Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACHP5I_RS03085 Genome accession   NZ_CP172185
Coordinates   642783..644216 (-) Length   477 a.a.
NCBI ID   WP_074518354.1    Uniprot ID   -
Organism   Escherichia coli strain GF60     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 637783..649216
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHP5I_RS03050 (ACHP5I_03050) elbB 638475..639128 (+) 654 WP_021564672.1 isoprenoid biosynthesis glyoxalase ElbB -
  ACHP5I_RS03055 (ACHP5I_03055) mtgA 639125..639853 (+) 729 WP_021564671.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  ACHP5I_RS03060 (ACHP5I_03060) yrbL 639850..640482 (-) 633 WP_000620420.1 PhoP regulatory network protein YrbL -
  ACHP5I_RS03065 (ACHP5I_03065) npr 640695..640967 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  ACHP5I_RS03070 (ACHP5I_03070) rapZ 640964..641818 (-) 855 WP_000243741.1 RNase adapter RapZ -
  ACHP5I_RS03075 (ACHP5I_03075) ptsN 641864..642355 (-) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACHP5I_RS03080 (ACHP5I_03080) hpf 642473..642760 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  ACHP5I_RS03085 (ACHP5I_03085) rpoN/rpoN1 642783..644216 (-) 1434 WP_074518354.1 RNA polymerase factor sigma-54 Machinery gene
  ACHP5I_RS03090 (ACHP5I_03090) lptB 644264..644989 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  ACHP5I_RS03095 (ACHP5I_03095) lptA 644996..645553 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  ACHP5I_RS03100 (ACHP5I_03100) lptC 645522..646097 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  ACHP5I_RS03105 (ACHP5I_03105) kdsC 646094..646660 (-) 567 WP_000030005.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ACHP5I_RS03110 (ACHP5I_03110) kdsD 646681..647667 (-) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  ACHP5I_RS03115 (ACHP5I_03115) yrbG 647681..648658 (-) 978 WP_000922872.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54027.81 Da        Isoelectric Point: 4.3639

>NTDB_id=950944 ACHP5I_RS03085 WP_074518354.1 642783..644216(-) (rpoN/rpoN1) [Escherichia coli strain GF60]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADAFEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=950944 ACHP5I_RS03085 WP_074518354.1 642783..644216(-) (rpoN/rpoN1) [Escherichia coli strain GF60]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGTTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGTTGACACCGTTTT
CTGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCACTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATTGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCAGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAAACCACGCCATGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACGGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGCGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465