Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACHP5P_RS02690 Genome accession   NZ_CP172145
Coordinates   554449..555882 (-) Length   477 a.a.
NCBI ID   WP_395763276.1    Uniprot ID   -
Organism   Escherichia coli strain GF66.2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 549449..560882
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHP5P_RS02655 (ACHP5P_02655) elbB 550140..550793 (+) 654 WP_001300411.1 isoprenoid biosynthesis glyoxalase ElbB -
  ACHP5P_RS02660 (ACHP5P_02660) mtgA 550790..551518 (+) 729 WP_000047091.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  ACHP5P_RS02665 (ACHP5P_02665) yrbL 551515..552147 (-) 633 WP_000620405.1 PhoP regulatory network protein YrbL -
  ACHP5P_RS02670 (ACHP5P_02670) npr 552361..552633 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  ACHP5P_RS02675 (ACHP5P_02675) rapZ 552630..553484 (-) 855 WP_000243741.1 RNase adapter RapZ -
  ACHP5P_RS02680 (ACHP5P_02680) ptsN 553530..554021 (-) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACHP5P_RS02685 (ACHP5P_02685) hpf 554139..554426 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  ACHP5P_RS02690 (ACHP5P_02690) rpoN/rpoN1 554449..555882 (-) 1434 WP_395763276.1 RNA polymerase factor sigma-54 Machinery gene
  ACHP5P_RS02695 (ACHP5P_02695) lptB 555930..556655 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  ACHP5P_RS02700 (ACHP5P_02700) lptA 556662..557219 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  ACHP5P_RS02705 (ACHP5P_02705) lptC 557188..557763 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  ACHP5P_RS02710 (ACHP5P_02710) kdsC 557760..558326 (-) 567 WP_000030005.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ACHP5P_RS02715 (ACHP5P_02715) kdsD 558347..559333 (-) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  ACHP5P_RS02720 (ACHP5P_02720) yrbG 559347..560324 (-) 978 WP_000922901.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54021.84 Da        Isoelectric Point: 4.3639

>NTDB_id=950384 ACHP5P_RS02690 WP_395763276.1 554449..555882(-) (rpoN/rpoN1) [Escherichia coli strain GF66.2]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIVAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=950384 ACHP5P_RS02690 WP_395763276.1 554449..555882(-) (rpoN/rpoN1) [Escherichia coli strain GF66.2]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
TGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAGGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTAGCGGCAAAAGATCTGCGTGACTGCCTGCTAATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGATTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACAGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCTCGCAATGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGCGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGTGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAACTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465