Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA/sms   Type   Machinery gene
Locus tag   ACHP5I_RS24715 Genome accession   NZ_CP172185
Coordinates   4948968..4950350 (-) Length   460 a.a.
NCBI ID   WP_001029698.1    Uniprot ID   A0A2T3RLH6
Organism   Escherichia coli strain GF60     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4943968..4955350
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHP5I_RS24670 (ACHP5I_24670) - 4944062..4944858 (+) 797 Protein_4852 SDR family NAD(P)-dependent oxidoreductase -
  ACHP5I_RS24675 (ACHP5I_24675) - 4944884..4945300 (+) 417 WP_001140838.1 SRPBCC family protein -
  ACHP5I_RS24680 (ACHP5I_24680) - 4945458..4945670 (+) 213 WP_000848792.1 DUF1471 domain-containing protein -
  ACHP5I_RS24685 (ACHP5I_24685) - 4945743..4945859 (+) 117 Protein_4855 hypothetical protein -
  ACHP5I_RS24690 (ACHP5I_24690) - 4945819..4945947 (+) 129 WP_000883916.1 hypothetical protein -
  ACHP5I_RS24695 (ACHP5I_24695) - 4946098..4946469 (-) 372 WP_001036747.1 winged helix-turn-helix domain-containing protein -
  ACHP5I_RS24700 (ACHP5I_24700) - 4946495..4946914 (-) 420 WP_249546546.1 sulfite exporter TauE/SafE family protein -
  ACHP5I_RS24705 (ACHP5I_24705) - 4946915..4947301 (-) 387 WP_249546547.1 TSUP family transporter -
  ACHP5I_RS24710 (ACHP5I_24710) nadR 4947715..4948947 (-) 1233 WP_000093813.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  ACHP5I_RS24715 (ACHP5I_24715) radA/sms 4948968..4950350 (-) 1383 WP_001029698.1 DNA repair protein RadA Machinery gene
  ACHP5I_RS24720 (ACHP5I_24720) serB 4950399..4951367 (-) 969 WP_001132956.1 phosphoserine phosphatase -
  ACHP5I_RS24725 (ACHP5I_24725) ytjB 4951473..4952117 (+) 645 WP_000124615.1 YtjB family periplasmic protein -
  ACHP5I_RS24730 (ACHP5I_24730) lplA 4952145..4953161 (+) 1017 WP_062875617.1 lipoate--protein ligase LplA -
  ACHP5I_RS24735 (ACHP5I_24735) - 4953193..4953456 (+) 264 WP_001589645.1 helix-turn-helix transcriptional regulator -
  ACHP5I_RS24740 (ACHP5I_24740) deoD 4953617..4954336 (-) 720 WP_000224879.1 purine-nucleoside phosphorylase -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49442.02 Da        Isoelectric Point: 7.1936

>NTDB_id=950998 ACHP5I_RS24715 WP_001029698.1 4948968..4950350(-) (radA/sms) [Escherichia coli strain GF60]
MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPTVARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTG
FKEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDH
SMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQDLVVFGEV
GLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKLSDALSVFDDL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=950998 ACHP5I_RS24715 WP_001029698.1 4948968..4950350(-) (radA/sms) [Escherichia coli strain GF60]
GTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGCGC
CTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCAGCGTCGCCAACGGTGGCGCGTAACGAGCGTCTCAGTGGCT
ATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTTTCCGACATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGA
TTTAAAGAGTTCGACCGCGTATTAGGCGGCGGGGTGGTGCCGGGAAGCGCCATTCTGATTGGCGGTAACCCAGGCGCGGG
GAAATCCACGCTGCTGTTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGC
TGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGCTGTCTGAAACCAGCATCGAA
CAAATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGT
TCAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGG
CGATTGTCATGGTTGGGCACGTAACCAAAGATGGCTCGCTGGCTGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCG
GTGCTTCTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCT
GGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTTAGCAACCCTTCCGCCATCTTTTTAAGCCGCGGTGATGAAG
TGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAACGCGTCCACTGCTGGTGGAGATCCAGGCATTGGTCGATCAC
TCGATGATGGCGAATCCACGCCGCGTCGCAGTAGGGCTGGAGCAAAACCGTCTGGCAATCCTGCTGGCGGTGCTGCACCG
TCACGGTGGGTTGCAAATGGCCGATCAGGATGTATTTGTTAACGTGGTCGGCGGCGTGAAGGTGACGGAAACCAGTGCCG
ACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGATAGACCGCTACCGCAGGATCTGGTGGTGTTTGGTGAAGTC
GGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGCCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGGCGGGC
GATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGC
TTAGCGTGTTCGACGACTTATAA

Domains

Predicted by InterProScan.

AAA domain (AAA_25)

(89-225)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(348-433)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2T3RLH6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA/sms Bacillus subtilis subsp. subtilis str. 168

46.827

99.348

0.465
  radA Streptococcus mitis SK321

43.86

99.13

0.435
  radA Streptococcus pneumoniae D39

43.421

99.13

0.43
  radA Streptococcus pneumoniae TIGR4

43.421

99.13

0.43
  radA Streptococcus mitis NCTC 12261

43.421

99.13

0.43
  radA Streptococcus pneumoniae R6

43.421

99.13

0.43
  radA Streptococcus pneumoniae Rx1

43.421

99.13

0.43