Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   WHP50_RS05525 Genome accession   NZ_CP148264
Coordinates   1070318..1071268 (+) Length   316 a.a.
NCBI ID   WP_002899461.1    Uniprot ID   A0A5Z1S6J2
Organism   Campylobacter jejuni strain Z1322HCJ0003     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1054793..1084257 1070318..1071268 within 0


Gene organization within MGE regions


Location: 1054793..1084257
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WHP50_RS05455 (WHP50_05455) pglI 1054793..1055722 (-) 930 WP_032583777.1 GalNAc(5)-diNAcBac-PP-undecaprenol beta-1,3-glucosyltransferase -
  WHP50_RS05460 (WHP50_05460) pglH 1055715..1056794 (-) 1080 WP_338910871.1 GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)- diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase -
  WHP50_RS05465 (WHP50_05465) pglK 1056791..1058485 (-) 1695 WP_002899514.1 ABC-type lipopolysaccharide transporter PglK -
  WHP50_RS05470 (WHP50_05470) galE 1058479..1059465 (-) 987 WP_002874249.1 UDP-glucose 4-epimerase GalE -
  WHP50_RS05475 (WHP50_05475) - 1059524..1060318 (-) 795 WP_038813904.1 3'-5' exonuclease -
  WHP50_RS05480 (WHP50_05480) waaC 1060385..1061413 (+) 1029 WP_002899517.1 lipopolysaccharide heptosyltransferase I -
  WHP50_RS05485 (WHP50_05485) htrB 1061403..1062290 (+) 888 WP_002899518.1 lipid A biosynthesis lauroyl acyltransferase HtrB -
  WHP50_RS05490 (WHP50_05490) - 1062287..1063054 (+) 768 Protein_1060 glycosyltransferase family 2 protein -
  WHP50_RS05495 (WHP50_05495) - 1063047..1064108 (+) 1062 WP_002899522.1 glycosyltransferase family 4 protein -
  WHP50_RS05500 (WHP50_05500) - 1064100..1064966 (-) 867 WP_002899525.1 glycosyltransferase family 2 protein -
  WHP50_RS05505 (WHP50_05505) - 1064959..1066173 (-) 1215 WP_002899528.1 glycosyltransferase family 2 protein -
  WHP50_RS05510 (WHP50_05510) - 1066166..1068097 (-) 1932 WP_052785346.1 hypothetical protein -
  WHP50_RS05515 (WHP50_05515) - 1068173..1069447 (+) 1275 WP_052785345.1 DUF2972 domain-containing protein -
  WHP50_RS05520 (WHP50_05520) - 1069411..1070259 (-) 849 WP_002899460.1 glycosyltransferase family 2 protein -
  WHP50_RS05525 (WHP50_05525) waaF 1070318..1071268 (+) 951 WP_002899461.1 lipopolysaccharide heptosyltransferase II Regulator
  WHP50_RS05530 (WHP50_05530) - 1071254..1072018 (-) 765 WP_002899462.1 glycosyltransferase family 25 protein -
  WHP50_RS05535 (WHP50_05535) gmhA 1072018..1072578 (-) 561 WP_002881439.1 D-sedoheptulose 7-phosphate isomerase -
  WHP50_RS05540 (WHP50_05540) rfaE1 1072575..1073960 (-) 1386 WP_052785344.1 D-glycero-beta-D-manno-heptose-7-phosphate kinase -
  WHP50_RS05545 (WHP50_05545) rfaD 1073953..1074906 (-) 954 WP_038813706.1 ADP-glyceromanno-heptose 6-epimerase -
  WHP50_RS05550 (WHP50_05550) gmhB 1074907..1075467 (-) 561 WP_052794772.1 D-glycero-alpha-D-manno-heptose-1,7-bisphosphate 7-phosphatase -
  WHP50_RS05555 (WHP50_05555) - 1075549..1075851 (+) 303 WP_002852762.1 cytochrome c -
  WHP50_RS05560 (WHP50_05560) ccoS 1075874..1076080 (-) 207 WP_002852798.1 cbb3-type cytochrome oxidase assembly protein CcoS -
  WHP50_RS05565 (WHP50_05565) - 1076077..1078434 (-) 2358 WP_074484153.1 heavy metal translocating P-type ATPase -
  WHP50_RS05570 (WHP50_05570) rho 1078543..1079841 (+) 1299 WP_002853705.1 transcription termination factor Rho -
  WHP50_RS05575 (WHP50_05575) dnaX 1079845..1081374 (+) 1530 WP_038813895.1 DNA polymerase III subunit gamma/tau -
  WHP50_RS05580 (WHP50_05580) - 1081371..1081862 (-) 492 WP_002891839.1 DUF2165 family protein -
  WHP50_RS05585 (WHP50_05585) copA 1081964..1084063 (-) 2100 WP_002892797.1 copper-translocating P-type ATPase CopA -
  WHP50_RS05590 (WHP50_05590) - 1084063..1084257 (-) 195 WP_002852760.1 cation transporter -

Sequence

Protein


Download         Length: 316 a.a.        Molecular weight: 36265.84 Da        Isoelectric Point: 10.2322

>NTDB_id=953623 WHP50_RS05525 WP_002899461.1 1070318..1071268(+) (waaF) [Campylobacter jejuni strain Z1322HCJ0003]
MKIFIHLPTWLGDAVMASPALYTIKEHFKDAQFILYGSFVSTALFKEFPNSKIIIENKQSRYKQALSLRKELGKIDLSFA
FRSAFSSKIILHILKTKQRYFFDKNKHKEEHQVLKYLYFIENSLGIKAHFKDLKLPFKLKFQNPLILKNGKKILGLNPGA
SFGSAKRWDASYFAKVALNFSQSHEILIFGAGKAEQELCNEIYQILKEQNIKVKNLCNKTTIKTLCQNIAFCDLFITNDS
GPMHLSAVYKVKTVAIFGPTKFTQTSPWQNENAKLVHLDLACMPCMQKTCPLKHHKCMKDLKPEVILKAIQNLINI

Nucleotide


Download         Length: 951 bp        

>NTDB_id=953623 WHP50_RS05525 WP_002899461.1 1070318..1071268(+) (waaF) [Campylobacter jejuni strain Z1322HCJ0003]
ATGAAAATTTTTATACATCTTCCCACTTGGCTAGGCGATGCAGTAATGGCTTCACCTGCTTTATACACTATAAAAGAACA
TTTTAAAGATGCTCAGTTTATCCTTTATGGTTCTTTTGTTTCTACAGCACTTTTTAAAGAATTTCCTAATTCTAAAATCA
TCATAGAAAATAAACAATCACGCTACAAACAAGCCCTATCTTTACGCAAAGAACTTGGTAAAATCGATCTTAGTTTTGCT
TTTAGATCTGCGTTTTCTTCTAAGATTATCTTGCATATTCTTAAAACAAAACAAAGATATTTTTTTGACAAAAACAAGCA
CAAAGAAGAACATCAAGTTTTAAAATACCTTTATTTTATAGAAAACTCACTTGGCATAAAAGCTCATTTTAAGGACTTAA
AGCTTCCCTTTAAGCTAAAATTTCAAAACCCTCTTATCTTAAAAAATGGTAAAAAAATTCTAGGACTCAACCCTGGTGCA
AGCTTTGGAAGTGCAAAAAGATGGGATGCGAGTTATTTTGCTAAAGTGGCTTTAAATTTCAGCCAAAGTCATGAGATTTT
AATCTTTGGTGCAGGAAAAGCCGAACAAGAACTTTGTAATGAAATTTATCAAATTTTAAAAGAACAAAACATAAAAGTAA
AAAATCTTTGCAATAAAACCACCATCAAAACCCTTTGTCAAAATATCGCTTTTTGCGATCTTTTCATCACAAATGATAGT
GGCCCTATGCACCTAAGTGCGGTTTATAAGGTAAAAACCGTGGCTATTTTTGGTCCTACGAAATTTACTCAAACTTCACC
TTGGCAAAACGAAAATGCAAAATTAGTACATTTAGATCTAGCTTGTATGCCTTGTATGCAAAAAACCTGCCCTTTAAAAC
ACCACAAATGCATGAAAGATCTAAAGCCTGAAGTGATTTTAAAGGCAATTCAAAATTTAATTAATATCTAA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(94-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5Z1S6J2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

95.541

99.367

0.949