Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   V8J88_RS19195 Genome accession   NZ_CP147776
Coordinates   4724219..4725706 (+) Length   495 a.a.
NCBI ID   WP_338845841.1    Uniprot ID   -
Organism   Massilia sp. W12     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4719219..4730706
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V8J88_RS19170 (V8J88_19170) - 4720726..4721745 (+) 1020 WP_338845835.1 KpsF/GutQ family sugar-phosphate isomerase -
  V8J88_RS19175 (V8J88_19175) - 4721745..4722287 (+) 543 WP_338845836.1 HAD hydrolase family protein -
  V8J88_RS19180 (V8J88_19180) lptC 4722295..4722900 (+) 606 WP_338845838.1 LPS export ABC transporter periplasmic protein LptC -
  V8J88_RS19185 (V8J88_19185) lptA 4722897..4723445 (+) 549 WP_338845839.1 lipopolysaccharide transport periplasmic protein LptA -
  V8J88_RS19190 (V8J88_19190) lptB 4723442..4724197 (+) 756 WP_338845840.1 LPS export ABC transporter ATP-binding protein -
  V8J88_RS19195 (V8J88_19195) rpoN/rpoN1 4724219..4725706 (+) 1488 WP_338845841.1 RNA polymerase factor sigma-54 Machinery gene
  V8J88_RS19200 (V8J88_19200) raiA 4725871..4726236 (+) 366 WP_338845842.1 ribosome-associated translation inhibitor RaiA -
  V8J88_RS19205 (V8J88_19205) - 4726652..4727737 (+) 1086 WP_338845843.1 enoyl-CoA hydratase/isomerase family protein -
  V8J88_RS19210 (V8J88_19210) - 4727745..4728905 (-) 1161 WP_338845844.1 deoxyguanosinetriphosphate triphosphohydrolase -
  V8J88_RS19215 (V8J88_19215) aroKB 4728902..4730581 (-) 1680 WP_338845845.1 bifunctional shikimate kinase/3-dehydroquinate synthase AroKB -

Sequence

Protein


Download         Length: 495 a.a.        Molecular weight: 54978.45 Da        Isoelectric Point: 5.0433

>NTDB_id=948544 V8J88_RS19195 WP_338845841.1 4724219..4725706(+) (rpoN/rpoN1) [Massilia sp. W12]
MKQSLQLRTSQHLALTPQLQQSIRLLQLSTLELQQELAQMLSENPMLERTDDPLDHSVRLLADGAISNSPASEAGMPSEN
PAPEAEAPATAEAGNSEENSFEHDGDGMLQDSLSFEDAPGARSADDEDMRPQLEDASNSLRDHLLEQMRVTARTQRDRAL
LEFLIDSLDENGYLEESLEALLESLPAELEIELEELQTSLKLLQSFDPPGVGARNLSECLALQIRQLPKAPMVTRRHALQ
IVEQHLPLLAARDYTRLKKAMDCDDEDLREAQALIRLCQPHPGAAFGASDKDHVAPDVIVKRNRNGWSVSLNPDVMPRLR
VNALYASMLKQNKGDGGLSNQLQEAKWLIKNMRQRYETILRVAQAIVERQRNFFAHGAVAMRPLVLREIADTLGLHESTI
SRVTTQKYMLTPHGMFELKYFFGSHVATEAGGEASSTAIRALIKQLIGAEDPKNPLSDSKLADMLGEQGMVVARRTVAKY
REALKIPAVALRKSL

Nucleotide


Download         Length: 1488 bp        

>NTDB_id=948544 V8J88_RS19195 WP_338845841.1 4724219..4725706(+) (rpoN/rpoN1) [Massilia sp. W12]
ATGAAACAGTCGCTGCAACTTCGCACCTCGCAACATCTGGCGCTGACGCCGCAATTGCAACAGTCGATCCGCCTGTTGCA
ACTCTCCACGCTTGAATTGCAGCAGGAATTGGCGCAGATGTTGTCGGAAAACCCCATGCTGGAGCGCACCGATGATCCGC
TCGATCATTCCGTGCGCTTGTTAGCCGACGGCGCCATCAGCAACAGCCCGGCGAGTGAGGCCGGCATGCCTTCAGAAAAT
CCGGCCCCCGAAGCGGAAGCGCCGGCAACTGCAGAAGCCGGCAACAGCGAAGAAAACAGCTTCGAGCATGATGGCGATGG
CATGCTGCAAGACAGCTTGAGTTTTGAAGATGCGCCGGGCGCGCGCTCGGCGGACGATGAAGATATGCGCCCGCAGCTGG
AAGACGCCAGCAACAGCCTGCGCGACCATTTGCTGGAACAAATGCGCGTCACTGCCCGCACCCAACGCGACCGCGCCCTG
CTTGAATTCCTGATAGACAGCTTAGATGAGAATGGCTATCTTGAAGAGAGCCTGGAAGCCTTGCTGGAAAGTCTGCCGGC
TGAATTGGAAATTGAATTGGAGGAATTGCAGACCAGTTTAAAACTGCTGCAAAGCTTTGACCCGCCCGGCGTTGGCGCGC
GCAATCTGTCTGAATGCCTGGCGCTGCAAATCCGCCAATTGCCAAAAGCGCCAATGGTGACGCGCCGCCATGCGCTGCAA
ATCGTCGAGCAGCATTTGCCGCTGCTGGCGGCGCGCGACTACACCAGGCTGAAAAAAGCCATGGACTGCGACGACGAGGA
TTTGCGCGAAGCGCAAGCCCTGATCCGTCTGTGCCAACCCCATCCGGGCGCCGCTTTTGGCGCTTCCGATAAAGACCATG
TGGCGCCGGATGTGATCGTCAAACGCAACCGCAACGGCTGGAGCGTGAGCTTGAATCCGGACGTGATGCCGCGCTTGCGC
GTGAATGCGCTGTACGCCTCGATGTTGAAGCAAAACAAGGGCGATGGCGGCTTGAGCAACCAACTGCAGGAGGCGAAATG
GCTGATCAAAAATATGCGTCAGCGCTATGAAACCATCCTCCGCGTGGCGCAAGCAATCGTGGAGCGGCAACGCAACTTTT
TCGCCCATGGCGCAGTTGCGATGCGCCCCCTTGTGTTACGTGAAATTGCTGATACACTAGGATTACACGAGAGCACAATC
TCGCGTGTGACGACTCAAAAATATATGCTGACCCCGCACGGCATGTTTGAGTTGAAATACTTCTTTGGCAGCCATGTCGC
CACTGAAGCGGGGGGCGAAGCGTCATCAACGGCGATACGCGCACTGATCAAGCAACTGATAGGAGCAGAAGATCCCAAAA
ATCCCCTTTCTGACAGCAAACTTGCAGATATGCTGGGTGAACAGGGTATGGTGGTCGCACGTCGCACTGTGGCCAAATAC
CGTGAAGCTCTGAAAATCCCGGCAGTGGCGCTGCGCAAAAGCCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(339-493)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(134-325)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

61.111

100

0.622