Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   WDM00_RS01495 Genome accession   NZ_CP147726
Coordinates   315911..316927 (+) Length   338 a.a.
NCBI ID   WP_142737537.1    Uniprot ID   -
Organism   Vibrio cholerae strain CNCTC 6536     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 310911..321927
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WDM00_RS01480 (WDM00_01480) - 311452..312183 (-) 732 WP_000495226.1 glutathione peroxidase -
  WDM00_RS01485 (WDM00_01485) oxyR 312323..313216 (+) 894 WP_001914100.1 DNA-binding transcriptional regulator OxyR -
  WDM00_RS01490 (WDM00_01490) - 313291..315768 (-) 2478 WP_185841317.1 penicillin-binding protein 1A -
  WDM00_RS01495 (WDM00_01495) pilM 315911..316927 (+) 1017 WP_142737537.1 type IV pilus assembly protein PilM Machinery gene
  WDM00_RS01500 (WDM00_01500) pilN 316917..317501 (+) 585 WP_000902746.1 PilN domain-containing protein Machinery gene
  WDM00_RS01505 (WDM00_01505) pilO 317494..318084 (+) 591 WP_000157688.1 type 4a pilus biogenesis protein PilO Machinery gene
  WDM00_RS01510 (WDM00_01510) pilP 318074..318592 (+) 519 WP_000792032.1 pilus assembly protein PilP Machinery gene
  WDM00_RS01515 (WDM00_01515) pilQ 318618..320333 (+) 1716 WP_000788428.1 type IV pilus secretin PilQ Machinery gene
  WDM00_RS01520 (WDM00_01520) aroK 320520..321044 (+) 525 WP_000818616.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37721.46 Da        Isoelectric Point: 7.3328

>NTDB_id=948218 WDM00_RS01495 WP_142737537.1 315911..316927(+) (pilM) [Vibrio cholerae strain CNCTC 6536]
MGKSLVTGIDIGHHSIKAAVLKPMGDTYALVGYEELLVTADIFTDNHTLDYQKIVKKLKELKKALPLFSHKVAIAIPDSA
VISKVLQIDSDLEQRELEFAVYQAFSHQSPFPVEELSLDFVKVTEKSLARSSTTTFQVYATKKDVVDSRLHAVKKAGFEP
VLMDVQVHSLLHLWQLASRAYRRPDWMLVDIGYTQSSLCLDFPEKMPFYKDVPLGTRQLESEPSAVNSLNHPLLHDPTQR
FIQELVDKIARQIQLFTSVHGTQSLGGLWLSGGGATLSGLEDALYQRLSLPCEVLNPFSLFKNNVAKRKRPLVDGQRFST
AAGLALRGLAWLESEHVA

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=948218 WDM00_RS01495 WP_142737537.1 315911..316927(+) (pilM) [Vibrio cholerae strain CNCTC 6536]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCTGCGGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGCTTTGCCTCTATTTAGTCATAAAGTCGCGATAGCAATCCCGGATAGCGCC
GTGATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCAGCGCGAGCTGGAATTTGCTGTCTATCAAGCCTTCTCGCA
TCAATCTCCATTCCCCGTTGAAGAGCTGAGCCTCGATTTCGTCAAAGTGACGGAAAAAAGCCTCGCCCGCAGCAGCACGA
CGACCTTCCAAGTGTATGCCACCAAAAAAGACGTGGTCGACAGTCGTTTGCATGCCGTTAAAAAAGCGGGTTTTGAACCT
GTGTTGATGGATGTGCAAGTACATAGTTTGTTGCATTTATGGCAACTCGCTAGCCGTGCGTATCGACGTCCAGACTGGAT
GTTGGTGGATATTGGCTACACCCAAAGTTCATTGTGCCTCGATTTTCCGGAAAAAATGCCCTTTTACAAAGACGTGCCAT
TAGGAACTCGTCAATTGGAATCAGAACCCAGTGCTGTGAACTCATTGAATCATCCACTGTTACACGATCCCACACAGCGG
TTTATTCAAGAATTGGTCGACAAAATTGCGCGGCAAATCCAGCTTTTTACTTCGGTGCACGGGACTCAAAGTCTTGGCGG
TTTATGGCTATCGGGTGGCGGTGCCACGCTTTCTGGTTTAGAGGACGCGCTTTATCAACGCTTGTCATTGCCTTGTGAAG
TGCTCAATCCGTTTTCACTGTTCAAAAACAATGTGGCAAAACGTAAACGCCCACTGGTTGATGGTCAGCGTTTTAGTACC
GCCGCCGGTCTTGCATTGCGAGGCTTAGCTTGGTTGGAGAGTGAGCATGTTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(241-328)

Type IV pilus assembly protein PilM; (PilM_2)

(8-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

99.704

100

0.997
  pilM Vibrio campbellii strain DS40M4

48.521

100

0.485