Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA/sms   Type   Machinery gene
Locus tag   ACHP5S_RS12030 Genome accession   NZ_CP172127
Coordinates   2458696..2460078 (-) Length   460 a.a.
NCBI ID   WP_001029698.1    Uniprot ID   A0A2T3RLH6
Organism   Escherichia coli strain GF43     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2453696..2465078
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHP5S_RS11990 (ACHP5S_11990) - 2453995..2454792 (+) 798 WP_000762714.1 SDR family NAD(P)-dependent oxidoreductase -
  ACHP5S_RS11995 (ACHP5S_11995) - 2454818..2455234 (+) 417 WP_001140838.1 SRPBCC family protein -
  ACHP5S_RS12000 (ACHP5S_12000) - 2455392..2455604 (+) 213 WP_000848792.1 DUF1471 domain-containing protein -
  ACHP5S_RS12005 (ACHP5S_12005) - 2455677..2455793 (+) 117 Protein_2359 hypothetical protein -
  ACHP5S_RS12010 (ACHP5S_12010) - 2455917..2456677 (+) 761 Protein_2360 ATP-binding cassette domain-containing protein -
  ACHP5S_RS12015 (ACHP5S_12015) nadS 2456733..2457017 (-) 285 WP_000007436.1 NadS family protein -
  ACHP5S_RS12020 (ACHP5S_12020) - 2457019..2457351 (-) 333 WP_000513551.1 type II toxin-antitoxin system RelE/ParE family toxin -
  ACHP5S_RS12025 (ACHP5S_12025) nadR 2457443..2458675 (-) 1233 WP_000093812.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  ACHP5S_RS12030 (ACHP5S_12030) radA/sms 2458696..2460078 (-) 1383 WP_001029698.1 DNA repair protein RadA Machinery gene
  ACHP5S_RS12035 (ACHP5S_12035) serB 2460127..2461095 (-) 969 WP_001132956.1 phosphoserine phosphatase -
  ACHP5S_RS12040 (ACHP5S_12040) ytjB 2461201..2461845 (+) 645 WP_000124615.1 YtjB family periplasmic protein -
  ACHP5S_RS12045 (ACHP5S_12045) lplA 2461873..2462889 (+) 1017 WP_042003971.1 lipoate--protein ligase LplA -
  ACHP5S_RS12050 (ACHP5S_12050) yjjJ 2462890..2464221 (-) 1332 WP_042003972.1 type II toxin-antitoxin system HipA family toxin YjjJ -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49442.02 Da        Isoelectric Point: 7.1936

>NTDB_id=950146 ACHP5S_RS12030 WP_001029698.1 2458696..2460078(-) (radA/sms) [Escherichia coli strain GF43]
MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPTVARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTG
FKEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDH
SMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQDLVVFGEV
GLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKLSDALSVFDDL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=950146 ACHP5S_RS12030 WP_001029698.1 2458696..2460078(-) (radA/sms) [Escherichia coli strain GF43]
GTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGACAGTGCAGTGC
CTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCCGCGTCACCAACGGTGGCGCGTAACGAGCGTCTCAGTGGCT
ATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTTTCCGACATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGA
TTTAAAGAGTTCGACCGCGTATTAGGCGGCGGGGTGGTGCCGGGAAGCGCCATTCTGATTGGCGGTAACCCAGGTGCGGG
GAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCGCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGC
TGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAG
CAAATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCCATTCAGGTGATGCATATGGCGGATGT
ACAATCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGCGTGG
CGATTGTCATGGTTGGGCACGTAACCAAAGATGGTTCGCTGGCTGGCCCTAAAGTGCTGGAACACTGTATTGACTGTTCG
GTGCTTCTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCT
GGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCCGCCATCTTTTTAAGCCGCGGTGATGAAG
TGACCTCCGGTAGCTCAGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCAC
TCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCG
TCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCG
ACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTC
GGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGC
GATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGC
TTAGCGTGTTCGACGACTTATAA

Domains

Predicted by InterProScan.

AAA domain (AAA_25)

(89-225)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(348-433)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2T3RLH6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA/sms Bacillus subtilis subsp. subtilis str. 168

46.827

99.348

0.465
  radA Streptococcus mitis SK321

43.86

99.13

0.435
  radA Streptococcus pneumoniae D39

43.421

99.13

0.43
  radA Streptococcus pneumoniae TIGR4

43.421

99.13

0.43
  radA Streptococcus mitis NCTC 12261

43.421

99.13

0.43
  radA Streptococcus pneumoniae R6

43.421

99.13

0.43
  radA Streptococcus pneumoniae Rx1

43.421

99.13

0.43