Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACG4I1_RS08350 Genome accession   NZ_CP172323
Coordinates   1821757..1823220 (-) Length   487 a.a.
NCBI ID   WP_000804830.1    Uniprot ID   Q9KP49
Organism   Vibrio cholerae strain M815     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1816757..1828220
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACG4I1_RS08320 pmbA 1816899..1818242 (+) 1344 WP_000382645.1 metalloprotease PmbA -
  ACG4I1_RS08325 mgtE 1818314..1819675 (-) 1362 WP_000840452.1 magnesium transporter -
  ACG4I1_RS08330 - 1819836..1820114 (-) 279 WP_001135899.1 HPr family phosphocarrier protein -
  ACG4I1_RS08335 rapZ 1820123..1820983 (-) 861 WP_001237199.1 RNase adapter RapZ -
  ACG4I1_RS08340 ptsN 1820996..1821442 (-) 447 WP_001177163.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACG4I1_RS08345 hpf 1821445..1821732 (-) 288 WP_001166643.1 ribosome hibernation promoting factor -
  ACG4I1_RS08350 rpoN/rpoN1 1821757..1823220 (-) 1464 WP_000804830.1 RNA polymerase factor sigma-54 Machinery gene
  ACG4I1_RS08355 lptB 1823276..1824001 (-) 726 WP_000996178.1 LPS export ABC transporter ATP-binding protein -
  ACG4I1_RS08360 lptA 1824004..1824498 (-) 495 WP_000830444.1 lipopolysaccharide transport periplasmic protein LptA -
  ACG4I1_RS08365 lptC 1824479..1825042 (-) 564 WP_000059348.1 LPS export ABC transporter periplasmic protein LptC -
  ACG4I1_RS08370 kdsC 1825039..1825596 (-) 558 WP_000099768.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ACG4I1_RS08375 kdsD 1825596..1826570 (-) 975 WP_001120614.1 arabinose-5-phosphate isomerase KdsD -
  ACG4I1_RS08380 - 1826626..1827588 (-) 963 WP_000904330.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 487 a.a.        Molecular weight: 55438.47 Da        Isoelectric Point: 4.2824

>NTDB_id=951652 ACG4I1_RS08350 WP_000804830.1 1821757..1823220(-) (rpoN/rpoN1) [Vibrio cholerae strain M815]
MKPSLQLKLGQQLAMTPQLQQAIRLLQLSTLDLQQEIQEALESNPLLEVEENQDEAPSLDSVRMTEESPREPEELYEPEP
QDSSDLIEKSEISAELEMDTTWDEVYSANTGSTGLALDDDAPIYQGETTQTLQDYLHWQLDLTPFSDVDRTIAVALIDAI
DDYGYLTVSLEEIQESLRSDDIELDEIEAVRKRIQQFDPFGVASLNLQDCLLLQLTTYPCDTPWLEEARLLLSQYIDDLG
NRDYKTILKETKLKEEDLREILQLIQQLDPRPGSRIAQDHAEYVIPDVSVYKEQGRWLVTINPDSVPKLKINQQYADLMR
GNNAESNYIRTNLQEAKWLIKSLESRNETLLKVAKCIVEHQHDFFEYGEEAMKPMVLNDVAMAVEMHESTISRVTTQKYM
HTPRGIFELKYFFSSHVSTDNGGECSSTAIRALIKKLVAAENPAKPLSDSKIATLLADQGIQVARRTIAKYRESLGIAPS
SQRKRLL

Nucleotide


Download         Length: 1464 bp        

>NTDB_id=951652 ACG4I1_RS08350 WP_000804830.1 1821757..1823220(-) (rpoN/rpoN1) [Vibrio cholerae strain M815]
ATGAAACCGTCATTACAGCTCAAGCTAGGTCAACAGTTAGCCATGACTCCGCAGCTCCAGCAGGCGATTCGTCTGTTGCA
GCTTTCGACGCTGGATCTGCAGCAAGAAATTCAGGAAGCATTGGAATCTAACCCCTTGTTAGAGGTGGAAGAGAACCAAG
ACGAAGCGCCATCGCTTGATAGCGTACGTATGACGGAAGAAAGCCCGCGCGAGCCAGAAGAACTCTACGAACCTGAACCG
CAGGATAGCTCGGATCTGATTGAAAAATCGGAAATCAGCGCTGAATTAGAGATGGATACCACTTGGGATGAAGTCTACAG
CGCCAATACTGGCAGTACAGGACTGGCACTCGATGATGATGCCCCTATCTACCAAGGCGAAACCACCCAAACCCTGCAAG
ATTATCTTCACTGGCAACTCGACTTAACTCCCTTTAGCGATGTGGATCGCACTATAGCCGTCGCACTTATCGATGCTATC
GACGATTACGGTTATTTAACCGTCTCACTAGAGGAGATCCAAGAGAGCCTGCGCAGTGATGACATTGAGTTGGATGAGAT
TGAAGCGGTACGTAAACGCATTCAGCAGTTTGACCCCTTTGGTGTGGCATCGCTTAATCTGCAAGACTGCTTGTTGCTGC
AACTCACCACCTATCCCTGTGATACTCCTTGGCTGGAAGAAGCGCGTTTACTGCTCTCACAGTACATCGATGATTTAGGG
AATCGCGATTACAAAACCATTCTGAAAGAGACTAAGCTCAAAGAAGAGGACTTGCGCGAGATCCTGCAACTCATCCAACA
GCTCGATCCTCGCCCCGGCAGTCGAATTGCCCAAGATCACGCTGAATACGTCATACCTGACGTGTCAGTCTATAAAGAAC
AAGGTCGATGGCTGGTCACCATCAACCCAGATAGTGTGCCTAAACTGAAGATCAATCAGCAGTATGCCGACCTGATGCGC
GGTAATAATGCGGAGAGCAACTACATCCGTACCAATTTGCAAGAGGCAAAATGGCTGATCAAAAGTTTAGAAAGTAGAAA
CGAGACACTGCTCAAAGTTGCCAAGTGCATAGTTGAACACCAACACGATTTCTTCGAGTATGGAGAAGAGGCGATGAAGC
CGATGGTGCTCAACGATGTGGCGATGGCGGTGGAAATGCATGAATCGACAATTTCGCGTGTCACCACGCAGAAATACATG
CATACCCCGCGCGGCATTTTTGAGTTGAAGTACTTTTTCTCCAGCCACGTCAGTACTGACAATGGCGGAGAGTGCTCGTC
CACTGCGATCAGGGCGCTGATCAAAAAACTGGTGGCGGCAGAGAATCCTGCCAAACCACTCAGTGACAGCAAGATCGCAA
CCCTACTCGCTGACCAAGGCATCCAAGTCGCGCGGCGAACCATCGCCAAATACCGTGAATCACTTGGCATCGCCCCATCA
AGTCAGCGCAAACGCCTGCTATAG

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-49)

Sigma-54, DNA binding domain (Sigma54_DBD)

(327-485)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(128-315)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9KP49

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.533

100

0.46