Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   WHO18_RS13435 Genome accession   NZ_CP148128
Coordinates   2558593..2559663 (-) Length   356 a.a.
NCBI ID   WP_004399124.1    Uniprot ID   A0A809FNW6
Organism   Bacillus subtilis isolate FELIX_MS620     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2553593..2564663
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WHO18_RS13390 tapA 2554072..2554833 (-) 762 WP_004399106.1 amyloid fiber anchoring/assembly protein TapA -
  WHO18_RS13395 yqzG 2555105..2555431 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  WHO18_RS13400 spoIIT 2555473..2555652 (-) 180 WP_003230176.1 YqzE family protein -
  WHO18_RS13405 comGG 2555723..2556097 (-) 375 WP_003230170.1 ComG operon protein ComGG Machinery gene
  WHO18_RS13410 comGF 2556098..2556481 (-) 384 WP_003230168.1 ComG operon protein ComGF Machinery gene
  WHO18_RS13415 comGE 2556507..2556854 (-) 348 WP_003230165.1 ComG operon protein 5 Machinery gene
  WHO18_RS13420 comGD 2556838..2557269 (-) 432 WP_004398628.1 comG operon protein ComGD Machinery gene
  WHO18_RS13425 comGC 2557259..2557555 (-) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  WHO18_RS13430 comGB 2557569..2558606 (-) 1038 WP_009967727.1 comG operon protein ComGB Machinery gene
  WHO18_RS13435 comGA 2558593..2559663 (-) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  WHO18_RS13440 corA 2560075..2561028 (-) 954 WP_004399136.1 magnesium transporter CorA -
  WHO18_RS13445 yqhB 2561171..2562499 (+) 1329 WP_009967730.1 hemolysin family protein -
  WHO18_RS13450 rsbRD 2562552..2563388 (-) 837 WP_004398754.1 RsbT co-antagonist protein RsbRD -
  WHO18_RS13460 mgsR 2563612..2563992 (-) 381 WP_003230147.1 transcriptional regulator MgsR -
  WHO18_RS13465 yqgY 2564224..2564469 (+) 246 WP_003226300.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40458.64 Da        Isoelectric Point: 9.0175

>NTDB_id=951454 WHO18_RS13435 WP_004399124.1 2558593..2559663(-) (comGA) [Bacillus subtilis isolate FELIX_MS620]
MDSIEKVSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=951454 WHO18_RS13435 WP_004399124.1 2558593..2559663(-) (comGA) [Bacillus subtilis isolate FELIX_MS620]
TTGGATTCAATAGAAAAGGTAAGCAAAAACTTGATTGAAGAGGCATATCTAACAAAGGCTTCTGATATTCACATTGTGCC
GAGGGAGCGGGACGCTATCATTCATTTTCGGGTCGATCATGCCTTGCTGAAAAAAAGGGACATGAAAAAAGAAGAGTGCG
TAAGACTGATTTCACATTTTAAATTTCTTTCAGCAATGGATATAGGTGAAAGGCGAAAGCCGCAAAACGGTTCGCTTACG
TTAAAGTTGAAAGAGGGAAATGTTCATTTAAGAATGTCAACGCTGCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCCCAATACAATATCCCTTCGATTGATAAATTGTCGCTATTTCCGAAGACAGGAGCCACATTACTCTCGTTTTTAA
AACATTCCCATGGCATGCTCATTTTTACCGGGCCGACTGGTTCAGGGAAGACTACCACATTATACTCTCTCGTTCAATAT
GCAAAAAAACACTTTAATCGAAATATTGTCACATTAGAGGACCCTGTTGAAACAAGGGACGAAGATGTTCTTCAGGTTCA
GGTGAATGAAAAAGCCGGTGTAACTTATTCCGCAGGTCTGAAAGCAATTTTGCGCCATGACCCCGATATGATTATTTTAG
GTGAGATCAGAGACGCGGAAACAGCTGAAATTGCGGTGCGGGCAGCGATGACGGGACATCTGGTACTAACGAGCCTTCAT
ACGAGAGACGCAAAGGGCGCAATTTACAGACTGCTTGAATTCGGTATCAATATGAATGAAATCGAACAGACTGTCATTGC
AATAGCGGCTCAGCGCTTGGTTGATTTGGCTTGCCCGTTTTGTGAAAACGGATGTTCATCAGTGTATTGCCGACAGTCAC
GAAATACTAGGAGAGCTAGCGTTTATGAGCTTCTATACGGGAAAAATCTTCAGCAATGTATCCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTTCGTCAAATTATCAGAAAAGGAATTGCGCTCGGCTATTTAACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A809FNW6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

100

100

1