Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACHMKG_RS02850 Genome accession   NZ_CP171874
Coordinates   585315..586748 (-) Length   477 a.a.
NCBI ID   WP_096313105.1    Uniprot ID   -
Organism   Escherichia coli strain YZ22PE67     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 580315..591748
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHMKG_RS02815 elbB 581006..581659 (+) 654 WP_001300411.1 isoprenoid biosynthesis glyoxalase ElbB -
  ACHMKG_RS02820 mtgA 581656..582384 (+) 729 WP_000047091.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  ACHMKG_RS02825 yrbL 582381..583013 (-) 633 WP_000620405.1 PhoP regulatory network protein YrbL -
  ACHMKG_RS02830 npr 583227..583499 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  ACHMKG_RS02835 rapZ 583496..584350 (-) 855 WP_000243741.1 RNase adapter RapZ -
  ACHMKG_RS02840 ptsN 584396..584887 (-) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACHMKG_RS02845 hpf 585005..585292 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  ACHMKG_RS02850 rpoN/rpoN1 585315..586748 (-) 1434 WP_096313105.1 RNA polymerase factor sigma-54 Machinery gene
  ACHMKG_RS02855 lptB 586796..587521 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  ACHMKG_RS02860 lptA 587528..588085 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  ACHMKG_RS02865 lptC 588054..588629 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  ACHMKG_RS02870 kdsC 588626..589192 (-) 567 WP_000030005.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ACHMKG_RS02875 kdsD 589213..590199 (-) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  ACHMKG_RS02880 yrbG 590213..591190 (-) 978 WP_000922901.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54019.87 Da        Isoelectric Point: 4.3663

>NTDB_id=949580 ACHMKG_RS02850 WP_096313105.1 585315..586748(-) (rpoN/rpoN1) [Escherichia coli strain YZ22PE67]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETMQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
ILESIGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=949580 ACHMKG_RS02850 WP_096313105.1 585315..586748(-) (rpoN/rpoN1) [Escherichia coli strain YZ22PE67]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTACCAGGGCGAAACGATGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAACTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.024

100

0.463