQTD_RS02880 Genome accession   NZ_CP173185
Coordinates   558865..561093 (+) Length   742 a.a.
NCBI ID   WP_058208573.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. lactis strain MGEL24009     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 553865..566093
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIQTD_RS02845 - 554354..555022 (+) 669 WP_003129542.1 hypothetical protein -
  ACIQTD_RS02850 - 555193..556089 (+) 897 WP_003129543.1 SPFH domain-containing protein -
  ACIQTD_RS02860 - 556464..556724 (+) 261 WP_003129544.1 SemiSWEET family transporter -
  ACIQTD_RS02870 rnz 557082..558005 (+) 924 WP_003129545.1 ribonuclease Z -
  ACIQTD_RS02875 - 557998..558699 (+) 702 WP_270250564.1 SDR family oxidoreductase -
  ACIQTD_RS02880 recJ 558865..561093 (+) 2229 WP_058208573.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  ACIQTD_RS02885 - 561216..561728 (+) 513 WP_012897343.1 adenine phosphoribosyltransferase -
  ACIQTD_RS02890 rpoE 561945..562508 (+) 564 WP_012897344.1 DNA-directed RNA polymerase subunit delta -
  ACIQTD_RS02895 mltG 562645..564297 (+) 1653 WP_270250566.1 endolytic transglycosylase MltG -
  ACIQTD_RS02900 greA 564357..564830 (+) 474 WP_033899645.1 transcription elongation factor GreA -
  ACIQTD_RS02905 - 565007..565462 (+) 456 WP_010905471.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 742 a.a.        Molecular weight: 83556.66 Da        Isoelectric Point: 4.7534

>NTDB_id=957005 ACIQTD_RS02880 WP_058208573.1 558865..561093(+) (recJ) [Lactococcus lactis subsp. lactis strain MGEL24009]
MIKAKYDWKVADTAISEDFLKIAKKHKLDELTSRVLYQRGIHAEAEIEQFLKPSLENLHDPFLLHDMEKATGRILSAIEQ
GENILIYGDYDADGMTASSVMKSALDELGAEAQVYLPNRFTDGYGPNLDVYQYYIKNENINLIITVDNGVAGLEAITWAQ
ENGVDVIVTDHHSIPDQLPPAYAIVHPEHPDSQYPFKYLAGVGVAFKVACALLEYAPSEMLDLVAIGTIADMVSLTDENR
ILVAHGLKVLAQTERAGLQELMKYAGVDFDKITEETVGFQIAPRLNALGRLDDPNPAIELLTGWDEDEAHEIAKMIDQKN
SERKEIVEKIHNEALSMLTDEPVQILYHKDWHKGVLGIVAGRLLEAIHKPVIMLAQEDGILRGSARSIENFDIFKALNAH
RELFIAFGGHKQAAGMTLSLENVEAVKKAMIDYIVDNHLDMSKKSPLEIADRCHLDDISLSTITNLAKLAPFGMDNPKPR
FLIEDYKVIQSRSMGKDNAHLKLKIQEEKQQIDAVYFQHGSEELEFEQAQTKLVATLSSNSWNGNTSLQLMIEDADSVGV
ELLDIRSKQIPIPKEANIFSQNQLKHGIMEDVLVIEETPGDLTGLSVLKEAVSKAASDDFKMIYFKNKITDSYYLTGSGT
REEFARLYKAIYQFPEFDIRYKLKSLADYLKIPHLLLVKMIKIFEELEFVSIDNGLMTVNKTADKREISESTIYQELEKI
VKMQELFALAPVKEIYQNLLEK

Nucleotide


Download         Length: 2229 bp        

>NTDB_id=957005 ACIQTD_RS02880 WP_058208573.1 558865..561093(+) (recJ) [Lactococcus lactis subsp. lactis strain MGEL24009]
ATGATAAAAGCAAAATATGATTGGAAAGTGGCTGATACAGCCATTTCCGAAGATTTTCTAAAGATAGCGAAAAAACATAA
ATTAGATGAATTGACAAGTCGCGTGCTTTATCAAAGAGGAATTCATGCAGAAGCTGAAATTGAGCAATTTTTAAAGCCAA
GTTTAGAAAATTTACATGATCCTTTTCTACTTCATGATATGGAAAAAGCAACAGGGCGTATTTTATCAGCAATTGAGCAA
GGTGAAAACATCTTAATTTATGGTGACTATGATGCTGACGGAATGACTGCATCATCAGTCATGAAATCCGCTCTGGATGA
ATTAGGAGCTGAGGCTCAAGTTTATTTACCCAATCGTTTTACTGACGGTTACGGGCCAAATCTTGACGTTTATCAGTACT
ATATTAAGAATGAAAATATCAATCTTATTATTACTGTAGATAATGGGGTTGCTGGCTTGGAGGCAATTACCTGGGCACAG
GAAAATGGAGTGGATGTAATTGTTACCGACCATCACTCAATTCCTGACCAACTTCCCCCTGCTTATGCCATTGTCCACCC
CGAACATCCTGACAGTCAATATCCTTTCAAATATTTGGCAGGAGTTGGTGTCGCCTTCAAAGTTGCCTGTGCTCTTTTAG
AATATGCACCCAGTGAAATGCTTGATTTAGTTGCAATTGGTACAATTGCTGACATGGTCAGTTTGACTGATGAAAACCGA
ATTTTAGTGGCTCATGGTCTAAAAGTTTTGGCCCAAACAGAGCGTGCCGGCCTACAAGAATTAATGAAGTATGCTGGGGT
TGATTTTGATAAAATCACTGAGGAAACGGTTGGTTTTCAAATTGCTCCTCGTTTAAACGCTTTGGGACGTCTCGATGACC
CAAACCCAGCCATTGAATTGCTGACAGGTTGGGATGAAGATGAAGCGCATGAAATTGCCAAAATGATTGACCAAAAAAAT
TCAGAGCGAAAAGAAATTGTGGAAAAAATTCACAATGAAGCTCTGAGTATGCTGACAGATGAACCTGTTCAGATTTTATA
TCATAAGGACTGGCATAAAGGTGTCTTAGGAATTGTTGCGGGTCGCTTGTTAGAAGCCATACATAAGCCAGTAATCATGC
TGGCCCAAGAAGATGGGATTTTGCGTGGGTCTGCTCGGTCAATCGAAAATTTTGATATTTTCAAGGCATTAAATGCCCAT
CGTGAACTTTTTATTGCTTTTGGTGGTCATAAACAAGCCGCAGGAATGACTTTAAGTCTGGAAAACGTAGAAGCAGTTAA
AAAGGCGATGATTGATTATATCGTGGACAATCATTTAGATATGTCTAAAAAAAGTCCCTTAGAAATTGCTGACCGTTGCC
ACTTAGACGATATTTCGCTCTCAACAATCACTAATTTAGCAAAATTGGCTCCCTTTGGCATGGATAATCCTAAGCCTCGA
TTTTTAATCGAAGATTATAAGGTGATTCAAAGCCGAAGTATGGGGAAAGACAATGCTCATCTTAAATTAAAAATTCAGGA
AGAAAAGCAACAAATTGATGCCGTTTATTTCCAACATGGTTCAGAAGAATTGGAATTCGAACAGGCACAAACAAAATTAG
TCGCAACACTTTCAAGTAATAGTTGGAATGGAAATACGAGTCTTCAATTAATGATTGAAGATGCTGATTCAGTTGGTGTC
GAACTTCTTGATATAAGAAGTAAACAAATTCCTATCCCAAAAGAGGCCAATATTTTTTCTCAAAATCAGCTAAAACATGG
TATAATGGAAGATGTGCTTGTCATCGAAGAGACACCTGGAGATTTAACAGGTTTGTCAGTATTAAAAGAAGCTGTCAGTA
AAGCAGCGTCGGATGATTTTAAAATGATTTATTTTAAAAATAAAATCACTGATAGCTATTATTTAACAGGAAGTGGAACA
CGAGAAGAATTTGCAAGACTTTATAAAGCAATTTATCAATTTCCAGAATTTGATATTCGCTATAAATTAAAATCACTTGC
GGATTATTTGAAAATCCCTCATCTCTTGCTTGTCAAGATGATAAAAATCTTTGAGGAACTTGAATTTGTCAGCATTGACA
ATGGCTTAATGACCGTTAATAAGACGGCCGATAAACGCGAAATTTCTGAGAGTACAATTTATCAAGAACTAGAAAAAATT
GTAAAAATGCAAGAACTTTTTGCTTTAGCACCCGTCAAAGAAATTTATCAAAATTTATTAGAAAAATAA

Domains

Predicted by InterProScan.

RecJ OB domain (RecJ_OB)

(454-554)

DHH family (DHH)

(83-228)

DHHA1 domain (DHHA1)

(317-434)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(608-737)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Streptococcus pneumoniae strain D39V

57.047

100

0.573
  recJ Bacillus subtilis subsp. subtilis str. 168

36.585

100

0.384