Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACIFYW_RS01825 Genome accession   NZ_CP173026
Coordinates   375538..377001 (-) Length   487 a.a.
NCBI ID   WP_000804830.1    Uniprot ID   Q9KP49
Organism   Vibrio cholerae strain SPH 24-01     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 370538..382001
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIFYW_RS01795 (ACIFYW_01795) pmbA 370680..372023 (+) 1344 WP_404272702.1 metalloprotease PmbA -
  ACIFYW_RS01800 (ACIFYW_01800) mgtE 372095..373456 (-) 1362 WP_000840452.1 magnesium transporter -
  ACIFYW_RS01805 (ACIFYW_01805) - 373617..373895 (-) 279 WP_001135899.1 HPr family phosphocarrier protein -
  ACIFYW_RS01810 (ACIFYW_01810) rapZ 373904..374764 (-) 861 WP_001237196.1 RNase adapter RapZ -
  ACIFYW_RS01815 (ACIFYW_01815) ptsN 374777..375223 (-) 447 WP_001177163.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACIFYW_RS01820 (ACIFYW_01820) hpf 375226..375513 (-) 288 WP_404272703.1 ribosome hibernation promoting factor -
  ACIFYW_RS01825 (ACIFYW_01825) rpoN/rpoN1 375538..377001 (-) 1464 WP_000804830.1 RNA polymerase factor sigma-54 Machinery gene
  ACIFYW_RS01830 (ACIFYW_01830) lptB 377057..377782 (-) 726 WP_000996178.1 LPS export ABC transporter ATP-binding protein -
  ACIFYW_RS01835 (ACIFYW_01835) lptA 377785..378279 (-) 495 WP_000830444.1 lipopolysaccharide transport periplasmic protein LptA -
  ACIFYW_RS01840 (ACIFYW_01840) lptC 378260..378823 (-) 564 WP_000059348.1 LPS export ABC transporter periplasmic protein LptC -
  ACIFYW_RS01845 (ACIFYW_01845) kdsC 378820..379377 (-) 558 WP_000099768.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ACIFYW_RS01850 (ACIFYW_01850) kdsD 379377..380351 (-) 975 WP_001120614.1 arabinose-5-phosphate isomerase KdsD -
  ACIFYW_RS01855 (ACIFYW_01855) - 380407..381369 (-) 963 WP_000904329.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 487 a.a.        Molecular weight: 55438.47 Da        Isoelectric Point: 4.2824

>NTDB_id=956745 ACIFYW_RS01825 WP_000804830.1 375538..377001(-) (rpoN/rpoN1) [Vibrio cholerae strain SPH 24-01]
MKPSLQLKLGQQLAMTPQLQQAIRLLQLSTLDLQQEIQEALESNPLLEVEENQDEAPSLDSVRMTEESPREPEELYEPEP
QDSSDLIEKSEISAELEMDTTWDEVYSANTGSTGLALDDDAPIYQGETTQTLQDYLHWQLDLTPFSDVDRTIAVALIDAI
DDYGYLTVSLEEIQESLRSDDIELDEIEAVRKRIQQFDPFGVASLNLQDCLLLQLTTYPCDTPWLEEARLLLSQYIDDLG
NRDYKTILKETKLKEEDLREILQLIQQLDPRPGSRIAQDHAEYVIPDVSVYKEQGRWLVTINPDSVPKLKINQQYADLMR
GNNAESNYIRTNLQEAKWLIKSLESRNETLLKVAKCIVEHQHDFFEYGEEAMKPMVLNDVAMAVEMHESTISRVTTQKYM
HTPRGIFELKYFFSSHVSTDNGGECSSTAIRALIKKLVAAENPAKPLSDSKIATLLADQGIQVARRTIAKYRESLGIAPS
SQRKRLL

Nucleotide


Download         Length: 1464 bp        

>NTDB_id=956745 ACIFYW_RS01825 WP_000804830.1 375538..377001(-) (rpoN/rpoN1) [Vibrio cholerae strain SPH 24-01]
ATGAAACCGTCATTACAGCTCAAGCTAGGTCAACAGTTAGCCATGACTCCGCAGCTCCAGCAGGCGATTCGTCTGTTGCA
GCTTTCGACGCTGGATCTGCAGCAAGAAATTCAGGAAGCATTGGAATCTAACCCCTTGTTAGAGGTGGAAGAGAACCAAG
ACGAAGCGCCATCGCTTGATAGCGTACGTATGACGGAAGAAAGCCCGCGCGAGCCAGAAGAACTCTACGAACCTGAACCG
CAGGATAGCTCGGATCTGATTGAAAAATCGGAAATCAGCGCTGAATTAGAGATGGATACCACTTGGGATGAAGTCTACAG
CGCCAATACTGGCAGTACAGGACTGGCACTCGATGATGATGCCCCTATCTACCAAGGCGAAACTACCCAAACCCTGCAAG
ATTATCTTCACTGGCAACTCGACTTAACTCCCTTTAGCGATGTGGATCGCACTATAGCCGTTGCACTTATCGATGCTATC
GACGATTACGGTTATTTAACCGTCTCACTAGAGGAGATCCAAGAGAGCCTGCGCAGTGATGACATTGAGTTGGATGAGAT
TGAAGCGGTACGTAAACGCATTCAGCAGTTTGACCCCTTTGGTGTGGCATCGCTTAATCTGCAAGACTGCTTGTTGCTGC
AACTCACCACCTATCCCTGTGATACTCCTTGGCTGGAAGAAGCGCGTTTACTGCTCTCACAGTACATCGATGATTTAGGG
AATCGCGATTACAAAACCATTCTGAAAGAGACTAAGCTCAAAGAAGAGGACTTGCGCGAGATCCTGCAACTCATCCAACA
GCTCGATCCTCGCCCCGGCAGTCGAATTGCCCAAGATCACGCTGAATACGTTATACCTGACGTGTCAGTCTATAAAGAAC
AAGGTCGATGGCTGGTCACCATCAACCCAGATAGTGTGCCTAAACTGAAGATCAATCAGCAGTATGCCGACCTGATGCGC
GGTAATAATGCGGAGAGCAACTACATCCGTACCAATTTGCAAGAGGCAAAATGGCTGATCAAAAGTTTAGAAAGTAGAAA
CGAGACACTGCTCAAAGTTGCCAAGTGCATAGTTGAACACCAACACGATTTCTTCGAGTATGGAGAAGAGGCGATGAAGC
CGATGGTGCTCAACGATGTGGCGATGGCGGTGGAAATGCATGAATCGACAATTTCGCGTGTCACCACGCAGAAATACATG
CATACCCCGCGCGGCATTTTTGAGTTGAAGTACTTTTTCTCCAGCCACGTCAGTACTGACAATGGCGGAGAGTGCTCGTC
CACTGCGATCAGGGCGCTGATCAAAAAACTGGTGGCGGCAGAGAATCCTGCCAAACCACTGAGTGACAGCAAGATCGCAA
CCCTACTCGCTGACCAAGGCATCCAAGTCGCGCGGCGAACCATCGCCAAATATCGTGAATCACTTGGCATCGCCCCATCA
AGTCAGCGCAAACGCCTGCTATAG

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-49)

Sigma-54, DNA binding domain (Sigma54_DBD)

(327-485)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(128-315)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9KP49

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.533

100

0.46