Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   WHL51_RS12815 Genome accession   NZ_CP148102
Coordinates   2502891..2503508 (-) Length   205 a.a.
NCBI ID   WP_003226145.1    Uniprot ID   -
Organism   Bacillus spizizenii str. W23     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2497891..2508508
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WHL51_RS12785 rpsT 2498192..2498458 (+) 267 WP_003226155.1 30S ribosomal protein S20 -
  WHL51_RS12790 holA 2498473..2499516 (-) 1044 WP_003226154.1 DNA polymerase III subunit delta -
  WHL51_RS12795 - 2499556..2499705 (-) 150 WP_136975859.1 hypothetical protein -
  WHL51_RS12800 - 2499746..2499880 (+) 135 WP_003226152.1 YqzM family protein -
  WHL51_RS12805 comEC 2499921..2502251 (-) 2331 WP_003226149.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  WHL51_RS12810 - 2502255..2502824 (-) 570 WP_003226147.1 ComE operon protein 2 -
  WHL51_RS12815 comEA 2502891..2503508 (-) 618 WP_003226145.1 helix-hairpin-helix domain-containing protein Machinery gene
  WHL51_RS12820 comER 2503592..2504413 (+) 822 WP_003226143.1 late competence protein ComER -
  WHL51_RS12825 - 2504479..2505222 (-) 744 WP_003226141.1 class I SAM-dependent methyltransferase -
  WHL51_RS12830 rsfS 2505219..2505575 (-) 357 WP_003226139.1 ribosome silencing factor -
  WHL51_RS12835 yqeK 2505593..2506153 (-) 561 WP_003226137.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  WHL51_RS12840 - 2506143..2506712 (-) 570 WP_003226135.1 nicotinate-nucleotide adenylyltransferase -
  WHL51_RS12845 yhbY 2506724..2507014 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  WHL51_RS12850 aroE 2507008..2507850 (-) 843 WP_003226130.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21919.78 Da        Isoelectric Point: 5.1470

>NTDB_id=950443 WHL51_RS12815 WP_003226145.1 2502891..2503508(-) (comEA) [Bacillus spizizenii str. W23]
MNWLNHHKKAIILGASAAVFIAIIFFLASGKNKESMKQTVSTETENTEVKQEAVKDESNETIVIDIKGAVKHPGVYEMRT
GDRLSQAIEKAGGTSEQADEMQVNLAGLLQDGTVVYIPKRGEETAVQQGVGGAIQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=950443 WHL51_RS12815 WP_003226145.1 2502891..2503508(-) (comEA) [Bacillus spizizenii str. W23]
ATGAATTGGTTGAATCATCATAAGAAAGCAATTATTTTAGGGGCCTCTGCGGCTGTTTTCATAGCGATCATTTTCTTCCT
GGCCAGCGGGAAAAACAAAGAGTCGATGAAGCAGACTGTATCAACTGAGACAGAAAATACGGAGGTAAAGCAGGAAGCAG
TCAAAGATGAGTCAAACGAAACGATTGTGATTGACATCAAAGGTGCTGTTAAGCACCCTGGTGTTTATGAAATGCGAACA
GGGGACAGATTATCCCAGGCAATTGAGAAAGCCGGCGGGACCAGTGAACAAGCAGACGAAATGCAAGTGAATTTGGCGGG
GCTGCTGCAGGACGGGACAGTGGTGTACATCCCGAAGAGGGGTGAGGAGACAGCGGTGCAGCAAGGAGTCGGAGGCGCCA
TCCAAAGCGATGGAGGAAAGGGGGCGCTGGTAAATATCAATACAGCAACCTTAGAGGAATTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAATGGGCGTTTCCAGACAATTGAAGATATTACGAAGGT
TTCAGGGATCGGTGAAAAGTCGTTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

89.268

100

0.893
  comEA Latilactobacillus sakei subsp. sakei 23K

31.646

100

0.366
  comEA Staphylococcus aureus MW2

37.688

97.073

0.366