Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   WHO22_RS01985 Genome accession   NZ_CP148122
Coordinates   371752..372201 (-) Length   149 a.a.
NCBI ID   WP_003246436.1    Uniprot ID   A0AAE2SFQ0
Organism   Bacillus subtilis isolate FELIX_MS513     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 366752..377201
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WHO22_RS01950 yckA 366903..367583 (-) 681 WP_003234624.1 amino acid ABC transporter permease -
  WHO22_RS01955 yckB 367593..368456 (-) 864 WP_009966510.1 transporter substrate-binding domain-containing protein -
  WHO22_RS01960 - 368618..368815 (+) 198 WP_119122841.1 hypothetical protein -
  WHO22_RS01965 yckC 368834..369289 (+) 456 WP_003246356.1 RDD family protein -
  WHO22_RS01970 yckD 369371..369703 (+) 333 WP_003246247.1 YckD family protein -
  WHO22_RS01975 bglC 369857..371290 (+) 1434 WP_003246242.1 6-phospho-beta-glucosidase -
  WHO22_RS01980 nin/comJ 371327..371725 (-) 399 WP_003246342.1 DNA-entry nuclease inhibitor Regulator
  WHO22_RS01985 nucA/comI 371752..372201 (-) 450 WP_003246436.1 DNA-entry nuclease Machinery gene
  WHO22_RS01990 tlpC 372369..374090 (-) 1722 WP_003246498.1 methyl-accepting chemotaxis protein TlpC -
  WHO22_RS01995 hxlB 374201..374758 (-) 558 WP_003246343.1 6-phospho-3-hexuloisomerase -
  WHO22_RS02000 hxlA 374764..375396 (-) 633 WP_003246452.1 3-hexulose-6-phosphate synthase -
  WHO22_RS02005 hxlR 375629..375991 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16486.48 Da        Isoelectric Point: 4.6274

>NTDB_id=951190 WHO22_RS01985 WP_003246436.1 371752..372201(-) (nucA/comI) [Bacillus subtilis isolate FELIX_MS513]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETMAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHRLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=951190 WHO22_RS01985 WP_003246436.1 371752..372201(-) (nucA/comI) [Bacillus subtilis isolate FELIX_MS513]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATGGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAAGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
GGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(53-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

100

100

1