Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACG4I9_RS01800 Genome accession   NZ_CP171662
Coordinates   369020..370483 (-) Length   487 a.a.
NCBI ID   WP_000804830.1    Uniprot ID   Q9KP49
Organism   Vibrio cholerae strain M814     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 364020..375483
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACG4I9_RS01770 pmbA 364162..365505 (+) 1344 WP_000382645.1 metalloprotease PmbA -
  ACG4I9_RS01775 mgtE 365577..366938 (-) 1362 WP_000840452.1 magnesium transporter -
  ACG4I9_RS01780 - 367099..367377 (-) 279 WP_001135899.1 HPr family phosphocarrier protein -
  ACG4I9_RS01785 rapZ 367386..368246 (-) 861 WP_001237199.1 RNase adapter RapZ -
  ACG4I9_RS01790 ptsN 368259..368705 (-) 447 WP_001177163.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACG4I9_RS01795 hpf 368708..368995 (-) 288 WP_001166643.1 ribosome hibernation promoting factor -
  ACG4I9_RS01800 rpoN/rpoN1 369020..370483 (-) 1464 WP_000804830.1 RNA polymerase factor sigma-54 Machinery gene
  ACG4I9_RS01805 lptB 370539..371264 (-) 726 WP_000996178.1 LPS export ABC transporter ATP-binding protein -
  ACG4I9_RS01810 lptA 371267..371761 (-) 495 WP_000830444.1 lipopolysaccharide transport periplasmic protein LptA -
  ACG4I9_RS01815 lptC 371742..372305 (-) 564 WP_000059348.1 LPS export ABC transporter periplasmic protein LptC -
  ACG4I9_RS01820 kdsC 372302..372859 (-) 558 WP_000099768.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ACG4I9_RS01825 kdsD 372859..373833 (-) 975 WP_001120614.1 arabinose-5-phosphate isomerase KdsD -
  ACG4I9_RS01830 - 373889..374851 (-) 963 WP_000904330.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 487 a.a.        Molecular weight: 55438.47 Da        Isoelectric Point: 4.2824

>NTDB_id=947024 ACG4I9_RS01800 WP_000804830.1 369020..370483(-) (rpoN/rpoN1) [Vibrio cholerae strain M814]
MKPSLQLKLGQQLAMTPQLQQAIRLLQLSTLDLQQEIQEALESNPLLEVEENQDEAPSLDSVRMTEESPREPEELYEPEP
QDSSDLIEKSEISAELEMDTTWDEVYSANTGSTGLALDDDAPIYQGETTQTLQDYLHWQLDLTPFSDVDRTIAVALIDAI
DDYGYLTVSLEEIQESLRSDDIELDEIEAVRKRIQQFDPFGVASLNLQDCLLLQLTTYPCDTPWLEEARLLLSQYIDDLG
NRDYKTILKETKLKEEDLREILQLIQQLDPRPGSRIAQDHAEYVIPDVSVYKEQGRWLVTINPDSVPKLKINQQYADLMR
GNNAESNYIRTNLQEAKWLIKSLESRNETLLKVAKCIVEHQHDFFEYGEEAMKPMVLNDVAMAVEMHESTISRVTTQKYM
HTPRGIFELKYFFSSHVSTDNGGECSSTAIRALIKKLVAAENPAKPLSDSKIATLLADQGIQVARRTIAKYRESLGIAPS
SQRKRLL

Nucleotide


Download         Length: 1464 bp        

>NTDB_id=947024 ACG4I9_RS01800 WP_000804830.1 369020..370483(-) (rpoN/rpoN1) [Vibrio cholerae strain M814]
ATGAAACCGTCATTACAGCTCAAGCTAGGTCAACAGTTAGCCATGACTCCGCAGCTCCAGCAGGCGATTCGTCTGTTGCA
GCTTTCGACGCTGGATCTGCAGCAAGAAATTCAGGAAGCATTGGAATCTAACCCCTTGTTAGAGGTGGAAGAGAACCAAG
ACGAAGCGCCATCGCTTGATAGCGTACGTATGACGGAAGAAAGCCCGCGCGAGCCAGAAGAACTCTACGAACCTGAACCG
CAGGATAGCTCGGATCTGATTGAAAAATCGGAAATCAGCGCTGAATTAGAGATGGATACCACTTGGGATGAAGTCTACAG
CGCCAATACTGGCAGTACAGGACTGGCACTCGATGATGATGCCCCTATCTACCAAGGCGAAACCACCCAAACCCTGCAAG
ATTATCTTCACTGGCAACTCGACTTAACTCCCTTTAGCGATGTGGATCGCACTATAGCCGTCGCACTTATCGATGCTATC
GACGATTACGGTTATTTAACCGTCTCACTAGAGGAGATCCAAGAGAGCCTGCGCAGTGATGACATTGAGTTGGATGAGAT
TGAAGCGGTACGTAAACGCATTCAGCAGTTTGACCCCTTTGGTGTGGCATCGCTTAATCTGCAAGACTGCTTGTTGCTGC
AACTCACCACCTATCCCTGTGATACTCCTTGGCTGGAAGAAGCGCGTTTACTGCTCTCACAGTACATCGATGATTTAGGG
AATCGCGATTACAAAACCATTCTGAAAGAGACTAAGCTCAAAGAAGAGGACTTGCGCGAGATCCTGCAACTCATCCAACA
GCTCGATCCTCGCCCCGGCAGTCGAATTGCCCAAGATCACGCTGAATACGTCATACCTGACGTGTCAGTCTATAAAGAAC
AAGGTCGATGGCTGGTCACCATCAACCCAGATAGTGTGCCTAAACTGAAGATCAATCAGCAGTATGCCGACCTGATGCGC
GGTAATAATGCGGAGAGCAACTACATCCGTACCAATTTGCAAGAGGCAAAATGGCTGATCAAAAGTTTAGAAAGTAGAAA
CGAGACACTGCTCAAAGTTGCCAAGTGCATAGTTGAACACCAACACGATTTCTTCGAGTATGGAGAAGAGGCGATGAAGC
CGATGGTGCTCAACGATGTGGCGATGGCGGTGGAAATGCATGAATCGACAATTTCGCGTGTCACCACGCAGAAATACATG
CATACCCCGCGCGGCATTTTTGAGTTGAAGTACTTTTTCTCCAGCCACGTCAGTACTGACAATGGCGGAGAGTGCTCGTC
CACTGCGATCAGGGCGCTGATCAAAAAACTGGTGGCGGCAGAGAATCCTGCCAAACCACTCAGTGACAGCAAGATCGCAA
CCCTACTCGCTGACCAAGGCATCCAAGTCGCGCGGCGAACCATCGCCAAATACCGTGAATCACTTGGCATCGCCCCATCA
AGTCAGCGCAAACGCCTGCTATAG

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-49)

Sigma-54, DNA binding domain (Sigma54_DBD)

(327-485)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(128-315)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9KP49

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.533

100

0.46