Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   WBM82_RS13765 Genome accession   NZ_CP147494
Coordinates   2591096..2591506 (-) Length   136 a.a.
NCBI ID   WP_160221688.1    Uniprot ID   -
Organism   Bacillus subtilis strain YT1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2586096..2596506
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WBM82_RS13735 (WBM82_13780) - 2586183..2586323 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  WBM82_RS13740 (WBM82_13785) yqeF 2586629..2587360 (-) 732 WP_029317882.1 SGNH/GDSL hydrolase family protein -
  WBM82_RS13745 (WBM82_13790) cwlH 2587612..2588364 (-) 753 WP_160221685.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  WBM82_RS13750 (WBM82_13795) yqeD 2588551..2589177 (+) 627 WP_160221686.1 TVP38/TMEM64 family protein -
  WBM82_RS13755 (WBM82_13800) gnd 2589196..2590089 (-) 894 WP_043857850.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  WBM82_RS13760 (WBM82_13805) yqeB 2590341..2591063 (+) 723 WP_160221687.1 DUF308 domain-containing protein -
  WBM82_RS13765 (WBM82_13810) nucA/comI 2591096..2591506 (-) 411 WP_160221688.1 sporulation-specific Dnase NucB Machinery gene
  WBM82_RS13770 (WBM82_13815) - 2591698..2592045 (+) 348 Protein_2567 sigma-70 family RNA polymerase sigma factor -
  WBM82_RS13775 (WBM82_13820) spoIVCA 2592076..2593536 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  WBM82_RS13780 (WBM82_13825) - 2593494..2593672 (-) 179 Protein_2569 hypothetical protein -
  WBM82_RS13785 (WBM82_13830) arsC 2594027..2594446 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  WBM82_RS13790 (WBM82_13835) acr3 2594458..2595498 (-) 1041 WP_004398718.1 arsenite efflux transporter Acr3 -
  WBM82_RS13795 (WBM82_13840) yqcK 2595521..2595961 (-) 441 WP_003229954.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  WBM82_RS13800 (WBM82_13845) arsR 2596022..2596339 (-) 318 WP_004399122.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14994.06 Da        Isoelectric Point: 5.1853

>NTDB_id=946436 WBM82_RS13765 WP_160221688.1 2591096..2591506(-) (nucA/comI) [Bacillus subtilis strain YT1]
MKKWMAGLFLAAAVLLCLMVPQQIQGASLYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=946436 WBM82_RS13765 WP_160221688.1 2591096..2591506(-) (nucA/comI) [Bacillus subtilis strain YT1]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTTGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTACCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529