Detailed information NTG0956604

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: LLI816_RS05560
• Genome accession: NZ_CP149095
• Coordinates: 1066661..1067983 (-)
• Length: 440 a.a.
• NCBI ID: WP_058221332.1
• Uniprot ID: -
• Organism: Lactococcus lactis subsp. lactis strain I8-16
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1061661..1072983

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLI816_RS05530 (LLI816_05530)	-	1062804..1063772 (+)	969	WP_003130649.1	PhoH family protein	-
LLI816_RS05535 (LLI816_05535)	-	1063830..1064309 (+)	480	WP_003130650.1	NUDIX hydrolase	-
LLI816_RS05540 (LLI816_05540)	ybeY	1064461..1064949 (+)	489	WP_003130652.1	rRNA maturation RNase YbeY	-
LLI816_RS05545 (LLI816_05545)	-	1064933..1065388 (+)	456	WP_003130653.1	diacylglycerol kinase family protein	-
LLI816_RS05550 (LLI816_05550)	-	1065402..1065980 (+)	579	WP_003130655.1	nucleotidyltransferase family protein	-
LLI816_RS05555 (LLI816_05555)	comFC	1066014..1066418 (-)	405	WP_003130657.1	ComF family protein	Machinery gene
LLI816_RS05560 (LLI816_05560)	comFA	1066661..1067983 (-)	1323	WP_058221332.1	DEAD/DEAH box helicase	Machinery gene
LLI816_RS05565 (LLI816_05565)	-	1068040..1068669 (+)	630	WP_003130660.1	YigZ family protein	-
LLI816_RS05570 (LLI816_05570)	-	1068803..1069300 (+)	498	WP_003130662.1	VanZ family protein	-
LLI816_RS05575 (LLI816_05575)	-	1069348..1070367 (+)	1020	WP_003130663.1	DUF475 domain-containing protein	-
LLI816_RS05580 (LLI816_05580)	-	1070483..1071229 (+)	747	WP_003130666.1	bifunctional metallophosphatase/5'-nucleotidase	-
LLI816_RS05585 (LLI816_05585)	glyQ	1071514..1072467 (+)	954	WP_003130667.1	glycine--tRNA ligase subunit alpha	-

Sequence

Protein

• Length: 440 a.a.
• Molecular weight: 50201.71 Da
• Isoelectric Point: 10.1065

>NTDB_id=956604 LLI816_RS05560 WP_058221332.1 1066661..1067983(-) (comFA) [Lactococcus lactis subsp. lactis strain I8-16]
MSTNQEKLFGRLLLKNDILQLIKNTDKISVSKIFSNFLLETKVNPILGMTSISSNKIKCNRCGTVHIKNSVKLPIGVFYC
PSCIQLGRVRSDEFLYFLPQKNFPKKSYINWSGKLTENQKSISNALCQEINSHQQIIVQAVTGAGKTEMIYQVIEQILES
GGVVGLASPRIDVCLELHQRLSRDFSCKIPLLYHDGDSYFRAPLIIMTSHQLLRFKEAFDLLIIDEVDAFPFRDNEMLYV
AAEKARKIEGNLIYLTATSTDKLEKDIKKQKLYPLFLPRRFHNFPLVVPKFFWKNKFDKKLIEQRNSGFPLLIFAAEIEF
GQEFAKQLQLKFPKEKIASVASTTKDRLEIVKAFRNKEITILIATSILERGVTFPNVDVFVINSEHPNFTKSALIQMAGR
VGRSPERPTGLVSFFHYGKSKAMCQAVREIKKMNQLGGFS

Nucleotide

• Length: 1323 bp

>NTDB_id=956604 LLI816_RS05560 WP_058221332.1 1066661..1067983(-) (comFA) [Lactococcus lactis subsp. lactis strain I8-16]
ATGAGCACTAATCAAGAAAAGTTATTTGGCCGTTTATTATTAAAAAATGATATTTTGCAACTTATAAAAAATACTGACAA
GATTTCTGTCAGTAAAATTTTTAGTAATTTTTTGTTAGAAACGAAGGTGAATCCAATTTTGGGAATGACTTCAATTTCTT
CCAATAAAATAAAATGCAACCGTTGTGGGACTGTTCATATAAAAAATTCTGTCAAACTTCCAATTGGTGTATTTTACTGT
CCAAGTTGTATTCAATTAGGTCGAGTCCGCTCCGATGAATTCTTGTACTTTCTGCCACAAAAGAATTTCCCAAAGAAATC
ATATATAAACTGGTCTGGAAAACTGACAGAGAATCAAAAATCAATTTCAAATGCCCTTTGTCAGGAAATTAATTCTCATC
AGCAAATAATTGTCCAAGCTGTGACTGGAGCTGGAAAAACTGAAATGATTTATCAAGTCATTGAGCAAATTTTAGAAAGC
GGTGGGGTTGTTGGTCTAGCTAGTCCAAGAATTGATGTTTGTCTTGAACTTCATCAGCGATTATCACGTGATTTTTCCTG
TAAGATTCCACTCTTATATCATGATGGCGACAGCTATTTCCGAGCTCCATTAATAATAATGACCAGTCATCAGCTTTTAC
GTTTCAAGGAAGCTTTTGATTTGCTGATTATTGATGAAGTTGATGCCTTTCCCTTTAGAGATAATGAAATGCTTTATGTT
GCGGCAGAAAAAGCAAGAAAAATAGAAGGAAATTTAATATATTTGACCGCAACTTCTACTGACAAACTTGAAAAAGATAT
AAAAAAGCAAAAACTCTATCCTTTGTTTCTCCCGCGTCGTTTTCACAATTTCCCTTTAGTGGTGCCTAAATTTTTTTGGA
AAAATAAATTTGATAAGAAATTAATTGAGCAAAGAAATAGTGGCTTTCCTCTTCTTATTTTTGCTGCTGAAATTGAATTT
GGACAAGAATTTGCAAAACAACTACAATTGAAATTTCCTAAAGAAAAAATTGCTTCCGTTGCTTCAACAACAAAAGATAG
ATTGGAAATTGTTAAAGCTTTTAGAAATAAAGAAATTACTATTTTAATAGCGACATCAATTCTTGAACGGGGAGTCACTT
TTCCAAATGTTGATGTTTTTGTCATCAACAGTGAGCACCCAAACTTCACTAAATCTGCACTGATACAAATGGCTGGACGT
GTGGGTCGTAGTCCTGAACGTCCAACAGGCTTAGTTAGTTTTTTTCATTACGGAAAATCCAAAGCAATGTGTCAGGCAGT
TAGAGAAATCAAAAAAATGAACCAACTGGGAGGTTTTTCATGA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (114-233)

• Helicase conserved C-terminal domain (Helicase_C) (300-403)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Lactococcus lactis subsp. cremoris KW2	74.5	90.909	0.677
comFA/cflA	Streptococcus mitis SK321	52.01	90.455	0.47
comFA/cflA	Streptococcus pneumoniae Rx1	52.01	90.455	0.47
comFA/cflA	Streptococcus pneumoniae D39	52.01	90.455	0.47
comFA/cflA	Streptococcus pneumoniae R6	52.01	90.455	0.47
comFA/cflA	Streptococcus pneumoniae TIGR4	52.01	90.455	0.47
comFA/cflA	Streptococcus mitis NCTC 12261	51.256	90.455	0.464
comFA	Bacillus subtilis subsp. subtilis str. 168	38.018	98.636	0.375