Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACHP5M_RS19540 Genome accession   NZ_CP172163
Coordinates   4031345..4032778 (+) Length   477 a.a.
NCBI ID   WP_000809047.1    Uniprot ID   -
Organism   Escherichia coli strain GF50     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4026345..4037778
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHP5M_RS19510 (ACHP5M_19510) yrbG 4026903..4027880 (+) 978 WP_000922872.1 calcium/sodium antiporter -
  ACHP5M_RS19515 (ACHP5M_19515) kdsD 4027894..4028880 (+) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  ACHP5M_RS19520 (ACHP5M_19520) kdsC 4028901..4029467 (+) 567 WP_000030005.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ACHP5M_RS19525 (ACHP5M_19525) lptC 4029464..4030039 (+) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  ACHP5M_RS19530 (ACHP5M_19530) lptA 4030008..4030565 (+) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  ACHP5M_RS19535 (ACHP5M_19535) lptB 4030572..4031297 (+) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  ACHP5M_RS19540 (ACHP5M_19540) rpoN/rpoN1 4031345..4032778 (+) 1434 WP_000809047.1 RNA polymerase factor sigma-54 Machinery gene
  ACHP5M_RS19545 (ACHP5M_19545) hpf 4032801..4033088 (+) 288 WP_001176599.1 ribosome hibernation promoting factor -
  ACHP5M_RS19550 (ACHP5M_19550) ptsN 4033206..4033697 (+) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACHP5M_RS19555 (ACHP5M_19555) rapZ 4033743..4034597 (+) 855 WP_000243741.1 RNase adapter RapZ -
  ACHP5M_RS19560 (ACHP5M_19560) npr 4034594..4034866 (+) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  ACHP5M_RS19565 (ACHP5M_19565) yrbL 4035080..4035712 (+) 633 WP_000620405.1 PhoP regulatory network protein YrbL -
  ACHP5M_RS19570 (ACHP5M_19570) mtgA 4035709..4036437 (-) 729 WP_000047091.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  ACHP5M_RS19575 (ACHP5M_19575) elbB 4036434..4037087 (-) 654 WP_001299134.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54051.83 Da        Isoelectric Point: 4.3447

>NTDB_id=950667 ACHP5M_RS19540 WP_000809047.1 4031345..4032778(+) (rpoN/rpoN1) [Escherichia coli strain GF50]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDDDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=950667 ACHP5M_RS19540 WP_000809047.1 4031345..4032778(+) (rpoN/rpoN1) [Escherichia coli strain GF50]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
TGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGACACTGATCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAGGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTAGCGGCAAAAGATCTGCGTGACTGCCTGCTAATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGATGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCACTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTACTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465