Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   IG620_RS02370 Genome accession   NZ_CP147682
Coordinates   511462..512262 (+) Length   266 a.a.
NCBI ID   WP_071850435.1    Uniprot ID   A0AAJ4P2Y2
Organism   Acinetobacter lwoffii strain CCTCC M20191004     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 506462..517262
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IG620_RS02340 (IG620_02340) - 506725..507135 (+) 411 WP_129716390.1 hypothetical protein -
  IG620_RS02345 (IG620_02345) - 507407..508687 (+) 1281 WP_338840698.1 glycosyltransferase family 1 protein -
  IG620_RS02350 (IG620_02350) - 508708..509259 (+) 552 WP_005107877.1 phosphatase PAP2 family protein -
  IG620_RS02355 (IG620_02355) iscX 509347..509547 (+) 201 WP_004278360.1 Fe-S cluster assembly protein IscX -
  IG620_RS02360 (IG620_02360) ndk 509658..510089 (+) 432 WP_004647460.1 nucleoside-diphosphate kinase -
  IG620_RS02365 (IG620_02365) rlmN 510212..511447 (+) 1236 WP_005107875.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  IG620_RS02370 (IG620_02370) pilF 511462..512262 (+) 801 WP_071850435.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  IG620_RS02375 (IG620_02375) - 512253..513044 (+) 792 WP_129716394.1 DUF4115 domain-containing protein -
  IG620_RS02380 (IG620_02380) ispG 513068..514189 (+) 1122 WP_129716395.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  IG620_RS02385 (IG620_02385) hisS 514193..515488 (+) 1296 WP_129716397.1 histidine--tRNA ligase -
  IG620_RS02390 (IG620_02390) - 515519..516217 (+) 699 WP_004278352.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30133.26 Da        Isoelectric Point: 9.2878

>NTDB_id=947998 IG620_RS02370 WP_071850435.1 511462..512262(+) (pilF) [Acinetobacter lwoffii strain CCTCC M20191004]
MRNLTSKFALISTVCVSLVLVACQTPDLGQKDPEKATKVRTQLAVEYLRTGDLDAAKRALDQALESSPRDSQANMMMGVL
LQQEGSKLNLEKADHYFKRAISADPKNAQARNNYGTYLYQLERYNDAIEQFQIAGATLGYDQRFRALENMGRIYLKLGDT
ANAEKSFKQALQANRDSYISMLELAEIFYLNQQFPAATQMYQQFVRGVGQKNQGARALWIGIRTARAGGDQLGMQVLVNQ
LRALFPESQEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=947998 IG620_RS02370 WP_071850435.1 511462..512262(+) (pilF) [Acinetobacter lwoffii strain CCTCC M20191004]
TTGAGAAATCTAACATCAAAGTTTGCTTTGATTTCAACAGTATGTGTGTCTTTGGTTTTAGTCGCGTGTCAGACGCCAGA
TCTGGGTCAGAAAGACCCTGAAAAGGCCACCAAAGTCCGTACCCAATTGGCGGTAGAATATCTGAGAACAGGTGACCTGG
ATGCAGCTAAACGTGCGCTGGATCAGGCACTTGAAAGCAGTCCGCGTGATTCTCAAGCGAATATGATGATGGGCGTGCTA
CTGCAACAGGAAGGTAGTAAGCTTAATCTCGAAAAAGCAGATCATTATTTCAAGCGTGCGATTTCGGCAGATCCGAAAAA
TGCGCAGGCGCGAAATAATTACGGCACTTATTTATATCAGCTTGAACGCTATAATGATGCGATCGAGCAGTTTCAAATTG
CTGGTGCCACGTTGGGTTATGATCAGCGATTCCGCGCACTGGAAAATATGGGGCGGATTTACCTGAAACTGGGTGATACA
GCAAATGCTGAAAAATCATTCAAACAGGCGCTACAAGCCAATCGCGATTCCTATATTTCAATGTTAGAGTTGGCAGAAAT
ATTTTATTTGAACCAGCAGTTCCCGGCAGCGACACAAATGTATCAACAGTTTGTGCGTGGTGTGGGGCAGAAAAATCAGG
GTGCCCGTGCACTCTGGATTGGCATTCGCACTGCGCGTGCCGGAGGTGATCAGTTGGGTATGCAGGTATTGGTCAACCAG
TTACGTGCACTTTTTCCTGAAAGCCAGGAATACCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(42-81)

Tetratricopeptide repeat (TPR_17)

(97-129)

Tetratricopeptide repeat (TPR_8)

(145-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

78.846

97.744

0.771
  pilF Acinetobacter baylyi ADP1

69.925

100

0.699