Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACHP5Q_RS17505 Genome accession   NZ_CP172140
Coordinates   3604867..3606300 (-) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain GF130     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3599867..3611300
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHP5Q_RS17470 (ACHP5Q_17470) elbB 3600559..3601212 (+) 654 WP_000719791.1 isoprenoid biosynthesis glyoxalase ElbB -
  ACHP5Q_RS17475 (ACHP5Q_17475) mtgA 3601209..3601937 (+) 729 WP_000047069.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  ACHP5Q_RS17480 (ACHP5Q_17480) yrbL 3601934..3602566 (-) 633 WP_097751903.1 PhoP regulatory network protein YrbL -
  ACHP5Q_RS17485 (ACHP5Q_17485) npr 3602779..3603051 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  ACHP5Q_RS17490 (ACHP5Q_17490) rapZ 3603048..3603902 (-) 855 WP_000243741.1 RNase adapter RapZ -
  ACHP5Q_RS17495 (ACHP5Q_17495) ptsN 3603948..3604439 (-) 492 WP_000183674.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACHP5Q_RS17500 (ACHP5Q_17500) hpf 3604557..3604844 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  ACHP5Q_RS17505 (ACHP5Q_17505) rpoN/rpoN1 3604867..3606300 (-) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  ACHP5Q_RS17510 (ACHP5Q_17510) lptB 3606348..3607073 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  ACHP5Q_RS17515 (ACHP5Q_17515) lptA 3607080..3607637 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  ACHP5Q_RS17520 (ACHP5Q_17520) lptC 3607606..3608181 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  ACHP5Q_RS17525 (ACHP5Q_17525) kdsC 3608178..3608744 (-) 567 WP_000030005.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ACHP5Q_RS17530 (ACHP5Q_17530) kdsD 3608765..3609751 (-) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  ACHP5Q_RS17535 (ACHP5Q_17535) yrbG 3609765..3610742 (-) 978 WP_000922880.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=950350 ACHP5Q_RS17505 WP_000809051.1 3604867..3606300(-) (rpoN/rpoN1) [Escherichia coli strain GF130]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=950350 ACHP5Q_RS17505 WP_000809051.1 3604867..3606300(-) (rpoN/rpoN1) [Escherichia coli strain GF130]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACACTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTATCAGGGCGAAACGACACAGACCTTACAGGATTACCTGATGTGGCAGGTCGAGTTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATTGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGCCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCATGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTATTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGATAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGCGAAGAATATATGAAACCGATGGTACTGGCTGATATCGCCCAGGCCG
TCGAGATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAA
TATTTCTTTTCCAGTCACGTCAATACTGAAGGCGGGGGTGAAGCCTCTTCCACAGCGATTCGCGCACTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCATTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CTCGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465