Detailed information NTG0954767

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: ACFELB_RS28175
• Genome accession: NZ_CP172850
• Coordinates: 6059532..6061751 (+)
• Length: 739 a.a.
• NCBI ID: WP_023085815.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain 238638E_ID017
• Function: DNA puliing through the inner membrane (predicted from homology) DNA binding and uptake

Genomic Context

Location: 6054532..6066751

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACFELB_RS28155 (ACFELB_28155)	-	6055507..6056775 (-)	1269	WP_003103278.1	malic enzyme	-
ACFELB_RS28160 (ACFELB_28160)	-	6056878..6058317 (-)	1440	WP_003095844.1	M48 family metalloprotease	-
ACFELB_RS28165 (ACFELB_28165)	-	6058314..6059081 (-)	768	WP_003103280.1	thermonuclease family protein	-
ACFELB_RS28170 (ACFELB_28170)	rpmE	6059141..6059356 (-)	216	WP_003095846.1	50S ribosomal protein L31	-
ACFELB_RS28175 (ACFELB_28175)	priA	6059532..6061751 (+)	2220	WP_023085815.1	primosomal protein N'	Machinery gene
ACFELB_RS28180 (ACFELB_28180)	argS	6062001..6063764 (+)	1764	WP_181855472.1	arginine--tRNA ligase	-
ACFELB_RS28185 (ACFELB_28185)	-	6063799..6064494 (+)	696	WP_003095850.1	SPOR domain-containing protein	-
ACFELB_RS28190 (ACFELB_28190)	hslV	6064613..6065146 (+)	534	WP_003095852.1	ATP-dependent protease subunit HslV	-
ACFELB_RS28195 (ACFELB_28195)	hslU	6065175..6066518 (+)	1344	WP_003095854.1	ATP-dependent protease ATPase subunit HslU	-

Sequence

Protein

• Length: 739 a.a.
• Molecular weight: 82123.62 Da
• Isoelectric Point: 8.9189

>NTDB_id=954767 ACFELB_RS28175 WP_023085815.1 6059532..6061751(+) (priA) [Pseudomonas aeruginosa strain 238638E_ID017]
MSDLILRLALPSPLRRLFDYRAPRGIPRSALQPGIRLLLPFGRRELVGVLIEVTDRSEVPEDKLKPALRVLDAKPPMPAH
LLELCRWTAQYYQHSLGDTLSWALPNLLRQGEPAEARQQRFWHATAQSSLDDPRLARAPRQRQALAILKQHPHGVSHELL
NQLQINKDSLDLLKEKGLVELEVRRHSTPPREGGWLAQAELPLNPEQRAAFEAVRASHGGFHCFLLAGVTGSGKTEVYLQ
LIRESLAAGRQALVLIPEINLGPQTLARFERRFNARIALLHSALTDRERLDAWLAARDGEADIVIGTRSALFTPLKRPGL
IIVDEEHDASYKQQDGLRYHARDLALVRARLENVPILLGSATPALESLHNAQAGRYGLLRLTQRAGGAHPPKFIRLDVKS
MPLDAGLSRPLQQAIGDTLAAGQQVLVFLNRRGFAPTLLCHDCGWISQCPRCDARMTVHQGSGELRCHHCDHRQRPPMNC
PQCGKLDLRPVGAGTERAEERLRILFPNHPVLRIDRDSTSRKHAMRDLFATINSGEPCILVGTQMLAKGHHFPRVTLVAI
LDADGGLFSADFRASERMAQQIVQVAGRAGRAEEPGRVLIQTHLADHPLLVQLTEDGYFAFAEQALSERRAAGLPPFAHL
ALLRAEAHKPGQAEAFLDSACSAAEQLLEQMGGPEVELLGPVPAPMERRAGKHRAQLLLQCMSRAPLHRLLTPWLQSLEQ
LPGGRQVRWSLDIDPIDLF

Nucleotide

• Length: 2220 bp

>NTDB_id=954767 ACFELB_RS28175 WP_023085815.1 6059532..6061751(+) (priA) [Pseudomonas aeruginosa strain 238638E_ID017]
GTGTCAGACCTGATCCTACGCCTAGCCCTGCCTTCGCCACTCCGCCGCCTGTTCGACTACCGCGCGCCCCGCGGCATTCC
GCGCAGCGCCCTACAGCCGGGGATTCGCCTGCTGCTGCCGTTCGGGCGCCGGGAGCTGGTTGGCGTTCTGATAGAGGTGA
CGGACCGTTCCGAAGTACCCGAAGACAAGCTCAAGCCTGCCCTCAGGGTACTCGACGCCAAGCCGCCGATGCCGGCGCAC
CTGCTCGAACTCTGTCGCTGGACCGCCCAGTACTACCAGCACAGCCTCGGCGACACCCTGTCCTGGGCGCTGCCCAACCT
GTTGCGCCAGGGCGAGCCGGCCGAGGCGCGCCAGCAGCGCTTCTGGCATGCCACCGCGCAATCCAGCCTCGACGATCCGC
GCCTGGCCCGGGCGCCGCGCCAGCGCCAGGCCCTGGCGATTCTCAAGCAGCATCCGCACGGGGTGTCCCATGAGTTGCTG
AACCAGTTGCAGATAAACAAGGACAGCCTCGACCTGCTCAAGGAAAAAGGCTTGGTCGAACTGGAGGTGCGGCGCCACAG
CACCCCGCCGCGCGAAGGCGGCTGGCTGGCCCAGGCGGAGTTGCCGCTGAACCCCGAGCAGCGCGCCGCCTTCGAGGCGG
TTCGCGCCAGCCACGGCGGTTTCCATTGCTTCCTGCTGGCCGGGGTCACCGGCAGCGGCAAGACCGAGGTCTACCTGCAA
CTGATCCGCGAAAGCCTGGCCGCAGGCCGCCAGGCCCTGGTGCTGATTCCCGAGATCAACCTGGGGCCGCAGACCCTGGC
GCGTTTCGAACGCCGCTTCAACGCGCGCATCGCCCTGCTGCATTCGGCCCTGACCGACCGCGAGCGCCTCGACGCCTGGC
TCGCCGCCCGGGATGGCGAGGCCGACATCGTCATCGGCACCCGTTCGGCACTGTTCACTCCATTGAAGCGGCCGGGACTG
ATCATCGTCGACGAGGAACACGACGCCTCCTATAAACAGCAGGACGGCCTGCGCTACCACGCCCGCGACCTGGCACTGGT
ACGCGCACGCCTGGAGAACGTGCCGATCCTGCTCGGTTCGGCCACGCCCGCCCTGGAAAGCCTGCACAACGCCCAGGCCG
GCCGCTACGGCCTGCTGCGCCTGACCCAGCGCGCCGGTGGCGCGCACCCGCCGAAGTTCATCCGCCTCGACGTGAAGAGC
ATGCCGCTCGACGCCGGCCTTTCCCGTCCGCTGCAACAGGCCATCGGCGATACCCTGGCGGCCGGCCAGCAGGTACTGGT
GTTCCTCAACCGCCGCGGCTTCGCTCCGACCCTGCTTTGCCACGACTGCGGCTGGATCAGCCAATGCCCGCGCTGCGACG
CGCGGATGACCGTGCACCAGGGCAGCGGCGAGTTGCGCTGCCACCACTGCGACCACCGCCAGCGGCCGCCGATGAACTGC
CCGCAATGCGGCAAGCTCGACCTGCGCCCGGTAGGCGCCGGCACCGAGCGCGCCGAGGAACGCCTGCGGATTCTCTTCCC
GAACCATCCGGTGCTGCGCATCGACCGCGACAGCACCTCGCGCAAGCACGCCATGCGCGACCTCTTCGCCACCATCAACA
GCGGTGAGCCATGCATCCTGGTCGGCACCCAGATGCTCGCCAAGGGGCACCACTTCCCACGGGTCACCCTGGTGGCGATC
CTCGATGCCGATGGCGGCCTGTTTTCCGCCGACTTCCGCGCCAGCGAACGCATGGCCCAGCAGATCGTCCAGGTCGCCGG
CCGCGCAGGACGCGCCGAGGAGCCCGGGCGGGTACTGATCCAGACCCACCTGGCCGATCACCCGCTGCTGGTCCAGCTCA
CCGAGGACGGCTACTTCGCCTTTGCCGAACAGGCCCTCAGCGAACGCCGCGCCGCCGGCCTGCCGCCCTTCGCCCACCTC
GCCCTGCTGCGCGCCGAAGCGCACAAGCCGGGCCAGGCGGAAGCCTTCCTCGACAGCGCCTGCAGTGCCGCCGAGCAATT
GCTCGAGCAGATGGGCGGCCCGGAGGTGGAACTGCTCGGGCCGGTGCCCGCGCCCATGGAGCGGCGTGCCGGCAAGCACC
GCGCGCAACTGCTGCTGCAGTGCATGAGCCGGGCGCCGCTGCATCGCCTGCTGACGCCCTGGCTGCAGAGCCTCGAACAG
TTGCCCGGCGGGCGCCAGGTGCGCTGGTCGCTCGATATCGATCCGATCGACCTGTTCTGA

Domains

Predicted by InterProScan.

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (449-475)

• DEAD/DEAH box helicase (DEAD) (207-367)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (9-105)

• Primosomal protein N C-terminal domain (PriA_C) (636-735)

• Helicase conserved C-terminal domain (Helicase_C) (522-592)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Acinetobacter baumannii D1279779	47.613	99.188	0.472