Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   WDW95_RS09530 Genome accession   NZ_CP147857
Coordinates   1922856..1924217 (-) Length   453 a.a.
NCBI ID   WP_368651520.1    Uniprot ID   -
Organism   Lactococcus raffinolactis strain APC3967     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1917856..1929217
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WDW95_RS09505 gltX 1917998..1919446 (-) 1449 WP_096039569.1 glutamate--tRNA ligase -
  WDW95_RS09510 ispF 1919591..1920073 (-) 483 WP_096039570.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  WDW95_RS09515 - 1920060..1920821 (-) 762 WP_061774662.1 TIGR00266 family protein -
  WDW95_RS09520 ispD 1920877..1921557 (-) 681 WP_096039571.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  WDW95_RS09525 - 1921554..1922627 (-) 1074 WP_096039572.1 PIN/TRAM domain-containing protein -
  WDW95_RS09530 radA 1922856..1924217 (-) 1362 WP_368651520.1 DNA repair protein RadA Machinery gene
  WDW95_RS09535 - 1924219..1924665 (-) 447 WP_061774658.1 dUTP diphosphatase -
  WDW95_RS09540 - 1924748..1926499 (-) 1752 WP_167839076.1 ABC transporter ATP-binding protein -
  WDW95_RS09545 - 1926492..1928210 (-) 1719 WP_167839077.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49356.85 Da        Isoelectric Point: 5.4218

>NTDB_id=949009 WDW95_RS09530 WP_368651520.1 1922856..1924217(-) (radA) [Lactococcus raffinolactis strain APC3967]
MAKAKSKFVCQSCGYQSPKYLGRCPNCGAWNSFVEEIEAKEVKNARVTLTGEKTRPVKLGEVESLDTPRVLTDMGEFNRV
LGGGVVPGSLILIGGDPGIGKSTILLQVSVQLANLGRVLYVSGEESAQQIKMRAERLGDLDSDFYLYTENNMQNIRAEIE
KLQPDFLVIDSIQTVMSPEITGVQGSVSQVREVTAELLQIAKTNNIATFIVGHVTKEGSLAGPRMLEHMVDTVLYFEGER
QHTFRILRAVKNRFGSTNEIGIFEMQSQGLVEVTNPSEIFLEERLEGATGSAIVVSMEGTRPILVEIQALVTPTIFGNAR
RTTSGLDFNRVSLIMAVLEKRANLLLQNQDAYLKSAGGVKLDEPAIDLAIAVAIASSYKEKPTAPDVAFIGEIGLTGEIR
RVNRIESRINEAAKLGFKKLYAPKNSLAGLEIPPSIEVIGVTTLAEVLQKVFA

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=949009 WDW95_RS09530 WP_368651520.1 1922856..1924217(-) (radA) [Lactococcus raffinolactis strain APC3967]
ATGGCGAAAGCAAAATCAAAATTCGTCTGCCAATCCTGTGGGTATCAGTCGCCCAAATATCTGGGGCGCTGTCCTAATTG
TGGCGCTTGGAACTCTTTTGTCGAAGAAATTGAAGCCAAAGAGGTCAAAAATGCGCGCGTGACCCTGACTGGGGAGAAAA
CACGACCTGTTAAACTAGGAGAAGTCGAAAGTCTAGATACACCCCGTGTTTTGACAGATATGGGCGAGTTTAACCGCGTT
CTTGGCGGTGGCGTTGTACCGGGGAGCTTGATTCTTATCGGTGGTGATCCAGGGATTGGGAAGTCAACCATTCTCTTACA
AGTTTCTGTGCAGCTGGCAAATCTCGGGCGTGTCCTTTATGTGTCAGGCGAGGAATCAGCCCAACAAATCAAGATGCGAG
CTGAACGTTTGGGTGATTTGGATTCTGATTTCTACCTCTACACAGAAAATAATATGCAAAATATCCGAGCTGAAATTGAA
AAATTGCAGCCAGATTTTCTGGTCATTGACTCCATCCAAACCGTCATGAGCCCCGAAATTACAGGCGTACAAGGCTCGGT
CAGTCAAGTTCGAGAAGTGACCGCAGAACTACTCCAAATCGCAAAAACCAATAATATTGCTACCTTTATCGTCGGTCACG
TCACCAAGGAAGGCAGTCTTGCCGGCCCGAGAATGCTAGAGCATATGGTAGATACGGTGCTTTATTTTGAAGGGGAGCGC
CAGCATACTTTTCGGATTCTTCGTGCGGTGAAAAACCGGTTCGGCTCAACCAATGAAATCGGAATTTTTGAGATGCAATC
TCAAGGACTGGTCGAAGTGACCAATCCTAGCGAGATTTTCTTAGAAGAACGTTTAGAAGGCGCGACTGGCTCTGCTATTG
TTGTTTCAATGGAAGGGACCAGACCGATTTTGGTAGAAATTCAAGCGCTTGTCACACCAACGATTTTTGGTAATGCCAGA
CGAACAACTTCAGGTCTTGACTTTAATCGGGTCAGCTTGATCATGGCAGTGCTTGAAAAACGTGCCAATCTCCTGCTCCA
AAATCAAGATGCCTATCTCAAATCAGCTGGTGGTGTCAAATTAGATGAACCAGCCATTGATTTAGCCATTGCGGTTGCCA
TTGCTTCAAGTTATAAAGAAAAACCAACAGCACCAGATGTTGCCTTTATCGGTGAAATTGGCTTGACGGGCGAAATTCGC
CGGGTCAATCGCATCGAGAGTCGGATCAATGAGGCAGCTAAACTAGGCTTTAAAAAACTTTATGCCCCTAAAAATTCCTT
GGCTGGTTTAGAAATCCCACCATCTATAGAAGTCATTGGTGTCACGACTTTAGCCGAAGTCTTACAGAAGGTCTTTGCTT
AA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(82-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

81.457

100

0.815
  radA Streptococcus pneumoniae D39

81.457

100

0.815
  radA Streptococcus pneumoniae R6

81.457

100

0.815
  radA Streptococcus pneumoniae TIGR4

81.457

100

0.815
  radA Streptococcus mitis SK321

81.457

100

0.815
  radA Streptococcus mitis NCTC 12261

81.236

100

0.812
  radA Bacillus subtilis subsp. subtilis str. 168

64.68

100

0.647