Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACFELC_RS24155 Genome accession   NZ_CP172849
Coordinates   5164681..5166174 (+) Length   497 a.a.
NCBI ID   WP_033970527.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 238641E_ID020     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5159681..5171174
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFELC_RS24125 (ACFELC_24125) - 5159893..5160702 (-) 810 WP_003094343.1 ABC transporter ATP-binding protein -
  ACFELC_RS24130 (ACFELC_24130) kdsD 5161116..5162096 (+) 981 WP_170868904.1 arabinose-5-phosphate isomerase KdsD -
  ACFELC_RS24135 (ACFELC_24135) - 5162096..5162635 (+) 540 WP_003094350.1 HAD family hydrolase -
  ACFELC_RS24140 (ACFELC_24140) lptC 5162644..5163216 (+) 573 WP_003094353.1 LPS export ABC transporter periplasmic protein LptC -
  ACFELC_RS24145 (ACFELC_24145) lptA 5163203..5163730 (+) 528 WP_003120916.1 lipopolysaccharide transport periplasmic protein LptA -
  ACFELC_RS24150 (ACFELC_24150) lptB 5163730..5164455 (+) 726 WP_003094355.1 LPS export ABC transporter ATP-binding protein -
  ACFELC_RS24155 (ACFELC_24155) rpoN/rpoN1 5164681..5166174 (+) 1494 WP_033970527.1 RNA polymerase factor sigma-54 Machinery gene
  ACFELC_RS24160 (ACFELC_24160) hpf 5166252..5166560 (+) 309 WP_003094359.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ACFELC_RS24165 (ACFELC_24165) ptsN 5166574..5167038 (+) 465 WP_003098853.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACFELC_RS24170 (ACFELC_24170) rapZ 5167040..5167900 (+) 861 WP_003094361.1 RNase adapter RapZ -
  ACFELC_RS24175 (ACFELC_24175) - 5167930..5168202 (+) 273 WP_033970526.1 HPr family phosphocarrier protein -
  ACFELC_RS24180 (ACFELC_24180) - 5168299..5169231 (-) 933 WP_079387315.1 ZIP family metal transporter -
  ACFELC_RS24185 (ACFELC_24185) - 5169250..5169861 (-) 612 WP_083557176.1 superoxide dismutase -
  ACFELC_RS24190 (ACFELC_24190) - 5169874..5170323 (-) 450 WP_003094369.1 hypothetical protein -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 56068.24 Da        Isoelectric Point: 4.4351

>NTDB_id=954644 ACFELC_RS24155 WP_033970527.1 5164681..5166174(+) (rpoN/rpoN1) [Pseudomonas aeruginosa strain 238641E_ID020]
MKPSLVLKMGQQLTMTPQLQQAIRLLQLSTLDLQQEIQEALESNPMLERQEDGDDFDNSDPLADGAEQAASAPQESPLQE
SATPSVESLDDDQWSERIPSELPVDTAWEDIYQTSASSLPSNDDDEWDFTARTSSGESLHSHLLWQVNLAPMSDTDRMIA
VTIIDSINNDGYLEESLEEILAAIDPELDVELDEVEVVLRRIQQLEPAGIGARNLRECLLLQLRQLPSTTPWLNEALRLV
SDYLDLLGGRDYSQLMRRMKLKEEELRQVIELIQCLHPRPGSQIESSEAEYIVPDVIVRKDNERWLVELNQEAMPRLRVN
ATYAGMVRRADSSADNTFMRNQLQEARWFIKSLQSRNETLMKVATQIVEHQRGFLDYGEEAMKPLVLHDIAEAVGMHEST
ISRVTTQKYMHTPRGIFELKYFFSSHVSTAEGGECSSTAIRAIIKKLVAAENAKKPLSDSKIAGLLEAQGIQVARRTVAK
YRESLGIAPSSERKRLV

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=954644 ACFELC_RS24155 WP_033970527.1 5164681..5166174(+) (rpoN/rpoN1) [Pseudomonas aeruginosa strain 238641E_ID020]
ATGAAACCATCGCTAGTCCTCAAGATGGGCCAGCAGCTGACGATGACGCCGCAGCTGCAACAGGCCATACGCCTCCTCCA
GCTATCCACCCTGGACCTCCAGCAGGAAATCCAGGAAGCGCTGGAATCCAACCCCATGCTCGAACGCCAGGAAGACGGCG
ACGACTTCGACAACAGCGACCCCCTCGCCGATGGCGCCGAACAGGCGGCCAGCGCGCCCCAGGAGAGCCCCCTCCAGGAA
AGCGCCACGCCCAGCGTCGAAAGCCTCGACGACGACCAGTGGAGCGAACGCATTCCCAGCGAGTTGCCGGTGGATACCGC
CTGGGAAGACATCTACCAGACCAGCGCCAGCAGCCTGCCCAGCAACGACGATGACGAATGGGACTTCACCGCGCGCACCA
GCAGCGGCGAGAGCCTGCACAGCCACCTGCTCTGGCAGGTCAACCTGGCACCGATGTCGGACACCGACCGGATGATCGCG
GTGACCATCATCGATAGCATCAACAACGATGGCTATCTCGAAGAGTCCCTCGAAGAAATCCTCGCCGCCATCGATCCGGA
ACTGGACGTCGAGCTCGACGAAGTGGAAGTCGTGCTGCGCCGCATCCAGCAACTGGAACCCGCCGGCATCGGCGCTCGCA
ACCTGCGCGAGTGCCTGCTGCTGCAACTGCGCCAGTTGCCGTCCACCACGCCCTGGCTGAACGAAGCGCTGCGCCTGGTA
TCCGACTACCTCGACCTGCTCGGCGGCCGCGACTACAGCCAATTGATGCGGCGCATGAAGCTCAAGGAAGAGGAACTGCG
CCAGGTCATCGAGCTGATCCAGTGCCTGCATCCCCGGCCCGGTTCGCAGATCGAATCGAGCGAAGCCGAATACATCGTCC
CCGACGTCATCGTGCGCAAGGACAACGAGCGCTGGCTGGTCGAGCTGAACCAGGAGGCGATGCCGCGCTTGCGCGTCAAC
GCCACCTACGCCGGCATGGTCCGCCGCGCCGACAGCAGCGCCGACAATACCTTCATGCGCAACCAGTTGCAGGAAGCCCG
CTGGTTCATCAAGAGCCTGCAGAGCCGCAACGAAACGCTGATGAAGGTCGCCACCCAGATCGTCGAGCACCAGCGCGGTT
TCCTCGACTACGGCGAAGAAGCCATGAAGCCGCTGGTCCTGCACGACATCGCCGAAGCGGTGGGCATGCACGAATCGACC
ATTTCGCGGGTCACCACGCAGAAATACATGCATACGCCGCGCGGCATTTTCGAGCTGAAATATTTCTTTTCAAGTCACGT
CAGCACCGCCGAAGGCGGGGAATGCTCGTCCACCGCAATCCGCGCGATCATCAAGAAACTGGTGGCCGCGGAAAATGCGA
AAAAGCCATTGAGCGACAGCAAGATCGCTGGTTTACTGGAGGCACAGGGCATTCAAGTGGCTCGCCGCACCGTCGCCAAA
TACCGCGAATCCCTGGGCATAGCCCCTTCGAGCGAGCGCAAGCGACTGGTGTGA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(337-495)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(129-323)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

46.654

100

0.477