Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACFELM_RS04845 Genome accession   NZ_CP172852
Coordinates   1020416..1021909 (-) Length   497 a.a.
NCBI ID   WP_003094357.1    Uniprot ID   A0A0H2ZGR9
Organism   Pseudomonas aeruginosa strain 238636E_ID015     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1015416..1026909
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFELM_RS04810 (ACFELM_04810) - 1016267..1016716 (+) 450 WP_003094369.1 hypothetical protein -
  ACFELM_RS04815 (ACFELM_04815) - 1016729..1017340 (+) 612 WP_003098862.1 superoxide dismutase -
  ACFELM_RS04820 (ACFELM_04820) - 1017359..1018291 (+) 933 WP_003123303.1 ZIP family metal transporter -
  ACFELM_RS04825 (ACFELM_04825) - 1018388..1018660 (-) 273 WP_003094362.1 HPr family phosphocarrier protein -
  ACFELM_RS04830 (ACFELM_04830) rapZ 1018690..1019550 (-) 861 WP_003094361.1 RNase adapter RapZ -
  ACFELM_RS04835 (ACFELM_04835) ptsN 1019552..1020016 (-) 465 WP_003098853.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACFELM_RS04840 (ACFELM_04840) hpf 1020030..1020338 (-) 309 WP_003094359.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ACFELM_RS04845 (ACFELM_04845) rpoN/rpoN1 1020416..1021909 (-) 1494 WP_003094357.1 RNA polymerase factor sigma-54 Machinery gene
  ACFELM_RS04850 (ACFELM_04850) lptB 1022135..1022860 (-) 726 WP_003094355.1 LPS export ABC transporter ATP-binding protein -
  ACFELM_RS04855 (ACFELM_04855) lptA 1022860..1023387 (-) 528 WP_003120916.1 lipopolysaccharide transport periplasmic protein LptA -
  ACFELM_RS04860 (ACFELM_04860) lptC 1023374..1023946 (-) 573 WP_003094353.1 LPS export ABC transporter periplasmic protein LptC -
  ACFELM_RS04865 (ACFELM_04865) - 1023955..1024494 (-) 540 WP_003098849.1 HAD family hydrolase -
  ACFELM_RS04870 (ACFELM_04870) kdsD 1024494..1025474 (-) 981 WP_003094347.1 arabinose-5-phosphate isomerase KdsD -
  ACFELM_RS04875 (ACFELM_04875) - 1025888..1026697 (+) 810 WP_003094343.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 56054.22 Da        Isoelectric Point: 4.4327

>NTDB_id=954910 ACFELM_RS04845 WP_003094357.1 1020416..1021909(-) (rpoN/rpoN1) [Pseudomonas aeruginosa strain 238636E_ID015]
MKPSLVLKMGQQLTMTPQLQQAIRLLQLSTLDLQQEIQEALESNPMLERQEDGDDFDNSDPLADGAEQAASAPQESPLQE
SATPSVESLDDDQWSERIPSELPVDTAWEDIYQTSASSLPSNDDDEWDFTARTSSGESLHSHLLWQVNLAPMSDTDRMIA
VTIIDSINNDGYLEESLEEILAAIDPELDVELDEVEVVLRRIQQLEPAGIGARNLRECLLLQLRQLPSTTPWLNEALRLV
SDYLDLLGGRDYSQLMRRMKLKEDELRQVIELIQCLHPRPGSQIESSEAEYIVPDVIVRKDNERWLVELNQEAMPRLRVN
ATYAGMVRRADSSADNTFMRNQLQEARWFIKSLQSRNETLMKVATQIVEHQRGFLDYGEEAMKPLVLHDIAEAVGMHEST
ISRVTTQKYMHTPRGIFELKYFFSSHVSTAEGGECSSTAIRAIIKKLVAAENAKKPLSDSKIAGLLEAQGIQVARRTVAK
YRESLGIAPSSERKRLV

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=954910 ACFELM_RS04845 WP_003094357.1 1020416..1021909(-) (rpoN/rpoN1) [Pseudomonas aeruginosa strain 238636E_ID015]
ATGAAACCATCGCTAGTCCTCAAGATGGGCCAGCAGCTGACGATGACGCCGCAGCTGCAACAGGCCATACGCCTCCTCCA
GCTATCCACCCTGGACCTCCAGCAGGAAATCCAGGAAGCGCTGGAATCCAATCCCATGCTCGAACGCCAGGAAGACGGCG
ACGATTTCGACAACAGCGACCCTCTCGCCGATGGCGCCGAACAGGCGGCCAGCGCGCCCCAGGAGAGCCCCCTCCAGGAA
AGCGCCACGCCCAGCGTCGAAAGCCTCGACGACGACCAGTGGAGCGAACGCATACCCAGCGAGTTGCCGGTGGATACCGC
CTGGGAAGACATCTACCAGACCAGCGCCAGCAGCCTGCCCAGCAACGACGATGACGAGTGGGACTTCACCGCGCGCACCA
GCAGCGGCGAGAGCCTGCACAGCCACCTGCTCTGGCAGGTCAACCTGGCACCGATGTCGGACACCGATCGGATGATCGCG
GTGACCATCATCGATAGCATCAACAACGATGGCTACCTCGAAGAGTCCCTCGAAGAAATCCTCGCCGCCATCGATCCGGA
ACTGGACGTCGAGCTCGACGAAGTGGAAGTCGTGCTGCGCCGCATCCAGCAACTGGAACCCGCCGGCATCGGCGCCCGCA
ACCTGCGCGAGTGCCTGCTGCTGCAACTGCGCCAGTTGCCGTCCACCACGCCCTGGCTGAACGAAGCGCTGCGCCTGGTA
TCCGACTACCTCGACCTGCTCGGCGGCCGCGACTACAGCCAATTGATGCGGCGCATGAAGCTCAAGGAAGACGAACTGCG
CCAGGTCATCGAGCTGATCCAGTGCCTGCATCCCCGGCCCGGTTCGCAGATCGAATCGAGCGAAGCCGAATACATCGTCC
CCGACGTCATCGTGCGCAAGGACAACGAGCGCTGGCTGGTCGAGCTGAACCAGGAGGCGATGCCACGCCTGCGCGTCAAC
GCCACCTACGCCGGCATGGTCCGCCGCGCCGACAGCAGCGCCGACAATACCTTCATGCGCAACCAGTTGCAGGAAGCCCG
CTGGTTCATCAAGAGCCTGCAGAGCCGCAACGAAACGCTGATGAAGGTCGCCACCCAGATCGTCGAGCACCAGCGCGGCT
TCCTCGACTACGGCGAAGAAGCCATGAAGCCGCTGGTCCTGCACGACATCGCCGAAGCGGTGGGCATGCACGAATCGACC
ATTTCGCGGGTCACCACGCAGAAATACATGCATACGCCGCGCGGCATTTTCGAGCTGAAATATTTCTTTTCAAGTCACGT
CAGTACCGCCGAAGGCGGGGAATGCTCGTCCACCGCGATCCGCGCGATCATCAAGAAACTGGTGGCCGCGGAAAATGCGA
AAAAGCCATTGAGCGACAGCAAGATCGCTGGTTTACTGGAGGCACAGGGCATTCAAGTGGCTCGCCGCACCGTCGCCAAA
TACCGCGAATCCCTGGGCATAGCCCCTTCGAGCGAGCGCAAGCGACTGGTGTGA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(337-495)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(129-323)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZGR9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

46.654

100

0.477