Detailed information NTG0948534

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: V8J88_RS03220
• Genome accession: NZ_CP147776
• Coordinates: 788127..789341 (-)
• Length: 404 a.a.
• NCBI ID: WP_338847740.1
• Uniprot ID: -
• Organism: Massilia sp. W12
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 783127..794341

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V8J88_RS03190 (V8J88_03190)	-	783504..784028 (+)	525	WP_338847734.1	GNAT family N-acetyltransferase	-
V8J88_RS03195 (V8J88_03195)	rpoH	784066..784965 (-)	900	WP_338847735.1	RNA polymerase sigma factor RpoH	-
V8J88_RS03200 (V8J88_03200)	-	785336..786025 (-)	690	WP_338847736.1	class I SAM-dependent methyltransferase	-
V8J88_RS03205 (V8J88_03205)	-	786012..786491 (-)	480	WP_338847737.1	hypothetical protein	-
V8J88_RS03210 (V8J88_03210)	-	786497..787423 (-)	927	WP_338847738.1	permease-like cell division protein FtsX	-
V8J88_RS03215 (V8J88_03215)	-	787420..788103 (-)	684	WP_338847739.1	ATP-binding cassette domain-containing protein	-
V8J88_RS03220 (V8J88_03220)	pilA	788127..789341 (-)	1215	WP_338847740.1	signal recognition particle-docking protein FtsY	Machinery gene
V8J88_RS03225 (V8J88_03225)	-	789622..790830 (-)	1209	WP_338847741.1	ABC transporter permease	-
V8J88_RS03230 (V8J88_03230)	-	790821..791573 (-)	753	WP_338847742.1	ABC transporter ATP-binding protein	-
V8J88_RS03235 (V8J88_03235)	-	791570..792820 (-)	1251	WP_338847743.1	efflux RND transporter periplasmic adaptor subunit	-
V8J88_RS03240 (V8J88_03240)	-	792947..794272 (-)	1326	WP_338847744.1	PepSY-associated TM helix domain-containing protein	-

Sequence

Protein

• Length: 404 a.a.
• Molecular weight: 42316.82 Da
• Isoelectric Point: 5.5051

>NTDB_id=948534 V8J88_RS03220 WP_338847740.1 788127..789341(-) (pilA) [Massilia sp. W12]
MFSFFKKKPADAAPAPALENQPTDTAATPPLTLPAETSQQTAPAAPLDAPAPALASALPVSAEVLSPPPAVLPLEQEQAF
APGIAYGDLSASSTETVAPPPNPAEGKRSWLARLKAGLAKTSANLSLLFLGAKIDDDLYEELEGALLMSDAGVEATQYLL
DALKKKVKDEKLTEAAQVKVALKTLLTELLQPLQKPFVLGRNQPLVMMIAGVNGAGKTTTIGKLAKHMQAHGQSVLLAAG
DTFRAAAREQLSIWGQRNNVTVIAQESGDPAAVAFDAVSAGRARKMDVVMVDTAGRLPTQLHLMEELKKIKRVLGKAQDG
APHEVLLVIDGNTGQNALAQVKAFDDALQLSGLVITKLDGTAKGGVLAAIARTRPVPVYFIGVGEKIEDLQAFNAAEFVD
ALLG

Nucleotide

• Length: 1215 bp

>NTDB_id=948534 V8J88_RS03220 WP_338847740.1 788127..789341(-) (pilA) [Massilia sp. W12]
ATGTTCAGTTTTTTCAAAAAAAAGCCGGCAGATGCCGCGCCAGCGCCTGCCCTGGAAAACCAGCCCACTGATACCGCCGC
CACGCCGCCGCTGACCTTGCCGGCAGAAACCTCGCAGCAAACTGCGCCAGCCGCCCCGCTTGATGCGCCGGCGCCGGCGC
TGGCATCCGCCCTGCCCGTCAGCGCAGAAGTTCTATCGCCGCCGCCCGCCGTGCTGCCGCTGGAGCAGGAACAAGCGTTT
GCGCCCGGCATCGCATATGGCGATTTATCCGCAAGCAGCACAGAAACCGTTGCGCCGCCGCCCAACCCGGCGGAAGGCAA
ACGCTCCTGGCTGGCGCGCTTGAAAGCCGGCCTGGCCAAAACCTCGGCCAATCTGTCCTTGCTCTTCCTCGGCGCCAAGA
TCGACGACGATTTATACGAAGAATTGGAAGGCGCGCTGTTGATGTCCGATGCCGGGGTCGAAGCCACCCAATATCTGCTG
GATGCGCTGAAAAAGAAGGTCAAGGATGAAAAATTGACCGAGGCCGCACAAGTCAAAGTCGCGCTGAAAACCCTGCTCAC
AGAATTGCTGCAACCGCTGCAAAAACCTTTCGTACTGGGGCGCAATCAACCCCTGGTGATGATGATCGCCGGCGTCAACG
GCGCTGGCAAAACCACCACCATCGGCAAGCTGGCCAAGCATATGCAAGCGCATGGCCAATCGGTGCTGCTGGCGGCTGGC
GACACCTTCCGCGCCGCCGCGCGTGAGCAACTCAGTATCTGGGGGCAGCGCAATAATGTCACCGTGATCGCGCAGGAATC
GGGCGACCCGGCGGCGGTGGCGTTTGACGCCGTCTCCGCAGGCCGCGCGCGCAAAATGGATGTGGTGATGGTCGATACCG
CTGGCCGCCTGCCAACCCAATTGCACTTGATGGAAGAGTTAAAAAAGATCAAGCGCGTATTGGGCAAGGCGCAGGATGGC
GCGCCGCATGAAGTTTTGCTGGTAATTGATGGCAATACCGGCCAGAACGCCTTGGCGCAAGTCAAAGCATTTGACGACGC
CTTGCAATTGAGCGGCCTGGTGATCACCAAACTCGACGGCACCGCCAAGGGCGGGGTATTGGCGGCGATTGCGCGCACCC
GTCCGGTGCCGGTGTATTTCATTGGCGTGGGTGAAAAGATTGAAGACTTGCAAGCGTTCAACGCCGCCGAATTCGTTGAC
GCCCTGCTTGGCTGA

Domains

Predicted by InterproScan.

• SRP54-type protein, helical bundle domain (SRP54_N) (131-184)

• SRP54-type protein, GTPase domain (SRP54) (205-404)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	51.415	100	0.54