NTDB

Overview

Name	radA	Type	Machinery gene
Locus tag	WHF41_RS02425	Genome accession	NZ_CP148032
Coordinates	479991..481331 (+)	Length	446 a.a.
NCBI ID	WP_019225188.1	Uniprot ID	A0A1C2Y1Z7
Organism	MAG: Dehalobacter restrictus isolate SAD
Function	homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 474991..486331

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WHF41_RS02405 (WHF41_02390)	-	475386..475859 (+)	474	WP_019225184.1	CtsR family transcriptional regulator	-
WHF41_RS02410 (WHF41_02395)	-	475874..476398 (+)	525	WP_019225185.1	UvrB/UvrC motif-containing protein	-
WHF41_RS02415 (WHF41_02400)	-	476395..477462 (+)	1068	WP_019225186.1	protein arginine kinase	-
WHF41_RS02420 (WHF41_02405)	-	477494..479977 (+)	2484	WP_019225187.1	ATP-dependent Clp protease ATP-binding subunit	-
WHF41_RS02425 (WHF41_02410)	radA	479991..481331 (+)	1341	WP_019225188.1	DNA repair protein RadA	Machinery gene
WHF41_RS02430 (WHF41_02415)	-	481485..481877 (-)	393	WP_019225189.1	hypothetical protein	-
WHF41_RS02435 (WHF41_02420)	-	482148..483314 (+)	1167	WP_019225190.1	PIN/TRAM domain-containing protein	-
WHF41_RS02440 (WHF41_02425)	ispD	483292..484479 (+)	1188	WP_019225191.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-
WHF41_RS02445 (WHF41_02430)	gltX	484587..486044 (+)	1458	WP_019225192.1	glutamate--tRNA ligase	-

Sequence

Protein

Download Length: 446 a.a. Molecular weight: 48840.18 Da Isoelectric Point: 5.6956

                                >NTDB_id=950112 WHF41_RS02425 WP_019225188.1 479991..481331(+) (radA) [MAG: Dehalobacter restrictus isolate SAD]

MAGPKTKFYCRECGQESARWLGRCPGCGEWNTLIEERVEKSKPTEHRGIVQAIPLTEIQTMEGQRLDTGSPELNRVFGGG

VVEGSFVLLSGEPGIGKSTLFLQMAEYLSRKENVLYVSGEESARQIKLRADRMELSSSRVHILADNSLEAVRSEVLNKGY

KVVFIDSIQTMLLEDVQSAPGSVSQVREGASFLLKLAKENEITVFLAGHITKEGAIAGPRVLEHMVDTVLYFEGDQHHIF

RLLRAVKNRFGPANEIGVFEMRGCGLADVTNPSMFFMGDHTQVSAGSGVAVVMEGTRPLLVEVQALVTSSIFAPPRRTVN

GMDYHRLLMLLAVLDKRAGYAFGTRDVFVNIAGGLDVDEPAADLAVIASVMSGIKDQPLGNMALIGELGLTGEIRGVSHI

EQRIREAEKFGFQNCLVPKVNADSIGKTDCRIIPVKNIEEVLDFLF

Nucleotide

Download Length: 1341 bp

                                >NTDB_id=950112 WHF41_RS02425 WP_019225188.1 479991..481331(+) (radA) [MAG: Dehalobacter restrictus isolate SAD]

TTGGCAGGTCCGAAGACGAAGTTCTATTGCCGGGAATGCGGGCAGGAAAGCGCGAGATGGCTTGGAAGGTGTCCCGGATG

CGGTGAATGGAATACACTGATTGAGGAGCGCGTAGAAAAATCCAAACCTACAGAACACAGAGGGATCGTTCAGGCGATCC

CTCTGACTGAAATCCAAACGATGGAAGGGCAGAGACTTGATACCGGAAGTCCGGAACTTAACCGGGTTTTCGGGGGAGGG

GTTGTCGAAGGGTCCTTTGTTCTATTAAGCGGGGAGCCCGGGATTGGCAAATCCACTTTGTTTCTGCAAATGGCAGAATA

TCTTTCCCGGAAGGAAAATGTGCTTTATGTGTCGGGAGAGGAATCTGCCCGGCAAATCAAACTCAGGGCTGACAGAATGG

AGCTTTCTTCTTCCCGGGTACATATACTCGCCGATAATTCCCTGGAAGCAGTACGTTCGGAGGTGCTGAACAAAGGCTAT

AAAGTCGTTTTTATTGATTCGATCCAGACAATGCTGCTCGAAGACGTTCAGTCTGCACCCGGAAGCGTCAGCCAGGTAAG

GGAAGGGGCTTCTTTTTTGCTGAAACTGGCCAAAGAAAATGAGATTACGGTTTTCCTGGCCGGCCATATTACAAAGGAGG

GTGCGATTGCCGGACCCAGGGTTCTGGAGCACATGGTCGATACGGTCCTGTATTTTGAAGGAGATCAGCACCATATTTTC

CGTCTGCTGCGGGCAGTTAAAAACCGTTTTGGTCCGGCCAACGAAATTGGGGTATTTGAGATGAGAGGCTGCGGTCTAGC

TGATGTTACAAATCCCTCCATGTTTTTTATGGGCGATCATACACAGGTATCTGCTGGTTCCGGGGTGGCTGTCGTGATGG

AAGGAACAAGGCCGCTCTTAGTGGAAGTCCAGGCTTTGGTAACATCGTCCATCTTTGCGCCGCCGAGAAGAACCGTAAAC

GGGATGGATTACCACCGCCTGCTGATGCTTCTGGCAGTCCTGGACAAACGAGCCGGGTACGCTTTTGGCACCCGGGATGT

TTTTGTCAATATTGCCGGGGGTCTCGATGTGGATGAACCGGCAGCGGATCTTGCCGTTATTGCCTCTGTGATGTCGGGAA

TTAAAGATCAGCCGCTTGGAAATATGGCTTTAATCGGGGAACTCGGTTTGACCGGAGAAATTAGAGGGGTTTCTCATATT

GAGCAGCGGATCAGAGAAGCGGAAAAATTTGGTTTCCAAAATTGCCTGGTACCAAAAGTCAATGCAGACAGTATCGGGAA

GACCGATTGCCGAATCATTCCGGTTAAGAACATTGAGGAAGTACTGGATTTTCTTTTTTAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(64-214)

Subunit ChlI of Mg-chelatase (ChlI)

(337-421)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A1C2Y1Z7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
radA	Bacillus subtilis subsp. subtilis str. 168	50.552	100	0.513
radA	Streptococcus mitis NCTC 12261	48.59	100	0.502
radA	Streptococcus pneumoniae Rx1	48.59	100	0.502
radA	Streptococcus pneumoniae D39	48.59	100	0.502
radA	Streptococcus pneumoniae R6	48.59	100	0.502
radA	Streptococcus pneumoniae TIGR4	48.59	100	0.502
radA	Streptococcus mitis SK321	48.59	100	0.502

Detailed information NTG0950112