Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACFACP_RS23885 Genome accession   NZ_CP172860
Coordinates   5106514..5108007 (+) Length   497 a.a.
NCBI ID   WP_034076736.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 238627E_ID003     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5101514..5113007
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFACP_RS23855 (ACFACP_23855) - 5101726..5102535 (-) 810 WP_003094343.1 ABC transporter ATP-binding protein -
  ACFACP_RS23860 (ACFACP_23860) kdsD 5102949..5103929 (+) 981 WP_003094347.1 arabinose-5-phosphate isomerase KdsD -
  ACFACP_RS23865 (ACFACP_23865) - 5103929..5104468 (+) 540 WP_003112742.1 HAD family hydrolase -
  ACFACP_RS23870 (ACFACP_23870) lptC 5104477..5105049 (+) 573 WP_003094353.1 LPS export ABC transporter periplasmic protein LptC -
  ACFACP_RS23875 (ACFACP_23875) lptA 5105036..5105563 (+) 528 WP_003120916.1 lipopolysaccharide transport periplasmic protein LptA -
  ACFACP_RS23880 (ACFACP_23880) lptB 5105563..5106288 (+) 726 WP_003094355.1 LPS export ABC transporter ATP-binding protein -
  ACFACP_RS23885 (ACFACP_23885) rpoN/rpoN1 5106514..5108007 (+) 1494 WP_034076736.1 RNA polymerase factor sigma-54 Machinery gene
  ACFACP_RS23890 (ACFACP_23890) hpf 5108085..5108393 (+) 309 WP_003094359.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ACFACP_RS23895 (ACFACP_23895) ptsN 5108407..5108871 (+) 465 WP_003098853.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACFACP_RS23900 (ACFACP_23900) rapZ 5108873..5109733 (+) 861 WP_003094361.1 RNase adapter RapZ -
  ACFACP_RS23905 (ACFACP_23905) - 5109763..5110035 (+) 273 WP_003094362.1 HPr family phosphocarrier protein -
  ACFACP_RS23910 (ACFACP_23910) - 5110132..5111064 (-) 933 WP_003123303.1 ZIP family metal transporter -
  ACFACP_RS23915 (ACFACP_23915) - 5111083..5111694 (-) 612 WP_031643275.1 superoxide dismutase -
  ACFACP_RS23920 (ACFACP_23920) - 5111707..5112156 (-) 450 WP_003094369.1 hypothetical protein -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 56054.22 Da        Isoelectric Point: 4.4327

>NTDB_id=955756 ACFACP_RS23885 WP_034076736.1 5106514..5108007(+) (rpoN/rpoN1) [Pseudomonas aeruginosa strain 238627E_ID003]
MKPSLVLKMGQQLTMTPQLQQAIRLLQLSTLDLQQEIQEALESNPMLERQEDGDDFDNSDPLADGAEQAASAPQESPIQE
SATPSVESLDDDQWSERIPSELPVDTAWEDIYQTSASSLPSNDDDEWDFTARTSSGESLHSHLLWQVNLAPMSDTDRMIA
VTIIDSINNDGYLEESLEEILAAIDPELDVELDEVEVVLRRIQQLEPAGIGARNLRECLLLQLRQLPSTTPWLNEALRLV
SDYLDLLGGRDYSQLMRRMKLKEDELRQVIELIQCLHPRPGSQIESSEAEYIVPDVIVRKDNERWLVELNQEAMPRLRVN
ATYAGMVRRADSSADNTFMRNQLQEARWFIKSLQSRNETLMKVATQIVEHQRGFLDYGEEAMKPLVLHDIAEAVGMHEST
ISRVTTQKYMHTPRGIFELKYFFSSHVSTAEGGECSSTAIRAIIKKLVAAENAKKPLSDSKIAGLLEAQGIQVARRTVAK
YRESLGIAPSSERKRLV

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=955756 ACFACP_RS23885 WP_034076736.1 5106514..5108007(+) (rpoN/rpoN1) [Pseudomonas aeruginosa strain 238627E_ID003]
ATGAAACCATCGCTAGTCCTCAAGATGGGCCAGCAGCTGACGATGACGCCGCAGCTGCAACAGGCCATACGCCTCCTCCA
GCTATCCACCCTGGACCTCCAGCAGGAAATCCAGGAAGCGCTGGAATCCAATCCCATGCTCGAACGCCAGGAAGACGGCG
ACGATTTCGACAACAGCGACCCTCTCGCCGATGGCGCCGAACAGGCGGCCAGCGCGCCCCAGGAGAGCCCTATCCAGGAA
AGCGCCACGCCCAGCGTCGAAAGCCTCGACGACGACCAGTGGAGCGAACGCATACCCAGCGAGTTGCCGGTGGATACCGC
CTGGGAAGACATCTACCAGACCAGCGCCAGCAGCCTGCCCAGCAACGACGATGACGAATGGGACTTCACCGCGCGCACCA
GCAGCGGCGAGAGCCTGCACAGCCACCTGCTCTGGCAGGTCAACCTGGCACCGATGTCGGACACCGATCGGATGATCGCG
GTGACCATCATCGATAGCATCAACAACGATGGCTACCTCGAAGAGTCCCTCGAAGAAATCCTCGCCGCCATCGATCCGGA
ACTGGACGTCGAGCTCGACGAAGTGGAAGTCGTGCTGCGCCGCATCCAGCAACTGGAACCCGCCGGCATCGGCGCCCGCA
ACCTGCGCGAGTGCCTGCTGCTGCAACTGCGCCAGTTGCCGTCCACCACGCCCTGGCTGAACGAAGCGCTGCGCCTGGTA
TCCGACTACCTCGACCTGCTCGGCGGCCGCGACTACAGCCAATTGATGCGGCGCATGAAGCTCAAGGAAGACGAACTGCG
CCAGGTCATCGAGCTGATCCAGTGCCTGCATCCCCGGCCCGGTTCGCAGATCGAATCGAGCGAAGCCGAATACATCGTCC
CCGACGTCATCGTGCGCAAGGACAACGAGCGCTGGCTGGTCGAGCTGAACCAGGAGGCGATGCCACGCCTGCGCGTCAAC
GCCACCTACGCCGGCATGGTCCGCCGCGCCGACAGCAGCGCCGACAATACCTTCATGCGCAACCAGTTGCAGGAAGCCCG
CTGGTTCATCAAGAGCCTGCAGAGCCGCAACGAAACGCTGATGAAGGTCGCCACCCAGATCGTCGAGCACCAGCGCGGTT
TCCTCGACTACGGCGAAGAAGCCATGAAGCCGCTGGTCCTGCACGACATCGCCGAAGCGGTGGGCATGCACGAATCGACC
ATTTCGCGGGTCACCACGCAGAAATACATGCATACGCCGCGCGGCATTTTCGAGCTGAAATATTTCTTTTCAAGTCACGT
CAGTACCGCCGAAGGCGGGGAATGCTCGTCCACCGCGATCCGCGCGATCATCAAGAAACTGGTGGCCGCGGAAAATGCGA
AAAAGCCATTGAGCGACAGCAAGATCGCTGGTTTACTGGAGGCACAGGGCATTCAAGTGGCTCGCCGCACCGTCGCCAAA
TACCGCGAATCCCTGGGCATAGCCCCTTCGAGCGAGCGCAAGCGACTGGTGTGA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(337-495)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(129-323)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

46.457

100

0.475