Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   WFE12_RS02000 Genome accession   NZ_CP147877
Coordinates   373574..374023 (-) Length   149 a.a.
NCBI ID   WP_003246436.1    Uniprot ID   A0AAE2SFQ0
Organism   Bacillus subtilis subsp. subtilis str. 168     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 368574..379023
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WFE12_RS01965 yckA 368725..369405 (-) 681 WP_003234624.1 amino acid ABC transporter permease -
  WFE12_RS01970 yckB 369415..370278 (-) 864 WP_009966510.1 transporter substrate-binding domain-containing protein -
  WFE12_RS01975 - 370440..370637 (+) 198 WP_119122841.1 hypothetical protein -
  WFE12_RS01980 yckC 370656..371111 (+) 456 WP_003246356.1 RDD family protein -
  WFE12_RS01985 yckD 371193..371525 (+) 333 WP_003246247.1 YckD family protein -
  WFE12_RS01990 bglC 371679..373112 (+) 1434 WP_003246242.1 6-phospho-beta-glucosidase -
  WFE12_RS01995 nin/comJ 373149..373547 (-) 399 WP_003246342.1 DNA-entry nuclease inhibitor Regulator
  WFE12_RS02000 nucA/comI 373574..374023 (-) 450 WP_003246436.1 DNA-entry nuclease Machinery gene
  WFE12_RS02005 tlpC 374191..375912 (-) 1722 WP_003246498.1 methyl-accepting chemotaxis protein TlpC -
  WFE12_RS02010 hxlB 376023..376580 (-) 558 WP_003246343.1 6-phospho-3-hexuloisomerase -
  WFE12_RS02015 hxlA 376586..377218 (-) 633 WP_003246452.1 3-hexulose-6-phosphate synthase -
  WFE12_RS02020 hxlR 377451..377813 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16486.48 Da        Isoelectric Point: 4.6274

>NTDB_id=949107 WFE12_RS02000 WP_003246436.1 373574..374023(-) (nucA/comI) [Bacillus subtilis subsp. subtilis str. 168]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETMAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHRLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=949107 WFE12_RS02000 WP_003246436.1 373574..374023(-) (nucA/comI) [Bacillus subtilis subsp. subtilis str. 168]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATGGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAAGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
GGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(53-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

100

100

1