Detailed information NTG0954968

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: ACFELM_RS27565
• Genome accession: NZ_CP172852
• Coordinates: 5907969..5910188 (+)
• Length: 739 a.a.
• NCBI ID: WP_003146407.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain 238636E_ID015
• Function: DNA puliing through the inner membrane (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5902969..5915188

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACFELM_RS27545 (ACFELM_27545)	-	5903944..5905212 (-)	1269	WP_003103278.1	malic enzyme	-
ACFELM_RS27550 (ACFELM_27550)	-	5905315..5906754 (-)	1440	WP_003095844.1	M48 family metalloprotease	-
ACFELM_RS27555 (ACFELM_27555)	-	5906751..5907518 (-)	768	WP_031633251.1	thermonuclease family protein	-
ACFELM_RS27560 (ACFELM_27560)	rpmE	5907578..5907793 (-)	216	WP_003095846.1	50S ribosomal protein L31	-
ACFELM_RS27565 (ACFELM_27565)	priA	5907969..5910188 (+)	2220	WP_003146407.1	primosomal protein N'	Machinery gene
ACFELM_RS27570 (ACFELM_27570)	argS	5910438..5912201 (+)	1764	WP_003110892.1	arginine--tRNA ligase	-
ACFELM_RS27575 (ACFELM_27575)	-	5912236..5912931 (+)	696	WP_003457586.1	SPOR domain-containing protein	-
ACFELM_RS27580 (ACFELM_27580)	hslV	5913050..5913583 (+)	534	WP_003095852.1	ATP-dependent protease subunit HslV	-
ACFELM_RS27585 (ACFELM_27585)	hslU	5913612..5914955 (+)	1344	WP_003095854.1	ATP-dependent protease ATPase subunit HslU	-

Sequence

Protein

• Length: 739 a.a.
• Molecular weight: 82151.68 Da
• Isoelectric Point: 8.9189

>NTDB_id=954968 ACFELM_RS27565 WP_003146407.1 5907969..5910188(+) (priA) [Pseudomonas aeruginosa strain 238636E_ID015]
MSDLILRLALPSPLRRLFDYRAPRGIPRSALQPGIRLLLPFGRRELVGVLIEVTDRSEVPEEKLKPALRVLDAKPPMPAH
LLELCRWTAQYYQHSLGDTLSWALPNLLRQGEPAEARQQRFWHATAQSSLDDPRLARAPRQRQALAILKQHPHGVSHELL
NQLQINKDSLDLLKEKGLVELEVRRHSTPPREGGWLAQAELPLNPEQRAAFEAVRASHGGFHCFLLAGVTGSGKTEVYLQ
LIRETLAAGRQALVLIPEINLGPQTLARFERRFNARIALLHSALTDRERLDAWLAARDGEADIVIGTRSALFTPLKRPGL
IIVDEEHDASYKQQDGLRYHARDLALVRARLENVPILLGSATPALESLHNAQAGRYGLLRLTQRAGGAHPPKFIRLDVKS
MPLDAGLSRPLQQAIGDTLAAGQQVLVFLNRRGFAPTLLCHDCGWISQCPRCDARMTVHQGSGELRCHHCDHRQRPPMNC
PQCGKLDLRPVGAGTERAEERLRILFPNHPVLRIDRDSTSRKHAMRDLFATINSGEPCILVGTQMLAKGHHFPRVTLVAI
LDADGGLFSADFRASERMAQQIVQVAGRAGRAEEPGRVLIQTHLADHPLLVQLTEDGYFAFAEQALSERRAAGLPPFAHL
ALLRAEAHKPGQAEAFLDSACSAAEQLLEQMGGPEVELLGPVPAPMERRAGKHRAQLLLQCMSRAPLHRLLTPWLQSLEQ
LPGGRQVRWSLDIDPIDLF

Nucleotide

• Length: 2220 bp

>NTDB_id=954968 ACFELM_RS27565 WP_003146407.1 5907969..5910188(+) (priA) [Pseudomonas aeruginosa strain 238636E_ID015]
GTGTCAGACCTGATCCTACGCCTAGCCCTGCCTTCGCCACTCCGCCGCCTGTTCGACTACCGCGCGCCCCGCGGCATTCC
GCGCAGCGCCCTACAGCCGGGGATTCGCCTGCTGCTGCCGTTCGGGCGCCGGGAGCTGGTTGGCGTTCTGATAGAGGTGA
CGGACCGTTCCGAAGTACCCGAAGAAAAGCTCAAGCCTGCCCTCAGGGTACTCGACGCCAAGCCGCCGATGCCGGCGCAC
CTGCTCGAACTCTGTCGCTGGACCGCCCAGTACTACCAGCACAGCCTCGGCGACACCCTGTCCTGGGCGCTGCCCAACCT
GTTGCGCCAGGGCGAGCCGGCCGAGGCGCGCCAGCAGCGCTTCTGGCATGCCACCGCGCAATCCAGCCTCGACGATCCGC
GCCTGGCCCGGGCGCCGCGCCAGCGCCAGGCCCTGGCGATTCTCAAGCAGCATCCGCACGGGGTGTCCCATGAGTTGCTG
AACCAGTTGCAGATAAACAAGGACAGCCTCGACCTGCTCAAGGAAAAAGGCCTGGTCGAACTGGAGGTGCGGCGCCACAG
CACCCCGCCGCGCGAAGGCGGCTGGCTGGCCCAGGCGGAGTTGCCGCTGAACCCCGAGCAGCGCGCCGCCTTCGAGGCGG
TTCGCGCCAGCCACGGCGGTTTCCATTGCTTCCTGCTGGCCGGGGTCACCGGCAGCGGCAAGACCGAGGTCTACCTGCAA
CTGATCCGCGAAACCCTGGCCGCAGGCCGCCAGGCCCTGGTGCTGATTCCCGAGATCAACCTGGGGCCGCAGACCCTGGC
GCGTTTCGAACGCCGCTTCAACGCGCGCATCGCCCTGCTGCATTCGGCCCTGACCGACCGCGAGCGCCTCGACGCCTGGC
TCGCCGCCCGGGATGGCGAGGCCGACATCGTCATCGGCACCCGCTCGGCACTGTTCACGCCGCTGAAGCGACCGGGACTG
ATCATCGTCGACGAGGAACACGACGCCTCCTATAAACAGCAGGACGGCCTGCGCTACCACGCCCGCGACCTGGCACTGGT
ACGCGCGCGCCTGGAGAATGTGCCGATCCTGCTCGGTTCGGCCACGCCCGCCCTGGAAAGCCTGCACAACGCCCAGGCCG
GCCGCTACGGCCTGCTGCGCCTGACCCAGCGCGCCGGTGGCGCGCACCCGCCGAAGTTCATCCGCCTCGACGTGAAGAGC
ATGCCGCTCGACGCCGGCCTTTCCCGTCCGCTGCAACAGGCCATCGGCGATACCCTGGCGGCCGGCCAGCAGGTCCTGGT
ATTCCTCAATCGCCGCGGCTTCGCTCCGACCCTGCTTTGCCACGACTGCGGCTGGATCAGCCAATGCCCGCGCTGCGACG
CGCGGATGACCGTGCACCAGGGCAGCGGCGAGTTGCGCTGCCACCACTGCGACCACCGCCAGCGGCCGCCGATGAACTGC
CCGCAATGCGGCAAGCTCGACCTGCGCCCGGTAGGCGCCGGCACCGAGCGCGCCGAGGAACGCCTGCGGATTCTCTTCCC
GAACCATCCGGTGCTGCGCATCGACCGCGACAGCACCTCGCGCAAGCACGCCATGCGCGACCTCTTCGCCACCATCAACA
GCGGTGAGCCATGCATCCTGGTCGGCACCCAGATGCTCGCCAAGGGGCACCACTTCCCACGGGTCACCCTGGTGGCGATC
CTCGATGCCGATGGCGGCCTGTTTTCCGCCGACTTCCGCGCCAGCGAACGCATGGCCCAGCAGATCGTCCAGGTCGCCGG
CCGCGCAGGACGCGCCGAGGAGCCCGGACGGGTACTGATCCAGACCCACCTGGCCGATCACCCGCTGCTGGTCCAGCTCA
CCGAGGACGGCTACTTCGCCTTTGCCGAACAGGCCCTCAGCGAACGCCGCGCCGCCGGCCTGCCGCCCTTCGCCCACCTC
GCCCTGCTGCGCGCCGAAGCGCACAAGCCGGGCCAGGCGGAAGCCTTCCTCGACAGCGCCTGCAGTGCCGCCGAGCAATT
GCTCGAGCAGATGGGCGGACCGGAGGTGGAACTGCTCGGGCCGGTGCCCGCGCCCATGGAGCGGCGTGCCGGCAAGCACC
GTGCGCAACTGCTGCTGCAGTGCATGAGCCGGGCGCCGCTGCATCGCCTGCTGACGCCCTGGCTGCAGAGCCTCGAACAG
TTGCCCGGCGGGCGCCAGGTGCGCTGGTCGCTCGATATCGATCCGATCGACCTGTTCTGA

Domains

Predicted by InterProScan.

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (449-475)

• Helicase conserved C-terminal domain (Helicase_C) (522-592)

• DEAD/DEAH box helicase (DEAD) (207-367)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (9-105)

• Primosomal protein N C-terminal domain (PriA_C) (636-735)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Acinetobacter baumannii D1279779	47.613	99.188	0.472