Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   AM10699_RS25830 Genome accession   NZ_AP026075
Coordinates   5781075..5783102 (+) Length   675 a.a.
NCBI ID   WP_012162938.1    Uniprot ID   B0C484
Organism   Acaryochloris marina MBIC10699     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5776075..5788102
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AM10699_RS25820 (AM10699_50730) - 5777749..5779089 (-) 1341 WP_012162936.1 TldD/PmbA family protein -
  AM10699_RS25825 (AM10699_50740) - 5779164..5780633 (-) 1470 WP_012162937.1 TldD/PmbA family protein -
  AM10699_RS25830 (AM10699_50750) pilB/pilB1 5781075..5783102 (+) 2028 WP_012162938.1 GspE/PulE family protein Machinery gene
  AM10699_RS25835 (AM10699_50760) - 5783148..5784302 (+) 1155 WP_012162939.1 glycosyltransferase -
  AM10699_RS25840 (AM10699_50770) - 5784329..5785504 (-) 1176 WP_012162940.1 glycosyltransferase family 4 protein -
  AM10699_RS25845 (AM10699_50780) - 5785587..5786507 (+) 921 WP_012162941.1 hypothetical protein -
  AM10699_RS25850 (AM10699_50790) - 5786592..5787875 (-) 1284 WP_261890906.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 675 a.a.        Molecular weight: 75420.80 Da        Isoelectric Point: 5.6219

>NTDB_id=95699 AM10699_RS25830 WP_012162938.1 5781075..5783102(+) (pilB/pilB1) [Acaryochloris marina MBIC10699]
MENNSSKRELLVRNNFSPSNEKLIKSGYVDQARLREAIYESRRKGRSLLAILQKMTGKPLPPRLLRQIKRQQLFELKILY
GLDCLDPELTQVDFDEIDRLIRTVIPLDYCRRYQILPLALNADVDPPVVRVGMVNPDHLSAIDDVDYLLRSNGMTFQRVV
LDLDTFQMLIAKFLDAQAKRQATIQANLPVEYGLEDDLDRLQGIEDLSSNDADINLDEALLDENAAPVISLVNKILAKAL
QNGISDIHIEPQEDNLRVRFRKDGLLHNAFQPLPRRIAPALVSRFKIIANLDIAERRLPQDGRILRLFQGRKVDFRVSTL
PSRYGEKVVLRILDNSSMQLGLNHLIDDPTSLAMVREMVDRPFGLILVTGPTGSGKTTTLYSALTERNTPEVNISTVEDP
IEYTLPGITQVQVLREKGMDFASILRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAASAIVRLAEMD
VEPFMVSASLLGVVAQRLVRRLCPDCRIPYRPSPEKLAQFGLMASAKTEITFYKANTSTSDDILATANTEDACPTCSGIG
YSGRCGVYEVMQVTEKLKGLITEGAPTEHIKEVAVEEGMQTLLAYSLKLVEQGATSLEEVERVTFSDSGLKTELKAKRKT
ALNCHVCNAGLLPEWLDCPYCLTPRFAEHHPANQL

Nucleotide


Download         Length: 2028 bp        

>NTDB_id=95699 AM10699_RS25830 WP_012162938.1 5781075..5783102(+) (pilB/pilB1) [Acaryochloris marina MBIC10699]
ATGGAGAATAACTCATCCAAGCGGGAACTGCTCGTTAGAAACAATTTTTCTCCTTCCAATGAAAAATTAATTAAGAGTGG
CTATGTAGATCAAGCACGCTTGCGAGAAGCGATTTATGAAAGTCGGCGGAAGGGACGTTCGCTGCTAGCGATCCTGCAAA
AGATGACGGGGAAACCTTTACCCCCCCGACTTCTTCGACAAATCAAGCGTCAGCAGCTATTTGAGTTAAAAATTCTCTAT
GGACTGGATTGTCTCGATCCTGAGCTGACCCAGGTTGACTTTGACGAAATTGACCGCTTGATTCGCACCGTCATTCCCCT
TGACTATTGTCGTCGCTACCAGATTTTGCCCCTCGCCCTCAATGCCGACGTCGATCCACCAGTTGTCCGTGTGGGCATGG
TCAACCCGGACCACCTCAGTGCCATTGATGATGTTGACTATTTGCTGCGCTCCAATGGGATGACCTTCCAGCGGGTGGTT
CTAGATTTGGATACTTTCCAAATGCTGATCGCCAAGTTCTTGGACGCCCAAGCCAAACGTCAGGCCACCATCCAAGCAAA
CCTGCCCGTTGAATATGGCCTAGAAGATGATCTAGATCGCCTACAGGGGATTGAAGATCTCTCCAGCAATGATGCAGATA
TCAATCTGGATGAAGCCCTACTAGATGAAAATGCAGCGCCAGTCATTTCTTTAGTTAATAAAATATTGGCAAAAGCATTA
CAAAATGGCATTTCTGATATTCATATTGAGCCGCAGGAAGACAATTTGCGCGTTCGTTTTCGCAAAGATGGCTTATTGCA
TAATGCGTTTCAGCCCCTACCTCGGCGCATTGCCCCTGCCCTCGTCTCCCGTTTCAAAATCATCGCCAACTTAGATATCG
CCGAACGGCGTTTACCCCAAGATGGTCGGATTCTCCGGTTATTTCAGGGCCGCAAAGTTGACTTTCGTGTCAGTACCTTA
CCGAGTCGCTATGGCGAAAAGGTCGTTTTGCGGATCTTAGACAACTCATCGATGCAGCTAGGTCTCAACCATCTGATTGA
TGATCCGACCAGCCTCGCAATGGTCCGTGAAATGGTGGATCGGCCTTTTGGGTTGATTTTGGTGACCGGCCCTACTGGTT
CGGGTAAAACGACGACCCTATATTCAGCCTTGACGGAGCGCAATACTCCCGAAGTCAATATCAGTACCGTTGAGGACCCC
ATTGAATATACGCTGCCGGGGATTACCCAGGTACAGGTCTTGCGGGAAAAAGGCATGGACTTTGCCTCCATTCTGCGGGC
CTTTCTACGGCAAGACCCTGATGTCCTACTCGTGGGTGAGACCCGAGACAAGGAAACGGCTAAAACGGCGATTGAAGCCG
CGTTAACGGGACACTTAGTGTTAACAACCCTACACACCAACGATGCAGCCAGTGCCATTGTTCGTTTGGCAGAAATGGAT
GTTGAACCGTTTATGGTCTCAGCGTCCTTATTGGGTGTGGTGGCTCAACGACTCGTCCGCCGCCTCTGTCCAGACTGTCG
AATTCCCTATCGTCCTTCCCCAGAAAAGCTAGCTCAGTTTGGGTTAATGGCTTCGGCCAAAACAGAAATCACCTTTTACA
AGGCCAATACCTCTACGTCTGACGATATTTTGGCAACGGCAAATACAGAGGATGCCTGTCCCACCTGTTCCGGTATCGGC
TATTCCGGGCGATGCGGGGTCTATGAAGTTATGCAGGTGACCGAAAAGTTAAAGGGACTAATTACCGAAGGTGCACCAAC
AGAGCATATCAAGGAAGTGGCTGTTGAAGAAGGGATGCAAACCTTGTTGGCTTATAGTTTGAAGCTGGTTGAGCAAGGCG
CTACATCCTTAGAAGAAGTAGAACGGGTGACCTTCTCTGATAGCGGCTTGAAAACGGAGCTCAAGGCAAAGCGAAAAACA
GCCCTCAACTGTCATGTTTGCAATGCAGGACTCTTACCGGAGTGGTTAGACTGTCCCTACTGCCTAACGCCCAGGTTTGC
AGAGCATCATCCAGCCAATCAACTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(61-178)

Type II/IV secretion system protein (T2SSE)

(234-501)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B0C484

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

59.789

98.37

0.588

Multiple sequence alignment