Detailed information NTG0947372

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: WCU44_RS02055
• Genome accession: NZ_CP147601
• Coordinates: 444087..445235 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain GN05219
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 439087..450235

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WCU44_RS02030 (WCU44_02030)	-	440238..440534 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
WCU44_RS02035 (WCU44_02035)	-	440531..441124 (-)	594	WP_003084544.1	YggT family protein	-
WCU44_RS02040 (WCU44_02040)	proC	441135..441956 (-)	822	WP_023081631.1	pyrroline-5-carboxylate reductase	-
WCU44_RS02045 (WCU44_02045)	-	441968..442660 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
WCU44_RS02050 (WCU44_02050)	pilT	442875..443909 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
WCU44_RS02055 (WCU44_02055)	pilU	444087..445235 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
WCU44_RS02060 (WCU44_02060)	czcD	445243..446142 (-)	900	WP_023081630.1	CDF family zinc transporter CzcD	-
WCU44_RS02065 (WCU44_02065)	-	446296..446700 (+)	405	WP_003084560.1	NINE protein	-
WCU44_RS02070 (WCU44_02070)	-	446943..448316 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
WCU44_RS02075 (WCU44_02075)	-	448313..449497 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=947372 WCU44_RS02055 WP_003100959.1 444087..445235(+) (pilU) [Pseudomonas aeruginosa strain GN05219]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=947372 WCU44_RS02055 WP_003100959.1 444087..445235(+) (pilU) [Pseudomonas aeruginosa strain GN05219]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382