Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   WDM00_RS01515 Genome accession   NZ_CP147726
Coordinates   318618..320333 (+) Length   571 a.a.
NCBI ID   WP_000788428.1    Uniprot ID   -
Organism   Vibrio cholerae strain CNCTC 6536     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 313618..325333
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WDM00_RS01495 (WDM00_01495) pilM 315911..316927 (+) 1017 WP_142737537.1 type IV pilus assembly protein PilM Machinery gene
  WDM00_RS01500 (WDM00_01500) pilN 316917..317501 (+) 585 WP_000902746.1 PilN domain-containing protein Machinery gene
  WDM00_RS01505 (WDM00_01505) pilO 317494..318084 (+) 591 WP_000157688.1 type 4a pilus biogenesis protein PilO Machinery gene
  WDM00_RS01510 (WDM00_01510) pilP 318074..318592 (+) 519 WP_000792032.1 pilus assembly protein PilP Machinery gene
  WDM00_RS01515 (WDM00_01515) pilQ 318618..320333 (+) 1716 WP_000788428.1 type IV pilus secretin PilQ Machinery gene
  WDM00_RS01520 (WDM00_01520) aroK 320520..321044 (+) 525 WP_000818616.1 shikimate kinase AroK -
  WDM00_RS01525 (WDM00_01525) aroB 321072..322157 (+) 1086 WP_000439804.1 3-dehydroquinate synthase -
  WDM00_RS01530 (WDM00_01530) - 322212..323687 (+) 1476 WP_367007818.1 AAA family ATPase -
  WDM00_RS01535 (WDM00_01535) - 323757..324590 (+) 834 WP_000744680.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62354.47 Da        Isoelectric Point: 4.5785

>NTDB_id=948222 WDM00_RS01515 WP_000788428.1 318618..320333(+) (pilQ) [Vibrio cholerae strain CNCTC 6536]
MKNGLKTYVAQTWLTLWVGLALCASSMVFSAESATANQLENIDFRVNKEKAAVLIVELASPSAVVDVQKVQEGLSIELLK
TDVADDKLYLLDVKDFSTPVESVEVFRKEPSTQLVVTVDGEFQHDYTLKGKYLEVVISKLKADEKPKPKSVLEKEGKLIS
INFQDIPVRNVLQLIADYNGFNLVVSDSVVGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILIAPKEELDLREKQAL
EKARLAEELGDLKSEIIKINFAKASDIAAMIGGEGNVNMLSERGSISIDERTNSLLIRELPDNIAVIREIIESLDIPVKQ
VQIEARIVTVKEGNLEELGVRWGVMSTNGSHSVGGTIESNLWQQGLLADDEFPVDEFLNVNLASTSANASSIAFQVAKLG
SGTLLDLELSALQNESKAEIISSPRLITTNKQPAYIEQGTEIPYLESSSSGASTVAFKKAVLSLKVTPQITPDNRLVLDL
SVTQDRRGETVKTGTGEAVSIDTQRIGTQVLVNNGETVVLGGIFQHSINNSVDKVPLLGDLPVLGALFRRTYEQMGKSEL
LIFVTPKVVIQ

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=948222 WDM00_RS01515 WP_000788428.1 318618..320333(+) (pilQ) [Vibrio cholerae strain CNCTC 6536]
ATGAAAAATGGATTGAAAACTTATGTCGCACAGACTTGGCTGACCTTGTGGGTTGGTTTGGCACTTTGTGCTTCTTCCAT
GGTGTTTAGTGCTGAATCCGCTACCGCCAACCAGTTGGAAAACATCGACTTTCGGGTCAACAAAGAGAAGGCGGCGGTCT
TAATTGTCGAATTGGCTTCGCCATCGGCGGTCGTGGATGTGCAAAAAGTCCAAGAAGGGCTGAGCATTGAACTCCTGAAA
ACCGATGTGGCGGATGACAAGCTGTATCTACTTGATGTGAAAGATTTTTCGACCCCTGTTGAGAGCGTGGAAGTATTCCG
TAAAGAGCCAAGCACACAACTTGTTGTGACTGTCGATGGGGAGTTTCAGCACGATTATACCTTGAAAGGAAAATACCTTG
AGGTCGTGATCAGTAAACTCAAAGCCGATGAAAAGCCTAAGCCCAAGAGTGTCTTGGAAAAAGAAGGCAAGCTGATTTCA
ATTAACTTCCAAGATATTCCGGTGCGTAACGTACTGCAGCTGATTGCCGATTATAACGGTTTCAACTTAGTGGTTTCCGA
TTCGGTGGTAGGTAACCTGACTTTACGCTTGGATGGCGTGCCTTGGCAGCAAGTGTTGGACATTATTCTGCAAGTCAAAG
GGCTCGATAAGCGGGTCGATGGCAATGTGATTTTGATTGCGCCGAAAGAGGAACTGGACTTGCGCGAGAAACAAGCGCTC
GAAAAAGCCCGTTTAGCAGAAGAGTTGGGCGATTTAAAATCAGAAATCATCAAGATTAACTTTGCCAAAGCGTCTGACAT
TGCGGCGATGATCGGCGGTGAAGGCAACGTGAACATGTTGTCTGAACGAGGTTCGATCAGCATCGACGAACGAACTAACT
CGCTATTGATCCGAGAGCTGCCCGATAATATTGCTGTGATCCGCGAGATCATTGAATCGCTGGATATTCCGGTCAAACAA
GTTCAAATCGAAGCGCGGATTGTCACCGTCAAAGAAGGTAACTTGGAGGAGCTGGGGGTACGCTGGGGCGTAATGTCGAC
CAATGGTAGTCACTCCGTGGGTGGAACCATTGAAAGTAACTTATGGCAGCAAGGTTTATTGGCAGATGATGAATTTCCGG
TGGATGAATTCTTAAATGTGAATTTGGCTTCCACCTCTGCCAATGCGTCTTCGATTGCTTTCCAAGTCGCTAAATTGGGC
TCAGGTACGTTGTTGGATTTGGAGCTTTCAGCATTACAAAATGAATCGAAGGCCGAAATCATCTCTAGCCCTCGTTTGAT
TACCACTAATAAACAGCCTGCCTATATTGAGCAAGGTACTGAGATCCCTTATCTGGAATCGTCTTCCAGTGGTGCCTCTA
CGGTCGCGTTTAAAAAAGCGGTATTAAGCTTGAAGGTGACACCACAAATCACCCCAGATAACCGCTTGGTGCTCGACTTG
AGTGTGACCCAAGACCGACGCGGTGAAACGGTGAAAACCGGTACGGGTGAAGCGGTATCGATCGATACTCAGCGTATTGG
CACTCAAGTGTTGGTGAATAACGGAGAAACTGTGGTGTTGGGTGGCATTTTCCAACACAGCATCAATAATTCGGTAGATA
AGGTGCCACTGCTGGGGGACTTGCCTGTTTTGGGGGCGTTATTCAGACGAACCTATGAGCAAATGGGCAAAAGTGAACTG
CTGATTTTTGTCACGCCGAAAGTGGTCATTCAATAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(411-569)

Bacterial type II/III secretion system short domain (Secretin_N)

(255-320)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae strain A1552

99.65

100

0.997
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

99.65

100

0.997
  pilQ Vibrio campbellii strain DS40M4

78.364

96.322

0.755
  pilQ Legionella pneumophila strain ERS1305867

42.963

94.571

0.406
  pilQ Legionella pneumophila strain Lp02

42.963

94.571

0.406
  comQ Acinetobacter baylyi ADP1

39.161

100

0.392
  pilQ Acinetobacter baumannii D1279779

37.819

100

0.389
  pilQ Acinetobacter baumannii strain A118

37.819

100

0.389
  pilQ Pseudomonas aeruginosa PAK

39.312

96.673

0.38