Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DBT37_RS00345 Genome accession   NZ_CP147497
Coordinates   75574..76962 (+) Length   462 a.a.
NCBI ID   WP_111862492.1    Uniprot ID   -
Organism   Aerococcus mictus strain UMB0267     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 70574..81962
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DBT37_RS00330 (DBT37_0000330) - 71435..74362 (+) 2928 WP_146745167.1 insulinase family protein -
  DBT37_RS00335 (DBT37_0000335) - 74691..74876 (-) 186 WP_111862493.1 hypothetical protein -
  DBT37_RS00340 (DBT37_0000340) - 75045..75554 (+) 510 WP_111862507.1 dUTP diphosphatase -
  DBT37_RS00345 (DBT37_0000345) radA 75574..76962 (+) 1389 WP_111862492.1 DNA repair protein RadA Machinery gene
  DBT37_RS00350 (DBT37_0000350) - 77049..78221 (+) 1173 WP_070559672.1 PIN/TRAM domain-containing protein -
  DBT37_RS00355 (DBT37_0000355) - 78334..79149 (-) 816 WP_111862491.1 HAD family hydrolase -
  DBT37_RS00360 (DBT37_0000360) - 79316..80017 (+) 702 WP_111862490.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 462 a.a.        Molecular weight: 50372.61 Da        Isoelectric Point: 6.6161

>NTDB_id=946487 DBT37_RS00345 WP_111862492.1 75574..76962(+) (radA) [Aerococcus mictus strain UMB0267]
MAKKKRKTVYTCQACGYESPQWYGKCPQCGAWNTLEEEVIAGKPIRQAGTEVNQKKAKPERLKEVSQAEEKRVKTGLGEF
NRVLGGGVVPGSLVLIGGDPGIGKSTLLLQVAKQLSDNGGRVLYVSGEESLHQIKMRADRLGYQDADFFVYAETDLLAIQ
AAISDTQADFVVIDSIQTMVHPNNESLAGSVSQVREATGELMHIAKSSGIAIFVVGHVTKEGNIAGPRILEHMVDTVLYL
EGEKHNTFRILRAVKNRFGSTNEIGVFDMKSQGLEEVTNPSQLFLEERLMGANGSAVVASMEGTRPILTEIQALLSATAF
GNARRTASGLDYSRVTLIMAVLEKRAHLMLQNQDAYLKSTGGVKLDEPAIDLAIAVAVASSYWEKETQASDCFVGEIGLT
GEIRRISRINERIQEAEKLGFKRIFIPYGNLQGLATDSQDIEIIGAKTLTQVLKEVFPPQGK

Nucleotide


Download         Length: 1389 bp        

>NTDB_id=946487 DBT37_RS00345 WP_111862492.1 75574..76962(+) (radA) [Aerococcus mictus strain UMB0267]
ATGGCTAAAAAGAAGCGAAAAACTGTCTACACTTGCCAGGCCTGTGGCTATGAATCGCCCCAGTGGTATGGGAAATGTCC
CCAATGTGGGGCTTGGAATACCCTGGAAGAGGAAGTGATTGCGGGTAAGCCCATCCGCCAAGCTGGGACAGAGGTCAATC
AGAAAAAAGCCAAGCCTGAACGCCTCAAGGAAGTCAGCCAGGCGGAAGAAAAACGGGTAAAGACTGGTTTAGGCGAGTTC
AACCGGGTCCTCGGTGGCGGGGTAGTTCCTGGATCTTTGGTCCTGATTGGTGGAGACCCCGGCATTGGGAAGTCGACCCT
GCTCCTCCAGGTAGCTAAGCAATTAAGTGATAATGGTGGCCGGGTCCTCTATGTTTCCGGTGAAGAAAGTTTACATCAAA
TCAAGATGCGGGCTGACCGCCTAGGCTACCAGGATGCGGACTTTTTTGTTTATGCCGAAACCGACCTTTTGGCCATTCAA
GCGGCGATTTCTGATACCCAGGCCGACTTTGTGGTGATTGACTCCATCCAAACCATGGTCCATCCCAATAATGAATCCCT
GGCCGGCAGTGTCAGTCAGGTCAGAGAAGCGACCGGAGAACTTATGCATATTGCTAAGAGCTCTGGGATCGCCATCTTTG
TGGTCGGCCATGTGACCAAGGAAGGCAATATTGCTGGGCCGCGGATTCTTGAACACATGGTGGACACGGTCTTGTACTTG
GAAGGGGAGAAGCATAACACCTTCCGGATCCTCAGAGCGGTCAAAAACCGTTTTGGCTCGACCAATGAAATCGGAGTCTT
CGATATGAAAAGCCAGGGCCTGGAAGAGGTCACCAACCCTAGCCAGCTCTTTTTAGAAGAACGACTCATGGGAGCCAATG
GGTCGGCAGTGGTTGCTTCCATGGAAGGAACCCGTCCAATCCTAACCGAAATCCAAGCCCTCTTGTCGGCTACGGCCTTT
GGAAATGCCCGCCGTACGGCCAGTGGTTTGGACTATTCCAGGGTGACTCTCATTATGGCGGTCTTAGAGAAGCGGGCCCA
TCTCATGCTGCAAAACCAAGATGCTTATCTCAAGTCAACCGGTGGGGTTAAACTCGATGAACCCGCCATTGACCTGGCCA
TTGCCGTAGCCGTGGCTTCCAGTTACTGGGAGAAGGAGACCCAGGCTAGCGATTGCTTTGTGGGTGAAATTGGCCTGACC
GGTGAAATTCGCCGGATTTCCCGGATTAATGAACGGATCCAAGAAGCGGAAAAATTAGGCTTTAAACGGATTTTTATCCC
CTATGGAAACCTGCAAGGCCTGGCGACCGATAGTCAAGATATTGAAATTATTGGCGCCAAGACCCTGACCCAAGTTCTCA
AAGAAGTCTTTCCCCCTCAAGGAAAATAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(367-430)

AAA domain (AAA_25)

(68-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(10-37)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

63.355

98.052

0.621
  radA Streptococcus pneumoniae Rx1

63.355

98.052

0.621
  radA Streptococcus pneumoniae D39

63.355

98.052

0.621
  radA Streptococcus pneumoniae R6

63.355

98.052

0.621
  radA Streptococcus pneumoniae TIGR4

63.355

98.052

0.621
  radA Streptococcus mitis NCTC 12261

63.135

98.052

0.619
  radA Bacillus subtilis subsp. subtilis str. 168

62.719

98.701

0.619