Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WGR13_RS20340 Genome accession   NZ_CP148011
Coordinates   4216252..4217685 (+) Length   477 a.a.
NCBI ID   WP_023295498.1    Uniprot ID   -
Organism   Enterobacter hormaechei strain FUJ80021     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4211252..4222685
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WGR13_RS20310 (WGR13_20295) - 4211819..4212796 (+) 978 WP_003861852.1 calcium/sodium antiporter -
  WGR13_RS20315 (WGR13_20300) kdsD 4212810..4213796 (+) 987 WP_003861853.1 arabinose-5-phosphate isomerase KdsD -
  WGR13_RS20320 (WGR13_20305) kdsC 4213811..4214377 (+) 567 WP_017693909.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  WGR13_RS20325 (WGR13_20310) lptC 4214374..4214949 (+) 576 WP_015571983.1 LPS export ABC transporter periplasmic protein LptC -
  WGR13_RS20330 (WGR13_20315) lptA 4214918..4215472 (+) 555 WP_006812136.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  WGR13_RS20335 (WGR13_20320) lptB 4215479..4216204 (+) 726 WP_003861862.1 LPS export ABC transporter ATP-binding protein -
  WGR13_RS20340 (WGR13_20325) rpoN/rpoN1 4216252..4217685 (+) 1434 WP_023295498.1 RNA polymerase factor sigma-54 Machinery gene
  WGR13_RS20345 (WGR13_20330) hpf 4217708..4217995 (+) 288 WP_003861866.1 ribosome hibernation promoting factor -
  WGR13_RS20350 (WGR13_20335) ptsN 4218078..4218569 (+) 492 WP_003861869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  WGR13_RS20355 (WGR13_20340) rapZ 4218615..4219469 (+) 855 WP_003861870.1 RNase adapter RapZ -
  WGR13_RS20360 (WGR13_20345) npr 4219466..4219738 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  WGR13_RS20365 (WGR13_20350) mtgA 4219778..4220503 (-) 726 WP_023295499.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  WGR13_RS20370 (WGR13_20355) elbB 4220500..4221153 (-) 654 WP_015571987.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54098.98 Da        Isoelectric Point: 4.3549

>NTDB_id=950075 WGR13_RS20340 WP_023295498.1 4216252..4217685(+) (rpoN/rpoN1) [Enterobacter hormaechei strain FUJ80021]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDAQQTQDTETLDSVDALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDE
ILESIGNDEIELEEIEAVLKRVQRFDPIGVAAKDLRDCLLIQLSQFAKETPWIDEARLIISDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGRWVVELNADSIPRLQINQQYASMCTSARNDADNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=950075 WGR13_RS20340 WP_023295498.1 4216252..4217685(+) (rpoN/rpoN1) [Enterobacter hormaechei strain FUJ80021]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAGCAACTGGCCATGACGCCGCAGCTACAGCAGGCAATCCGTCTGTTGCA
GCTGTCCACGTTAGAACTCCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAATCCGCTGCTGGAGCAAACCGATCTTCATG
ACGAGGTAGACGCCCAGCAGACGCAGGACACAGAAACGCTCGACTCCGTAGATGCGCTCGAACAGAAAGAGATGCCGGAC
GAACTGCCGCTGGATGCCAGCTGGGATGAAATTTACACCGCGGGTACCCCTTCCGGGACACGTGCAGACTACCAGGACGA
TGAACTCCCGGTCTACCAGGGCGAAACCACCCAGTCGCTTCAGGACTACCTGATGTGGCAGGTAGAGCTGACCCCTTTCT
CCGATACCGATCGCGCCATTGCCACCTCGATTGTCGATGCCGTTGACGACACGGGCTATTTGACCGTGACGTTGGACGAG
ATTCTTGAAAGCATCGGCAATGACGAGATTGAACTGGAAGAGATCGAAGCCGTTCTGAAACGCGTTCAGCGTTTCGACCC
GATAGGTGTCGCCGCGAAAGATCTGCGCGACTGCCTTCTGATCCAGCTTTCGCAGTTTGCCAAAGAGACACCGTGGATTG
ACGAAGCCCGGCTCATCATCAGCGATCATCTGGATCTGCTGGCGAATCACGACTTCCGCACCCTGATGCGCGTCACACGC
CTGAAAGAAGAGGTGCTGAAGGAAGCGGTGAATCTGATCCAGTCGCTCGATCCGCGTCCGGGGCAGTCGATCCAGACCAG
CGAACCGGAATATGTCATTCCTGATGTGCTGGTGAGAAAACACAATGGCCGGTGGGTCGTTGAACTGAACGCCGACAGCA
TTCCTCGCCTGCAAATCAACCAGCAATACGCCTCCATGTGCACCAGCGCCCGTAACGACGCTGATAATCAATACATTCGC
AGCAACCTTCAGGAAGCACGCTGGTTGATCAAGAGCCTGGAGAGCCGTAATGACACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTTTATGAAACCGATGGTGCTGGCAGACATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAATATCTGCACAGCCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCACGTGAACACCGAAGGCGGCGGCGAAGCCTCGTCAACGGCTATTCGCGCACTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTCAGTGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTCGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAACTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48