Detailed information    

experimental Experimentally validated

Overview

Name   waaF   Type   Regulator
Locus tag   Cj1148 Genome accession   NC_002163
Coordinates   1080908..1081867 (+) Length   319 a.a.
NCBI ID   YP_002344539.1    Uniprot ID   Q0P9A8
Organism   Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819     
Function   repress natural transformation   
Competence regulation

Function

Loss of CPS and truncation of LOS increased the transformation frequency by 4- and 25-fold, respectively, and mutation of both CPS and LOS resulted in a 97-fold increase in the transformation frequency. Consistent with the increased transformation frequencies, the CPS and LOS mutants showed enhanced rates of DNA uptake.


Genomic Context

Location: 1075908..1086867
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Cj1142 neuC1 1076478..1077593 (+) 1116 YP_002344535.1 UDP-N-acetylglucosamine 2-epimerase -
  Cj1143 neuA1 1077590..1079200 (+) 1611 YP_002344536.1 bifunctional beta-1,4-N-acetylgalactosaminyltransferase/CMP-Neu5Ac synthase -
  Cj1146c waaV 1080025..1080849 (-) 825 YP_002344538.1 glucosyltransferase -
  Cj1148 waaF 1080908..1081867 (+) 960 YP_002344539.1 heptosyltransferase II Regulator
  Cj1149c gmhA 1081848..1082408 (-) 561 YP_002344540.1 phosphoheptose isomerase -
  Cj1150c hldE 1082405..1083790 (-) 1386 YP_002344541.1 bifunctional D-beta-D-heptose 7-phosphate kinase/D-beta-D-heptose 1-phosphate adenylyltransferase -
  Cj1151c hldD 1083783..1084736 (-) 954 YP_002344542.1 ADP-glyceromanno-heptose 6-epimerase -
  Cj1152c gmhB 1084737..1085297 (-) 561 YP_002344543.1 D-glycero-alpha-D-manno-heptose-1,7-bisphosphate 7-phosphatase -
  Cj1153 - 1085379..1085681 (+) 303 YP_002344544.1 cytochrome C -
  Cj1154c - 1085704..1085910 (-) 207 YP_002344545.1 cbb3-type cytochrome oxidase maturation protein -

Sequence

Protein


Download         Length: 319 a.a.        Molecular weight: 36734.30 Da        Isoelectric Point: 10.2412

>NTDB_id=1241 Cj1148 YP_002344539.1 1080908..1081867(+) (waaF) [Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819]
MKIFIHLPTWLGDTVMASPALYTIKEHFKDAQFILYGSFVSTALFKEFPNSKIIIENKLSRYKQALSLRKELGKIDLSFA
FRSAFSSKIILHILKTKQRYFFDKNKYKEEHQVLKYLYFIENSLSIKAHFKDLKLPFKLKFQNPLILRNGKKILGLNPGA
SFGSAKRWDASYFAKVALNFSQSHDILIFGAGKAEQELCNEIYQILKEQNIKVKNLCNKTTIKTLCQNIAFCDLFITNDS
GPMHLSAVYKVKTVAIFGPTKFTQTSPWQNENAKLVHLDLACMPCMQKTCPLKHHKCMKDLKPEKVIEQAKNLLKNSHL

Nucleotide


Download         Length: 960 bp        

>NTDB_id=1241 Cj1148 YP_002344539.1 1080908..1081867(+) (waaF) [Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819]
ATGAAAATTTTTATACATCTTCCCACCTGGTTAGGCGATACGGTAATGGCTTCACCTGCTTTATACACTATAAAAGAACA
TTTTAAAGATGCTCAGTTTATCCTTTATGGTTCTTTTGTTTCTACAGCACTTTTTAAAGAATTTCCTAATTCTAAAATCA
TCATAGAAAATAAACTATCCCGTTATAAACAAGCTCTATCTTTACGCAAAGAACTTGGTAAAATCGATCTTAGCTTTGCT
TTTAGATCTGCGTTCTCTTCTAAGATTATCTTGCATATCCTTAAAACAAAACAAAGATATTTTTTTGACAAAAACAAGTA
CAAAGAAGAACATCAAGTTTTAAAATACCTTTATTTTATAGAAAATTCACTTAGTATAAAAGCTCATTTTAAGGACTTAA
AGCTTCCCTTTAAGCTAAAATTTCAAAACCCTCTTATCTTAAGAAATGGTAAAAAAATTCTAGGACTCAACCCTGGTGCA
AGCTTTGGAAGTGCAAAAAGATGGGATGCGAGTTATTTTGCTAAAGTGGCTTTAAATTTCAGCCAAAGTCATGATATTTT
AATCTTTGGTGCAGGAAAAGCCGAACAAGAACTTTGTAATGAAATTTATCAAATTTTAAAAGAACAAAACATAAAAGTAA
AAAATCTTTGCAATAAAACCACCATCAAAACCCTTTGTCAAAATATCGCTTTTTGCGATCTTTTCATCACAAATGACAGT
GGACCTATGCACCTAAGTGCGGTTTATAAGGTAAAAACCGTAGCTATTTTTGGCCCTACGAAATTTACTCAAACTTCACC
TTGGCAAAATGAAAATGCAAAATTAGTGCATTTAGATCTAGCTTGTATGCCTTGTATGCAAAAAACCTGCCCTTTAAAAC
ACCACAAATGCATGAAAGATCTAAAGCCTGAAAAAGTGATAGAACAGGCTAAAAATTTACTTAAAAACTCTCATCTATGA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(94-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q0P9A8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Byeonghwa Jeon et al. (2009) Roles of lipooligosaccharide and capsular polysaccharide in antimicrobial resistance and natural transformation of Campylobacter jejuni. The Journal of Antimicrobial Chemotherapy 63(3):462-8. [PMID: 19147521]