Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACHMKI_RS19265 Genome accession   NZ_CP171864
Coordinates   3991480..3992913 (+) Length   477 a.a.
NCBI ID   WP_096313105.1    Uniprot ID   -
Organism   Escherichia coli strain YZ22PE106     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3986480..3997913
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHMKI_RS19235 yrbG 3987038..3988015 (+) 978 WP_000922901.1 calcium/sodium antiporter -
  ACHMKI_RS19240 kdsD 3988029..3989015 (+) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  ACHMKI_RS19245 kdsC 3989036..3989602 (+) 567 WP_000030005.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ACHMKI_RS19250 lptC 3989599..3990174 (+) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  ACHMKI_RS19255 lptA 3990143..3990700 (+) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  ACHMKI_RS19260 lptB 3990707..3991432 (+) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  ACHMKI_RS19265 rpoN/rpoN1 3991480..3992913 (+) 1434 WP_096313105.1 RNA polymerase factor sigma-54 Machinery gene
  ACHMKI_RS19270 hpf 3992936..3993223 (+) 288 WP_001176599.1 ribosome hibernation promoting factor -
  ACHMKI_RS19275 ptsN 3993341..3993832 (+) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACHMKI_RS19280 rapZ 3993878..3994732 (+) 855 WP_000243741.1 RNase adapter RapZ -
  ACHMKI_RS19285 npr 3994729..3995001 (+) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  ACHMKI_RS19290 yrbL 3995215..3995847 (+) 633 WP_000620405.1 PhoP regulatory network protein YrbL -
  ACHMKI_RS19295 mtgA 3995844..3996572 (-) 729 WP_000047091.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  ACHMKI_RS19300 elbB 3996569..3997222 (-) 654 WP_001300411.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54019.87 Da        Isoelectric Point: 4.3663

>NTDB_id=949458 ACHMKI_RS19265 WP_096313105.1 3991480..3992913(+) (rpoN/rpoN1) [Escherichia coli strain YZ22PE106]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETMQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
ILESIGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=949458 ACHMKI_RS19265 WP_096313105.1 3991480..3992913(+) (rpoN/rpoN1) [Escherichia coli strain YZ22PE106]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTACCAGGGCGAAACGATGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAACTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.024

100

0.463