Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   WBI56_RS12985 Genome accession   NZ_CP147492
Coordinates   2516744..2517154 (-) Length   136 a.a.
NCBI ID   WP_338730599.1    Uniprot ID   -
Organism   Bacillus subtilis strain MC4-2     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2511744..2522154
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WBI56_RS12955 (WBI56_12955) - 2511829..2511969 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  WBI56_RS12960 (WBI56_12960) yqeF 2512275..2513006 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  WBI56_RS12965 (WBI56_12965) cwlH 2513258..2514010 (-) 753 WP_187953713.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  WBI56_RS12970 (WBI56_12970) yqeD 2514197..2514823 (+) 627 WP_338730596.1 TVP38/TMEM64 family protein -
  WBI56_RS12975 (WBI56_12975) gnd 2514842..2515735 (-) 894 WP_338730597.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  WBI56_RS12980 (WBI56_12980) yqeB 2515989..2516711 (+) 723 WP_338730598.1 DUF308 domain-containing protein -
  WBI56_RS12985 (WBI56_12985) nucA/comI 2516744..2517154 (-) 411 WP_338730599.1 sporulation-specific Dnase NucB Machinery gene
  WBI56_RS12990 (WBI56_12990) - 2517350..2517697 (+) 348 Protein_2509 sigma-70 family RNA polymerase sigma factor -
  WBI56_RS12995 (WBI56_12995) spoIVCA 2517728..2519188 (-) 1461 WP_223203936.1 site-specific DNA recombinase SpoIVCA -
  WBI56_RS13000 (WBI56_13000) - 2519146..2519324 (-) 179 Protein_2511 hypothetical protein -
  WBI56_RS13005 (WBI56_13005) - 2519981..2520574 (-) 594 WP_080528950.1 TetR/AcrR family transcriptional regulator -
  WBI56_RS13010 (WBI56_13010) - 2520733..2521728 (+) 996 WP_088299191.1 acryloyl-CoA reductase -
  WBI56_RS13015 (WBI56_13015) - 2522010..2522140 (+) 131 Protein_2514 DUF255 domain-containing protein -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 15039.05 Da        Isoelectric Point: 5.1853

>NTDB_id=946357 WBI56_RS12985 WP_338730599.1 2516744..2517154(-) (nucA/comI) [Bacillus subtilis strain MC4-2]
MKKWMAGLFLSAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGVGADVRYVTPSDNRGAGSWVGNQMNSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=946357 WBI56_RS12985 WP_338730599.1 2516744..2517154(-) (nucA/comI) [Bacillus subtilis strain MC4-2]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTTCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAGCAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGCTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGTCGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAACAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

57.576

97.059

0.559