Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACIFYW_RS02355 Genome accession   NZ_CP173026
Coordinates   491940..492344 (-) Length   134 a.a.
NCBI ID   WP_057551631.1    Uniprot ID   -
Organism   Vibrio cholerae strain SPH 24-01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 486940..497344
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIFYW_RS02335 (ACIFYW_02335) coaE 487438..488046 (-) 609 WP_000011553.1 dephospho-CoA kinase -
  ACIFYW_RS02340 (ACIFYW_02340) pilD 488043..488918 (-) 876 WP_267774830.1 A24 family peptidase Machinery gene
  ACIFYW_RS02345 (ACIFYW_02345) pilC 488976..490202 (-) 1227 WP_248431770.1 type II secretion system F family protein Machinery gene
  ACIFYW_RS02350 (ACIFYW_02350) pilB 490245..491933 (-) 1689 WP_404272710.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACIFYW_RS02355 (ACIFYW_02355) pilA 491940..492344 (-) 405 WP_057551631.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  ACIFYW_RS02360 (ACIFYW_02360) nadC 492589..493479 (-) 891 WP_000665278.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACIFYW_RS02365 (ACIFYW_02365) ampD 493777..494322 (+) 546 WP_001959072.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACIFYW_RS02370 (ACIFYW_02370) - 494477..495865 (-) 1389 WP_001959226.1 IS4-like element ISVa18 family transposase -
  ACIFYW_RS02375 (ACIFYW_02375) fldB 496004..496522 (-) 519 WP_000690112.1 flavodoxin FldB -

Sequence

Protein


Download         Length: 134 a.a.        Molecular weight: 13586.69 Da        Isoelectric Point: 9.0173

>NTDB_id=956749 ACIFYW_RS02355 WP_057551631.1 491940..492344(-) (pilA) [Vibrio cholerae strain SPH 24-01]
MKAYKNKLQQGFTLIELMIVVAVIGVLAAIAVPQYQKYTQKAALATALATATALKTNYEDYVSMSGAAPTSATDIGATSF
SIGTISVTSGAINVAINKGPGTGSSVILTRTGLLGGWGCQISGAQAEVTINGCP

Nucleotide


Download         Length: 405 bp        

>NTDB_id=956749 ACIFYW_RS02355 WP_057551631.1 491940..492344(-) (pilA) [Vibrio cholerae strain SPH 24-01]
ATGAAAGCGTATAAAAATAAACTACAGCAAGGTTTTACCTTAATTGAATTGATGATTGTAGTGGCAGTGATTGGTGTGTT
GGCAGCGATTGCGGTGCCTCAATATCAGAAATATACACAAAAAGCAGCTCTTGCTACTGCTTTAGCTACAGCAACTGCAT
TAAAAACAAATTATGAAGACTATGTATCTATGAGTGGTGCAGCTCCCACATCTGCAACAGATATTGGAGCAACCAGCTTC
AGTATAGGAACTATTTCAGTGACTAGTGGTGCTATAAACGTTGCTATAAATAAGGGACCAGGCACTGGCTCATCTGTAAT
TTTAACTAGAACAGGGCTTCTTGGTGGCTGGGGATGCCAAATCTCCGGAGCTCAAGCTGAAGTAACAATTAACGGTTGCC
CATAG

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(41-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

43.243

100

0.478
  pilA Vibrio cholerae strain A1552

43.243

100

0.478
  pilA Vibrio cholerae C6706

43.243

100

0.478
  pilA Vibrio parahaemolyticus RIMD 2210633

39.189

100

0.433
  pilA Pseudomonas aeruginosa PAK

38.514

100

0.425
  pilA Vibrio campbellii strain DS40M4

37.013

100

0.425
  pilA Acinetobacter baumannii strain A118

38.519

100

0.388
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.462

97.015

0.373
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.2

93.284

0.366