Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACG4IU_RS11360 Genome accession   NZ_CP171664
Coordinates   2506127..2507590 (+) Length   487 a.a.
NCBI ID   WP_000804830.1    Uniprot ID   Q9KP49
Organism   Vibrio cholerae strain M813     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2501127..2512590
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACG4IU_RS11330 - 2501759..2502721 (+) 963 WP_000904330.1 calcium/sodium antiporter -
  ACG4IU_RS11335 kdsD 2502777..2503751 (+) 975 WP_001120614.1 arabinose-5-phosphate isomerase KdsD -
  ACG4IU_RS11340 kdsC 2503751..2504308 (+) 558 WP_000099768.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ACG4IU_RS11345 lptC 2504305..2504868 (+) 564 WP_000059348.1 LPS export ABC transporter periplasmic protein LptC -
  ACG4IU_RS11350 lptA 2504849..2505343 (+) 495 WP_000830444.1 lipopolysaccharide transport periplasmic protein LptA -
  ACG4IU_RS11355 lptB 2505346..2506071 (+) 726 WP_394861246.1 LPS export ABC transporter ATP-binding protein -
  ACG4IU_RS11360 rpoN/rpoN1 2506127..2507590 (+) 1464 WP_000804830.1 RNA polymerase factor sigma-54 Machinery gene
  ACG4IU_RS11365 hpf 2507615..2507902 (+) 288 WP_001166643.1 ribosome hibernation promoting factor -
  ACG4IU_RS11370 ptsN 2507905..2508351 (+) 447 WP_001177163.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACG4IU_RS11375 rapZ 2508364..2509224 (+) 861 WP_001237199.1 RNase adapter RapZ -
  ACG4IU_RS11380 - 2509233..2509511 (+) 279 WP_001135899.1 HPr family phosphocarrier protein -
  ACG4IU_RS11385 mgtE 2509672..2511033 (+) 1362 WP_394861248.1 magnesium transporter -
  ACG4IU_RS11390 pmbA 2511105..2512448 (-) 1344 WP_000382645.1 metalloprotease PmbA -

Sequence

Protein


Download         Length: 487 a.a.        Molecular weight: 55438.47 Da        Isoelectric Point: 4.2824

>NTDB_id=947154 ACG4IU_RS11360 WP_000804830.1 2506127..2507590(+) (rpoN/rpoN1) [Vibrio cholerae strain M813]
MKPSLQLKLGQQLAMTPQLQQAIRLLQLSTLDLQQEIQEALESNPLLEVEENQDEAPSLDSVRMTEESPREPEELYEPEP
QDSSDLIEKSEISAELEMDTTWDEVYSANTGSTGLALDDDAPIYQGETTQTLQDYLHWQLDLTPFSDVDRTIAVALIDAI
DDYGYLTVSLEEIQESLRSDDIELDEIEAVRKRIQQFDPFGVASLNLQDCLLLQLTTYPCDTPWLEEARLLLSQYIDDLG
NRDYKTILKETKLKEEDLREILQLIQQLDPRPGSRIAQDHAEYVIPDVSVYKEQGRWLVTINPDSVPKLKINQQYADLMR
GNNAESNYIRTNLQEAKWLIKSLESRNETLLKVAKCIVEHQHDFFEYGEEAMKPMVLNDVAMAVEMHESTISRVTTQKYM
HTPRGIFELKYFFSSHVSTDNGGECSSTAIRALIKKLVAAENPAKPLSDSKIATLLADQGIQVARRTIAKYRESLGIAPS
SQRKRLL

Nucleotide


Download         Length: 1464 bp        

>NTDB_id=947154 ACG4IU_RS11360 WP_000804830.1 2506127..2507590(+) (rpoN/rpoN1) [Vibrio cholerae strain M813]
ATGAAACCGTCATTACAGCTCAAGCTAGGTCAACAGTTAGCCATGACTCCGCAGCTCCAGCAGGCGATTCGTCTGTTGCA
GCTTTCGACGCTGGATCTGCAGCAAGAAATTCAGGAAGCATTGGAATCTAACCCCTTGTTAGAGGTGGAAGAGAACCAAG
ACGAAGCGCCATCGCTTGATAGCGTACGTATGACGGAAGAAAGCCCGCGCGAGCCAGAAGAACTCTACGAACCTGAACCG
CAGGATAGCTCGGATCTGATTGAAAAATCGGAAATCAGCGCTGAATTAGAGATGGATACCACTTGGGATGAAGTCTACAG
CGCCAATACTGGCAGTACAGGACTGGCACTCGATGATGATGCCCCTATCTACCAAGGCGAAACCACCCAAACCCTGCAAG
ATTATCTTCACTGGCAACTCGACTTAACTCCCTTTAGCGATGTGGATCGCACTATAGCCGTCGCACTTATCGATGCTATC
GACGATTACGGTTATTTAACCGTCTCACTAGAGGAGATCCAAGAGAGCCTGCGCAGTGATGACATTGAGTTGGATGAGAT
TGAAGCGGTACGTAAACGCATTCAGCAGTTTGACCCCTTTGGTGTGGCATCGCTTAATCTGCAAGACTGCTTGTTGCTGC
AACTCACCACCTATCCCTGTGATACTCCTTGGCTGGAAGAAGCGCGTTTACTGCTCTCACAGTACATCGATGATTTAGGG
AATCGCGATTACAAAACCATTCTGAAAGAGACTAAGCTCAAAGAAGAGGACTTGCGCGAGATCCTGCAACTCATCCAACA
GCTCGATCCTCGCCCCGGCAGTCGAATTGCCCAAGATCACGCTGAATACGTCATACCTGACGTGTCAGTCTATAAAGAAC
AAGGTCGATGGCTGGTCACCATCAACCCAGATAGTGTGCCTAAACTGAAGATCAATCAGCAGTATGCCGACCTGATGCGC
GGTAATAATGCGGAGAGCAACTACATCCGTACCAATTTGCAAGAGGCAAAATGGCTGATCAAAAGTTTAGAAAGTAGAAA
CGAGACACTGCTCAAAGTTGCCAAGTGCATAGTTGAACACCAACACGATTTCTTCGAGTATGGAGAAGAGGCGATGAAGC
CGATGGTGCTCAACGATGTGGCGATGGCGGTGGAAATGCATGAATCGACAATTTCGCGTGTCACCACGCAGAAATACATG
CATACCCCGCGCGGCATTTTTGAGTTGAAGTACTTTTTCTCCAGCCACGTCAGTACTGACAATGGCGGAGAGTGCTCGTC
CACTGCGATCAGGGCGCTGATCAAAAAACTGGTGGCGGCAGAGAATCCTGCCAAACCACTCAGTGACAGCAAGATCGCAA
CCCTACTCGCTGACCAAGGCATCCAAGTCGCGCGGCGAACCATCGCCAAATACCGTGAATCACTTGGCATCGCCCCATCA
AGTCAGCGCAAACGCCTGCTATAG

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-49)

Sigma-54, DNA binding domain (Sigma54_DBD)

(327-485)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(128-315)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9KP49

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.533

100

0.46