Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WJM78_RS18870 Genome accession   NZ_CP149129
Coordinates   4077565..4079004 (-) Length   479 a.a.
NCBI ID   WP_339010990.1    Uniprot ID   -
Organism   Aeromonas popoffii strain BE5     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4072565..4084004
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WJM78_RS18840 - 4072839..4073579 (+) 741 WP_339010982.1 KDGP aldolase family protein -
  WJM78_RS18845 - 4073682..4075586 (+) 1905 WP_339010983.1 PTS sugar transporter subunit IIA -
  WJM78_RS18850 npr 4075605..4075877 (-) 273 WP_005331359.1 PTS phosphocarrier protein NPr -
  WJM78_RS18855 rapZ 4075898..4076764 (-) 867 WP_042039092.1 RNase adapter RapZ -
  WJM78_RS18860 ptsN 4076807..4077253 (-) 447 WP_042039095.1 PTS IIA-like nitrogen regulatory protein PtsN -
  WJM78_RS18865 hpf 4077256..4077543 (-) 288 WP_042039097.1 ribosome hibernation promoting factor -
  WJM78_RS18870 rpoN/rpoN1 4077565..4079004 (-) 1440 WP_339010990.1 RNA polymerase factor sigma-54 Machinery gene
  WJM78_RS18875 lptB 4079070..4079795 (-) 726 WP_042039100.1 LPS export ABC transporter ATP-binding protein -
  WJM78_RS18880 lptA 4079799..4080326 (-) 528 WP_339015928.1 lipopolysaccharide transport periplasmic protein LptA -
  WJM78_RS18885 lptC 4080313..4080873 (-) 561 WP_339010992.1 LPS export ABC transporter periplasmic protein LptC -
  WJM78_RS18890 kdsC 4080870..4081424 (-) 555 WP_108537008.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  WJM78_RS18895 - 4081424..4082419 (-) 996 WP_339010994.1 KpsF/GutQ family sugar-phosphate isomerase -
  WJM78_RS18900 mlaF 4082627..4083427 (+) 801 WP_042039109.1 phospholipid ABC transporter ATP-binding protein MlaF -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53807.78 Da        Isoelectric Point: 4.3391

>NTDB_id=956749 WJM78_RS18870 WP_339010990.1 4077565..4079004(-) (rpoN/rpoN1) [Aeromonas popoffii strain BE5]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENDVQETSQEEPIDASQLDSSDAMAQDQMP
DELPVDTTWEDVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDLDAAIALAIIDAIDDSGYLTVSLED
IQAAVSSEDVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLGQYAPELPWLNEAKEVLNEHMDLLGNRDYRTLARK
TKLKECDLKEVLDLIQQLEPRPGNGIIQSDSQYVIPDVTVVKKGPKWVVELNPDSAPRIRVNETYAAMARQARTSQDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=956749 WJM78_RS18870 WP_339010990.1 4077565..4079004(-) (rpoN/rpoN1) [Aeromonas popoffii strain BE5]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACTATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACTCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGATAACAACCCCTTGCTGGAGCAAGAGGAGAATGACG
TGCAGGAGACCAGCCAGGAAGAGCCAATCGATGCCAGTCAGCTCGACTCCAGCGATGCCATGGCCCAGGATCAGATGCCG
GATGAGTTGCCGGTGGACACCACCTGGGAAGATGTCTACTCGGCCGGCACAGCCCAGAGCGCAGGCCCCATCCACGATGG
GGAAGACAGCGTCTATCAGGGTGAAACCACCGAGAACCTGCAGGACTATCTACTGTGGCAGATGCGGCTCACGCCGTTTA
GCGATCTGGATGCCGCCATTGCCCTCGCCATCATAGATGCCATCGACGATTCCGGTTATCTGACCGTCTCCCTCGAAGAT
ATACAGGCGGCGGTGAGCAGCGAGGATGTCGAGGTGGAACTCGACGAGGTTGAGGCCGTGCTCAAGCGGGTGCAGCATTT
CGATCCAGTCGGTATCGCCGCTCGCTCGGTGCAGGAATGTCTGTTGATCCAGCTTGGTCAATATGCCCCCGAACTGCCCT
GGCTCAATGAGGCCAAGGAGGTGCTCAACGAGCACATGGACTTGCTCGGCAATCGTGATTATCGGACCCTGGCACGCAAG
ACCAAGCTCAAGGAGTGCGATCTCAAGGAGGTGCTGGATCTGATTCAGCAGCTGGAGCCCAGACCGGGCAATGGCATCAT
ACAGAGCGACTCCCAGTACGTGATCCCGGATGTGACTGTGGTCAAGAAGGGGCCCAAATGGGTGGTAGAGCTCAATCCCG
ATTCTGCTCCACGCATCCGGGTCAATGAGACCTATGCCGCGATGGCGCGCCAGGCGCGCACCAGCCAGGACACCCAGTTC
ATCCGCTCCCATCTGCAAGAGGCCAAGTGGTTTATCAAGAGTCTGGAGAGTCGCAATGACACCCTGCTCAAGGTGGCCAG
CTGTATTGTCGAGCAGCAACAGGGCTTTTTCGAGTATGGGGAAGAAGCCATGAAACCCATGGTGCTCAACGACATCGCCG
AAGCGGTGGAGATGCATGAGTCTACCATTTCCCGGGTAACGACCCAGAAATACATGCATACGCCCCGTGGCATCTTCGAA
CTGAAGTTTTTTTTCTCCAGTCATGTGAACACCGAAGGAGGAGGAGAGTGCTCATCGACCGCAATTCGTGCGCTGATCAA
GAAGTTGGTGACGGCAGAGAATCCCGCCAAGCCACTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.972

100

0.489