Detailed information NTG0947030

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: WCU30_RS03015
• Genome accession: NZ_CP147557
• Coordinates: 629554..630702 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain GN04821
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 624554..635702

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WCU30_RS02990 (WCU30_02990)	-	625705..626001 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
WCU30_RS02995 (WCU30_02995)	-	625998..626591 (-)	594	WP_003084544.1	YggT family protein	-
WCU30_RS03000 (WCU30_03000)	proC	626602..627423 (-)	822	WP_003142635.1	pyrroline-5-carboxylate reductase	-
WCU30_RS03005 (WCU30_03005)	-	627435..628127 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
WCU30_RS03010 (WCU30_03010)	pilT	628342..629376 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
WCU30_RS03015 (WCU30_03015)	pilU	629554..630702 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
WCU30_RS03020 (WCU30_03020)	czcD	630710..631609 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
WCU30_RS03025 (WCU30_03025)	-	631764..632168 (+)	405	WP_003084560.1	NINE protein	-
WCU30_RS03030 (WCU30_03030)	-	632411..633784 (+)	1374	WP_003459266.1	pyridoxal-phosphate dependent enzyme	-
WCU30_RS03035 (WCU30_03035)	-	633781..634965 (+)	1185	WP_016852373.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=947030 WCU30_RS03015 WP_003100959.1 629554..630702(+) (pilU) [Pseudomonas aeruginosa strain GN04821]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=947030 WCU30_RS03015 WP_003100959.1 629554..630702(+) (pilU) [Pseudomonas aeruginosa strain GN04821]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382