Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   WBS32_RS02245 Genome accession   NZ_CP147495
Coordinates   403669..405462 (+) Length   597 a.a.
NCBI ID   WP_338754104.1    Uniprot ID   -
Organism   Leuconostoc mesenteroides strain Lm10     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 398669..410462
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WBS32_RS02225 (WBS32_02225) - 398844..400286 (+) 1443 WP_011679287.1 ABC transporter substrate-binding protein/permease -
  WBS32_RS02230 (WBS32_02230) - 400283..401035 (+) 753 WP_011679288.1 ATP-binding cassette domain-containing protein -
  WBS32_RS02235 (WBS32_02235) - 401102..402547 (+) 1446 WP_144902429.1 ABC transporter substrate-binding protein/permease -
  WBS32_RS02240 (WBS32_02240) - 402589..403611 (+) 1023 WP_014324325.1 competence protein CoiA family protein -
  WBS32_RS02245 (WBS32_02245) pepF 403669..405462 (+) 1794 WP_338754104.1 oligoendopeptidase F Regulator
  WBS32_RS02250 (WBS32_02250) - 405567..405962 (+) 396 WP_338754106.1 cytidine deaminase -
  WBS32_RS02255 (WBS32_02255) - 405979..406419 (+) 441 WP_004164656.1 nucleoside 2-deoxyribosyltransferase -
  WBS32_RS02260 (WBS32_02260) - 406419..407348 (+) 930 WP_011679292.1 phosphoglycerate dehydrogenase -
  WBS32_RS02265 (WBS32_02265) - 407368..407736 (+) 369 WP_338754109.1 winged helix-turn-helix transcriptional regulator -
  WBS32_RS02270 (WBS32_02270) pheS 408040..409089 (+) 1050 WP_025267790.1 phenylalanine--tRNA ligase subunit alpha -

Sequence

Protein


Download         Length: 597 a.a.        Molecular weight: 67790.54 Da        Isoelectric Point: 4.6399

>NTDB_id=946468 WBS32_RS02245 WP_338754104.1 403669..405462(+) (pepF) [Leuconostoc mesenteroides strain Lm10]
MSEKQLLRNQVPENLTWDLSSIFPTDEAWETAFNETRKSAQALSKYAGHVGDSADVLKEALEADLAVERQFEKVYVYAHQ
IYDQDTTNQKYSAYNARVQGLYAELSANTAFFQPEVLVLSNETLDAYLATAGLKKYAHFFDVLKARKPHTLPAEQEALLA
GASDIFNASEQTFGALDNSDIVFGDVHTETGEIVPLTNGLYSLLLESKDRSLREEAFTTLYDSYIALQNTFASTLSSHVK
GHNFLAKTRHYDSARQAAVTANDIPESVFETLTKTVDANLPLLHRFVSLRKKILNVDDVHSYDLYVPLVDEINFEVTYEK
AKDIVLAALAPLGEDYIAIVQKAFDERWIDVVENKGKRTGAYSSGSYDTKPFILLNWQNNLNNVYTLAHEMGHSVHSYFT
RHNQEYHYGDYPIFLAEIASTTNEGLLTDYLLKTNDDPKFQAYVLNQYLDGFKGTVFRQTQFAEFEQWIHEQSAAGVALT
ADEMSTFYGSLNQKFYGSDLFPDEAIAYEWARIPHFYYNFYVYQYSTGEAAATTLAERILTQNGAEDYKNYLKAGNSDYA
LNVIKKAGVNMSQPDYLNEAFKVFEERLNEFEKIMLK

Nucleotide


Download         Length: 1794 bp        

>NTDB_id=946468 WBS32_RS02245 WP_338754104.1 403669..405462(+) (pepF) [Leuconostoc mesenteroides strain Lm10]
ATGTCTGAAAAACAACTATTACGTAATCAAGTACCAGAAAATCTTACATGGGATCTATCCTCAATTTTTCCGACAGATGA
AGCTTGGGAAACCGCTTTTAATGAGACACGAAAGTCTGCACAAGCATTAAGTAAGTACGCTGGACACGTCGGGGATTCTG
CGGATGTTTTGAAAGAAGCCTTAGAAGCAGATTTAGCAGTAGAACGTCAATTCGAAAAAGTCTACGTATATGCTCATCAA
ATTTATGATCAAGATACAACTAATCAAAAGTATAGTGCGTATAATGCTCGTGTTCAAGGCTTATATGCTGAATTAAGTGC
GAATACAGCCTTCTTCCAGCCTGAAGTGTTAGTATTGTCAAACGAGACACTAGATGCATACTTAGCGACAGCTGGTTTGA
AAAAATATGCACACTTTTTTGACGTTTTAAAAGCTCGTAAACCGCATACGCTACCTGCAGAACAGGAAGCCTTACTAGCA
GGAGCAAGCGACATCTTTAATGCTTCTGAACAGACTTTTGGTGCATTGGATAATTCAGATATTGTTTTTGGTGATGTCCA
TACTGAAACAGGTGAAATTGTGCCATTGACAAATGGACTCTATTCATTGTTGTTGGAGTCTAAAGATCGTTCATTACGTG
AGGAAGCGTTCACAACGCTTTATGATAGTTATATTGCTTTGCAAAATACATTTGCCTCAACTTTATCTTCACATGTCAAG
GGACATAACTTTTTGGCTAAAACCCGTCACTATGACTCAGCTCGTCAAGCTGCTGTAACAGCCAATGATATTCCTGAGTC
AGTTTTTGAAACATTAACCAAAACAGTTGATGCCAATCTGCCATTACTACACCGTTTTGTATCATTACGTAAAAAAATTC
TTAATGTTGACGATGTACACTCTTACGATTTATATGTACCACTTGTTGATGAAATTAATTTTGAAGTAACATATGAAAAG
GCAAAAGACATTGTTTTGGCGGCTTTGGCACCGTTAGGCGAGGATTACATCGCTATTGTTCAAAAGGCTTTTGATGAACG
ATGGATTGATGTGGTTGAAAATAAGGGGAAGCGTACTGGCGCTTATTCAAGCGGTTCGTATGATACAAAACCATTCATTT
TGCTTAACTGGCAGAACAATTTGAATAATGTTTATACGTTGGCTCATGAAATGGGACATTCGGTTCATTCATACTTTACA
CGCCATAATCAAGAGTATCATTATGGTGATTATCCAATATTTTTAGCTGAAATTGCTTCAACTACTAATGAGGGATTATT
AACGGATTATTTGCTAAAGACAAATGATGATCCCAAGTTTCAGGCCTACGTATTGAACCAGTATCTAGATGGATTCAAAG
GAACAGTTTTCCGCCAAACTCAATTTGCTGAGTTTGAGCAATGGATTCATGAACAAAGTGCGGCTGGAGTTGCTTTGACA
GCTGATGAGATGAGTACATTCTATGGATCGTTGAATCAAAAGTTTTATGGATCGGACTTATTTCCAGATGAAGCTATTGC
TTATGAATGGGCACGTATTCCACATTTTTATTACAATTTCTATGTTTATCAATATTCGACAGGCGAAGCTGCTGCCACAA
CTTTGGCAGAACGCATACTAACACAAAATGGTGCTGAGGATTATAAGAACTATCTGAAAGCAGGTAATTCAGATTATGCT
TTAAATGTGATCAAAAAAGCTGGCGTTAACATGAGTCAACCGGACTATTTAAATGAAGCTTTCAAAGTGTTTGAAGAGCG
ATTAAATGAGTTTGAAAAAATCATGTTAAAGTAA

Domains

Predicted by InterproScan.

Peptidase family M3 (Peptidase_M3)

(204-582)

Oligopeptidase F (Peptidase_M3_N)

(115-183)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

52.277

99.33

0.519